Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"DNA barcodes"”.
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Tracking Floral Visitation Using DNA Barcodes
… IPT, one promising alternative is the use of DNA barcoding. Because floral visitors eat, collect or unknowingly obtain pollen, nectar and other floral tissues, plant DNA should be recoverable from their bodies, permitting molecular identification of pollen loads. To assess the utility of plant …
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Exploring Species Diversity and Molecular Evolution of Arachnida through DNA Barcodes
… of molecular evolution in Arachnida through DNA barcoding. The first chapter assesses mite species richness through comprehensive sampling at a subarctic location in Canada. Barcode analysis of 6279 specimens revealed nearly 900 presumptive species with high rates of turnover between major …
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Testing the utility of the consortium for the barcoding of life's two 'agreed upon' plant DNA barcodes, matK and rbcL
DNA barcoding is the use of short standardized regions of DNA to identify unknown specimens to species. Currently, the zoological community has agreed that cytochrome oxidase I subunit 1 (COI), a mitochondrial gene region, will serve as the barcode region for all animal taxa. Due to oftentimes …
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DNA barcoding of forensically important blow flies (Diptera: Calliphoridae) within the Western Cape of South Africa
… step towards estimating post mortem interval. DNA barcoding can aid in the identification of unknown forensically relevant species, and this requires the comparison of DNA barcodes to reference data from known species. However, there is a lack of DNA barcode reference data of forensically …
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Evolutionary Relationships Among Duiker Antelope (Bovidae: Cephalophinae)
… of their phylogeny. I test the ability of DNA barcodes to identify all taxa within this group. I then use mitochondrial and nuclear genes to estimate a multi-locus species tree and to date divergence times. DNA barcodes are unable to distinguish many sister taxa, calling into question the …
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Evaluating the Evolutionary and Genetic Relationships of the Andean Orchids of Northwestern Ecuador
<p>DNA barcoding is a molecular based technique used to separate and identify individual species. Here we establish a DNA Barcode library for the orchid flora of an Andean cloud forest in Northwestern Ecuador. The library contains 135 <em>matK</em> and 136 <em>rbcL</em> DNA Barcodes representing …
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Microbial profiling using metagenomic assembly
… a method has been established that links DNA sequences to the identification of organisms: DNA Barcoding. A similar approach has been developed for prokaryotes using target genic regions as markers for species identification and to profile communities. A key difference in these efforts is …
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Holomorphology and systematics of the eastern Nearctic stonefly genus Remenus Ricker (Plecoptera: Perlodidae)
… microscopy, color photomicrographs and COI DNA barcodes. Examination of all life stages has resulted in new comparative descriptions and a new key to adults. Remenus daniellae Verdone and Kondratieff, sp. n. is described from Great Smoky Mountains National Park, North Carolina and Tennessee, …
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Development of high-throughput nanoparticle screening technologies to facilitate the delivery of genetic therapies to non-liver cell types
… for LNP discovery must be applied. Utilizing DNA barcodes to allow for the testing of >100 LNPs simultaneously, we determined that the correlation between LNP biodistribution in vitro and in vivo was negligible. Motivated by these results, we developed three novel technologies to increase the …
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Αλληλούχηση του barcoding γονιδίου COX1 σε είδη ασπονδύλων, υδατικών συστημάτων της Ελλάδας και δημιουργία πρότυπης βάσης αναφοράς για μελέτες metabarcoding και περιβαλλοντικού DNA (eDNA).
… τελευταία στην Ευρώπη όσον αφορά την κάλυψη σε DNA barcodes για τα υδρόβια Κολεόπτερα, στις βάσεις γενετικών δεδομένων, περιορίζοντας έτσι την συμπερίληψη της ομάδας σε σύγχρονες μεθόδους βιοπαρακολούθησης, όπως το περιβαλλοντικό DNA (eDNA) και το metabarcoding. Στην παρούσα μελέτη, παρήχθησαν …
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Phylogenetic Analyses of Andean and Amazonian Tree Communities in Ecuador
… of this research was to evaluate the magnitude ofDNA barcode diversity among Amazonian and Andean tree species. Specifically, the objectives were to (1) evaluate community phylogenetic structure and correlate phylogenetic analyses with diversity metrics among Andean tree species along an …
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Innovations in Programmable Nucleic Acid Libraries and CRISPR Enrichment for Molecular Biology Applications
… complex libraries. By tagging genes with unique DNA barcodes and targeting these barcodes with in vitro transcribed sgRNAs, we successfully enriched three targeted barcodes by up to 1,094-fold. However, BAR-CAT scalability was limited beyond 12 targeted barcodes due to challenges with excessive …
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Investigating the natural history and predator complex of the native pine bark adelgid (Pineus strobi) in southwestern Virginia
… during a two-year survey. Morphology and DNA barcoding were used for identification. Species of predators found included: Laricobius rubidus (Coleoptera: Derodontidae), a native adelgid specialist, and two species from the dipteran family Chamaemyiidae, Leucopis piniperda Malloch and L. …
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Development of new tools and applications for high-throughput sequencing of microbiomes in environmental or clinical samples
… for preparing libraries from sheared genomic DNA with tunable size distributions using solid phase reversible immobilization (SPRI). This size selection captures DNA fragments of the necessary length to enable the generation of overlapping reads when sequenced from both ends. The lower-quality …
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Role of plastid markers in environmental studies on the example of the endangered species Cistus heterophyllus
… regions. Important is also the type of the DNA as source of our marker. Plastid DNA is preferred in plant phylogenetic projects whereas the analysis of hybridization events requires markers proceeding from nuclear DNA. However, in case of rare species, scientist encounter a lack of sequence …