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Showing 1 to 16 of 16 for “"DNA aptamer"”.
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Immobilization Of The Anti-Thrombin DNA Aptamer: Attachment, Optimal Packing Density And Kinetics
Aptamers have been touted as viable complements to antibodies as molecular recognition elements in diagnostic assays. Several studies have demonstrated aptamer applicability in both homogeneous and heterogeneous assay formats. However, very few studies have been performed to investigate the …
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Catalysis by DNA enzymes: roles of lanthanide(III) ions and DNA aptamer modules
For many years scientists considered DNA and RNA to be merely the means for the storage and transmission of genetic information. Catalytic roles of some RNA molecules, called ribozymes, were discovered in the early 1980s. Considering structural similarities between RNA and DNA, it was reasonable to …
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Two-dimensional infrared spectroscopy of nucleic acids : application to tautomerism and DNA aptamer unfolding dynamics
… the structural dynamics of thrombin-binding aptamer (TBA), which is a 15mer DNA folded into a guanine-quadruplex (G-quadruplex). The 2D IR spectral signatures of G-quadruplex were established, and T-jump transient 2D IR was employed to investigate the unfolding dynamics of TBA. A mechanism of …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… specificity. </p> <p> In this thesis work, DNA aptamer candidates with binding specificity for the wild-type bacterial H69 were selected. The 84-nt-long DNA aptamer (H69DNAapt18) that was identified from a DNA library with a 40-nt randomized region has the sequence …
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Aptamers that bind SARS-CoV-2 spike protein for COVID-19 rapid diagnostics and therapeutics
… as well as disease surveillance. To that end, DNA aptamer-based rapid diagnostics show promise as cost-effective, shelf-stable alternatives to those based on antibodies. In the introduction, I discuss what DNA aptamers are, how they are discovered, and how they are applied to biosensing. In …
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High-Resolution Electrohydrodynamic Jet Printing Methods for Applications in Electronics and Biotechnology
… various material inks, including biomaterials (DNA, protein), suspensions of single walled carbon nanotubes/nanoparticles, and solutions of conducting/insulating polymers, demonstrates some of the features of the methods. Simple devices, such as transistors that use aligned arrays of single …
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An Optical Aptamer-Based Carbon Nanosensor for Cortisol Detection
… This work presents the development of an optical aptamer-based carbon nanosensor for the detection of cortisol. The sensor utilizes single-walled carbon nanotubes (SWCNTs) functionalized with a specific DNA aptamer to achieve high specificity and sensitivity. This work touches on the importance of …
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Selecting DNA aptamers for 17β-estradiol
… a promising approach which employs the use of DNA aptamers for the detection of E2. DNA aptamers are single stranded DNA molecules which are capable of binding target molecules with high affinity and selectivity. The aim of this project is to develop DNA aptamers that would function as sensors …
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Explorations of DNA-Single-Walled Carbon Nanotube Interactions to Develop Multiplexed Molecularly Specific Biosensors for Inflammation
… through innovative use of single-stranded DNA (ssDNA) interfaces.</p> <p>First, an IL-6-specific DNA aptamer was employed as both a dispersing agent and recognition probe for SWCNT fluorescence sensing. Sequence modifications, including anchor domains, truncations, and thermally induced …
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Silica Nanoparticle-based Novel Fluorescent Biosensors with Conjugated Polyelectrolyte-sensitized Signals
… Label-free ATP detection was designed using DNA aptamer/ATP specific interaction as the recognition event and intercalator ethidium bromide (EB) as signal reporter. The detection limit and assay selectivity tetrahydrofluorene-sensitized EB emission via a fluorescence resonance energy transfer …
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High Throughput Screening of Aptamers
<p>Aptamers are functional oligonucleotides that are discovered through directed evolution. With affinities for their targets similar to antibodies, aptamers are useful in biosensors, diagnostics and therapeutics. Three elements of natural selection--variation, selection, and replication afford …
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Nucleobase-modified deoxyribozymes for amide and peptide hydrolysis
… into complex structures to enable catalysis. DNA is primarily double-stranded and is non-catalytic in nature. However, considering the structural similarity to RNA, single-stranded DNA should also be able to form complex structures and perform catalysis. In fact, artificial DNA enzymes …
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Aptamer-Based Detection of Salivary Melatonin for Circadian Rhythm Sleep Disorders Monitoring
… in patients with circadian rhythm disorders. Aptamers, due to their numerous advantages such as small size and structural flexibility in tuning affinities, are preferable biorecognition molecules and can provide detection of melatonin with high sensitivity and specificity. The thesis aims to …
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Selection and characterization of DNA aptamers for estradiol and ethynylestradiol for aptasensor development
… A promising alternative makes use of DNA aptamers as molecular recognition elements. In order to evaluate the potential of DNA aptamers and aptasensors to detect small organics in natural waters, the following objectives were pursued: (1) critically review DNA aptamers and aptasensors …
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DNA as a functional molecule for control of nanomaterial morphology and for biosensing
DNA, traditionally known as the carrier of genetic information has proven that it can be utilized as a key player for self-assembly, material synthesis and molecular sensors. Given the ease of synthesis and modification, precise control over sequence and specificity in base pairing, the number of …
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Applications of functional nucleic acids in imaging, sensing and drug delivery and investigations of DNAzyme-metal interactions
… including nucleic acids catalysts (ribozymes and DNAzymes) and ligands (aptamers), have been discovered in nature or isolated in a laboratory through a process called in vitro selection. They are nucleic acids with functions similar to protein enzymes or antibodies. They have been developed into …