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Showing 1 to 7 of 7 for “"DNA affinity chromatography"”.
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Advances in DNA Affinity Chromatography
<p>Different aspects of DNA affinity chromatography such as DNA complexity heparin elution, the Bi-column method and the oligonucluotide trapping method were studied. The complexity (length) of a DNA sequence attached to an affinity chromatography column affects column retention, and the purity of …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
… utilizing heparin column, size-exclusion and DNA-affinity chromatography, 2-D gel electrophoresis and mass fingerprinting to identify proteins that bound the attachment sites of CTnDOT (attDOT ) and NBU1 (attNI), and stimulated integration in vitro for both DNA elements. The proteins isolated …
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Purification and Characterization of OTF-1, A Transcription Factor Regulating Cell Cycle Expression of a Human Histone H2b Gene: Demonstration of its Functional Identity with NF-III, a Factor Required for Efficient Initiation of Adenovirus DNA Replication
… purification was achieved through the use of DNA affinity chromatography, and the factor was unambiguously identified by renaturation of activity following SDS-polyacrylamide gel electrophoresis. The purified factor retained the ability to efficiently stimulate H2b transcription in a …
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Identification of Proteins Involved in Chloroplast DNA Replication
… <p>Chloroplast nucleoids (ct-nucleoids) are DNA/protein complexes involved in compacting the chloroplast genome, and may play a role in regulating DNA replication. Ct-nucleoids were isolated from young soybean plants and separated by 2-D gel electrophoresis. Gel spots were excised and …
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Purification and characterization of a poly(dG).poly(dC)-binding protein from Parenchinus angulosis
… suGF1 was purified to near homogeneity by DNA-affinity chromatography. The purification procedure involved a cation exchange step, followed by poly(dG).poly(dC)-affinity chromatography, and finally by affinity chromatography utilizing multimerized specific DNA-binding sites of suGF1. The …
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Transcriptional Regulation of the Glycogen Phosphorylase-2 Gene in <I>Dictyostelium discoideum</i>
… Purification of TF2 was achieved by ion-exchange chromatography, DNA affinity chromatography, gel filtration chromatography, and preparative SDS-PAGE. SDS-PAGE analysis indicated an apparent subunit molecular weight of 28 kDa. The apparent molecular weight of the native protein as estimated by gel …
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Characterisation of CIS- and trans-acting factors that regulate the human alpha 2(1) procollagen gene
… has been correlated with differential in vitro DNA-protein interactions on the basal promoter region between -100 and -67. A 23 bp region of the a.2(1) procollagen promoter encompassing the G/CBE (CCTCCATTGG) and the Ctv'IE (GGAGGCCCTTTT) has previously been shown to engage in specific DNA …