Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"DNA assembly"”.
-
High throughput pin-tool based automated DNA assembly
… enabled by molecular biology techniques such as DNA assembly and molecular cloning. Researchers currently perform these techniques manually in wet-labs, which is expensive, time-consuming, requires extensive training and is unreliable. This thesis demonstrates a working robotic automation system …
-
New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… developed enabling the construction of plasmid DNA. This technique allows DNA assembly and manipulation (insertion, substitution and/or deletion) at any location of a vector. λ-PCR addresses the need for an easy, highly-efficient, rapid and inexpensive tool for genetic engineering and overcoming …
-
A rapid, flexible and scalable DNA assembly platform for genome engineering and regulated gene expression applications in Plasmodium falciparum
… requirement in this framework is the efficient assembly of donor vectors for modifying target loci and installing the necessary regulatory parts. This necessitates cloning several large, [A+T]-rich P.falciparum genomic regions that can be quite tedious and rate limiting. In this document, we …
-
Development of a new DNA-assembly method and its application for the establishment of a red light-sensing regulation system
In der hier vorgelegten Doktorarbeit wurde eine Strategie zur schnellen, einfachen und zuverlässigen Assemblierung von DNS-Fragmenten, genannt AssemblX, entwickelt. Diese kann genutzt werden, um komplexe DNS-Konstrukte, wie beispielsweise komplette Biosynthesewege, aufzubauen. Dies dient der …
-
Rational Engineering of Expression Level of Multi-Gene Systems encoding Natural Product Biosynthesis in Streptomyces
… BGCs by refactoring and physically piecing DNA fragments together in a hierarchical manner. The two core technologies are (i) a high-throughput DNA assembly pipeline for high GC organisms and (ii) synthetic genetic elements to control gene expression in Streptomyces. As a proof of concept, a …
-
Growing a second skin : towards synthetic biology in product design
… goals are pursued in two distinct projects: DNA assembly in 3D printed fluidics and Mushtari, a fluidic wearable designed to contain cyanobacteria and E. coli cultures. Contributions include (1) characterizing the resolution of three 3D printing methods for creating fluidic channels, (2) …
-
High throughput single molecule in situ-verified nucleic acid synthesis
… in computer programs, and specified at the DNA level. Subsequently, DNA encoding the design must be built to specification and tested for desired functionality in vivo or in vitro. In current practice, building DNA, by de novo DNA synthesis and related methods, is a rate limiting and costly …
-
Studying DNA-Directed Nanoassembly, Hybridization and Mismatch-Discrimination via Plasmon Scattering of Gold Nanoparticles
… (nAu) and the specific recognition attributes of DNA to investigate the nAu-DNA assembly formation, and to apply the nanoassemblies for DNA detection. The nanoassembly structures, successfully correlated to the LSPR wavelength shift and the plasmon color change, showed that their homogeneous …
-
Automation of the golden gate assembly reaction at low volumes using pin tools
The ability to quickly and reliably create new DNA constructs has been pushing forward many fields, including synthetic biology. This thesis investigated increasing the capability of creating genetic circuits through cost reduction and automation using Golden Gate assembly as a model assembly …
-
DNA Directed Organic Semiconductor Interactions Controlling Excitons, Charge Transfer States, and Singlet Fission
… between molecules. Yet, control over spatial assembly of organic semiconductors is challenging. Large aromatic molecules will self-assemble into extended structures with no size or positional control. Deterministic interactions between two different semiconductor molecules is even rarer. In …
-
Development and applications of programmable DNA-guided Argonaute-based artificial restriction enzymes
… class of nuclease enzymes which recognize short DNA sequences and cleave DNA molecules at or near their recognition site. Type II restriction enzymes are capable of cleavage of DNA at a fixed location with respect to their recognition sequence and some type II restriction enzymes are able to …
-
De novo sequencing, assembly and analysis of the genome and transcriptome of the nematode Panagrolaimus superbus
… RNA extraction. Nor- malised and unnormalised cDNA libraries were prepared from this mixed population. A de novo assembly of the transcriptome was generated using a variety of assembly programs and strategies. A Sanger sequenced expressed sequence dataset comprising 3,982 unigenes was fully …
-
Frameworks for reprogramming early diverging land plants
… the compilation of a gene-centric registry of DNA parts for engineering (MarpoDB). I describe the development of Loop assembly, an efficient and standardised DNA assembly system based on Type IIS restriction enzymes for recursive fabrication of DNA circuits with high efficiency. MarpoDB was …
-
Synthetic Auxin Engineering: Building a Biofoundry Platform
… Next, I describe and validate a platform called AssemblyTron, which deploys liquid handling robotics for DNA assembly and can serve as the foundation of a biofoundry platform. I then introduce TidyTron, which is a protocol library for automated wash and reuse of single use lab plastics to promote …
-
Structural and functional effects of histone variant, H2A.Bbd, on the nucleosome core particle
In eukaryotic cells, DNA is packaged into a protein-DNA assembly called chromatin. The basic subunit of chromatin is the nucleosome core, which is composed of 147 base pair (bp) of DNA wrapped in 1.65 turns around a histone octamer containing two copies each of the four core histone proteins (H2A, …
-
Bio-Nano Interfaces: Enzyme Immobilization for Biomimetic Energy Harvesting
… area that allowed for the attachment of protein/DNA assembly. The specificity of the enzyme immobilization was controlled by small protein structural motifs, called zinc fingers (ZnF) that bind to specific dsDNA sequences and may be genetically bound to small laccase (SLAC-3ZnF) or other redox …
-
Harnessing Saccharomyces cerevisiae Genetics for Cell Engineering
… of Reiterative Recombination, a robust in vivo DNA assembly method relying on homing endonuclease-stimulated homologous recombination. Reiterative Recombination elongates constructs of interest in a stepwise manner by employing pairs of alternating, orthogonal endonucleases and selectable …
-
Multiple sequence recovery: theory and applications
… plays an imperative role in areas such as DNA coding for data storage and computational biology. Since many algorithms for DNA assembly and error correction use k-mers, or substrings of length k, of input sequences, we first consider the task of reconstructing multiple source strings from …
-
Medical Engineering Across Length Scales Using Structural and Analytical Biomaterial Nanotechnology
… scopes are connected by the application of DNA and lipid nanotechnology for structural and analytical purposes. The first objective of this thesis describes the development of a DNA-liposome composite structure inspired by the highly ordered architecture of clathrin self-assemblies. A DNA …
-
Electrochemical and genetic tools for analysing and engineering bioenergetic processes in cyanobacteria
… making iron availability essential for their assembly, function, and maintenance. This research revealed that outer membrane vesicles selectively uptake Fe<sup>3+</sup> ions, answering a longstanding question on cyanobacterial physiology. Finally, in addition to these electrochemical …
Page 1 of 2