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Showing 1 to 15 of 15 for “"DNA binding specificity"”.
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DNA Binding Specificity of Mu Transcription Factor C and Crystallization of C : DNA Complex
… aims of this study was, (1) To understand the binding specificity of C and determine a possible consensus sequence for C binding, and (2) To crystallize the C : DNA complex as a first step towards structure determination. In previous work, single base substitution mutations in Plys identified …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… (REPSA), we identified the preferred DNA-binding motif for TTHB099, 5'-TGT(A/g)n(t/c)c(t/c)(a/g)g(a/g)n(T/c)ACA-3', and mapped potential binding sites, and regulated genes within the <em>T. thermophilus</em> HB8 genome. Comparisons with expression profile data in TTHB099-deficient and …
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DNA-binding specificity of the E2F gene family, and cloning and characterization of a novel family mamber
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 1999.
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Analysis of genes involved in cell-specific control of programmed cell death in Caenorhabditis elegans
… encode transcription factors. By sequence and DNA-binding specificity CES-1 is a member of the Snail family of zinc-finger proteins, and most similar to the Drosophila protein Scratch. CES-2 is a bZIP protein closest in sequence and DNA-binding specificity to members of the vertebrate PAR …
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Dissecting genetic determinants of transcription factor activity
… We exploit prior knowledge about the in vitro DNA-binding specificity of a TF in order to map the loci (`aQTLs') whose inheritance modulates its protein-level regulatory activity. The second study is to identify regulatory mechanisms underlying tumorigenesis in mice by using genotyping and gene …
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Old protein, new perspective : the role of Oct-1 during embryonic development and lymphopoiesis
… regulation. To date, only two octamer-binding proteins have been identified in B cells, the ubiquitously expressed Oct-1 and the lymphoid restricted Oct-2. While biochemical evidence suggests that Oct-i and Oct-2 exhibit identical DNA binding specificity, their respective roles in vivo …
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Analysis of Structure-Function Relationships in *Lambda and HK022 Site -Specific Recombination
… a three-dimensional model of the Lambda Int core-binding (CB) domain. The model suggests that all prokaryotic Tyrosine recombinases share a common CB domain fold, and facilitates interpretation of pre-existing genetic and biochemical data by providing a structural framework within which mutant …
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Evolution-Inspired Design of Bacterial Biosensors
… to interrogate structural features that confer DNA-binding specificity to the TetR family of TFs. Finally, in the third portion of this work, we develop new methods for analyzing signal transduction in bacterial TCSs. We characterize interactions between membrane-bound sensor histidine kinases …
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Discovery of a DNA-binding Consensus and Potential Genomic Regulatory Binding Sites for the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359
… gene transcription. Currently, knowledge of the DNA-binding specificities and genes regulated by many TFs, including those of well-studied model organisms such as <em>Escherichia coli</em> and <em>Thermus thermophilus,</em> remains incomplete or lacking which renders gaps in the understanding of …
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Molecular Dynamics Study of Hormone Receptors Binding DNA and Hormones
… the dynamic structural properties of modified DNA, binding of the estrogen receptor to specific and non-specific DNA, and binding/unbinding of hormones to/from retinoic acid receptor and thyroid hormone receptor. In the DNA dodecamer, d(CGCGAATTCGCG), two Adenine residues, individually or …
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Transcriptional Regulation of Chicken Apolipoprotein A-I Gene Expression
… of functional domains of v-Jun indicates that DNA binding specificity of vJun and its ability to heterodimerize with diverse partners are absolutely required for repression of apoA-I transcription. Further, the sequences between amino acid residues 108 to 128 in the amino terminus of v-Jun …
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Genetic strategies to manipulate meiotic recombination in Arabidopsis thaliana
… attempted to direct meiotic recombination using DNA double strand breaks targeted to specific sites. This project used a spo11-1 mutant, which is unable to produce the endogenous meiotic DNA DSBs that normally mature into crossovers. Instead, TALFokI nucleases (TALENs) were expressed from meiotic …
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Identification and Characterization of Transcriptional Regulatory Elements for Liver-Specific Expression of Rabbit CYP2C Genes
… bp upstream of their mRNA initiation sites. Both DNA binding and functional analysis strongly suggest that this hepatic specific element is a binding motif of a liver-enriched transcriptional factor hepatocyte nuclear factor 4 (HNF-4), since it was recognized by a nuclear protein with the same …
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Regulation of segment polarity genes in the head region of different arthropods
… site for the HLH-COE factor Collier. This binding site is essential for the function of the ic-CRE that also depends on collier activity. DNA-binding specificity of Collier to this endogenous sequence was further supported in vitro. Therefore, Collier directly controls expression of the …
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Specific Binding Affinity of the Non-Catalytic Domain of Eukaryotic Like Type IB Topoisomerase of vaccinia Virus
… that alter supercoiling in double stranded DNA (dsDNA) during transcription and replication and. <em>vaccinia</em> and the closely related poxvirus <em>variola</em> virus, at 314 amino acids in length, encode the smallest of the type I topoisomerases(TopIB). TopIB is a two domain protein …