Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"DNA assembler"”.
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Enabling Natural Product Biosynthesis With Novel Synthetic Biology Tools
… research and development. The first tool is DNA assembler, which allows rapid construction of large recombinant DNAs in a single-step fashion through yeast in vivo homologous recombination. Further applications of DNA assembler to construct a highly versatile shuttle system and assemble an …
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Rekonstrukce repetitivních elementů DNA
… práce obsahuje hlavní klasifikaci repetitivní DNA a představuje implementaci nového de novo assembleru, zaměřeného na hledání a sestavení LTR retrotranspozonů a satelitní DNA. Assembler přijímá na svém vstupu krátké ready (single nebo pair-end), získané sekvenátory druhé generace (NGS). Tento …
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Discovering novel natural products from bioactive plants and bacteria
… 94 clones and 27 clones were sequenced from the cDNA libraries created from the leaves of E. camaldulensis and E. robusta, respectively. Five unique putative Type III PKSs genes were identified. Two full-length genes were cloned and the biochemical characterization of these genes indicated their …
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Metabolic engineering of Saccharomyces cerevisiae for efficient ethanol production from pentose sugars
… utilization pathways using our newly developed DNA assembler method. Using the library we generated, clones with better combinations of enzyme homologues were identified using a colony size based screening method. To fine-tune the expression level of the three genes in a heterologous xylose …
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Characterization and reconstitution of enzymes and pathways in the biosynthetic production of nitroaromatic compounds
… of constructs using several methods such as DNA assembler, Golden Gate and Gibson assembly. Expression of the gene cluster’s proteins was observed in E.coli, while feeding studies confirmed the production of nitroaromatic compounds from the cluster. A product detection method based on …