Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"DNA Viruses"”.
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Metagenomic approaches for examining the diversity of large DNA viruses in the biosphere
The discovery of large DNA viruses has challenged the traditional perception of viral complexity due to their enormous genome size and physical dimensions. Previously, viruses were considered small, filterable agents until the discovery of large DNA viruses. Among large DNA viruses, the phylum …
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The evolutionary impacts of secondary structures within genomes of eukaryote-infecting single-stranded DNA viruses
… immune responses. Although computational DNA/RNA folding methods based-on free energy minimisation approaches are capable of predicting structures that form within virus genomes, these methods are not entirely accurate. Notably, many of structures that are accurately predicted will likely …
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Investigation of local South African avipoxviruses as potential vaccine vectors
Avipoxviruses are large, genetically diverse DNA viruses which are particularly desirable for use as vaccine vectors as a result of their excellent safety profile and host range restriction. In this study, 8 novel South African (SA) avipoxvirus isolates were characterized. They could be divided …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… through genomic integration of transgenic DNA via genome editing (e.g., CRISPR\Cas9) or integrating viral vectors (e.g., retrovirus, lentivirus). Integration-based approaches risk insertional mutagenesis and oncogene activation, yielding unpredictable, irreversible consequences. Inspired by …
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Mechanistic studies of the Class I ribonucleotide reductase from Escherichia coli
… providing the monomeric precursors required for DNA replication and repair. The class I RNRs are found in many bacteria, DNA viruses, and all eukaryotes including humans, and are composed of two homodimeric subunits: R1 and R2. RNR from Escherichia coli (E. coli ) serves as the prototype of this …
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A Role for Mindbomb 1 in Adenovirus Genome Delivery
… pore is a critical step in the lifecycle of DNA viruses, many of which must successfully deposit their genomes into the nucleus for replication. Viral capsids strategically navigate this vast distance (and all subsequent lifecycle steps) through the coordinated hijacking of a number of …
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Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
… (HBV) is the prototype member of the family Hepadnaviridae that consists of enveloped, partially double stranded DNA viruses that specifically target hepatocytes for viral replication. Although a vaccine has been available for more than 20 years chronic HBV infection afflicts 350-400 million …
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Characterization of the antiviral activity of cyclosporine A against dengue virus
… antiviral effective against various RNA and DNA viruses, including DENV. However, its antiviral mechanism is not fully understood, and its effectiveness against DENV-2 in different cell lines has yet to be explored. In this study, we evaluated the antiviral activity and mechanism of CsA …
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Innate Immune Sensing and Signaling of Cytosolic DNA
In eukaryotic cells, DNA is normally confined within the nucleus and mitochondria. DNA exposed in the cytosol is a danger signal that warns the host of invading microbial pathogens and triggers innate immune responses including the production of type-I interferons (IFNs). Endogenous DNA that is …
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Pseudovirions in the Study of Papilloma- and Polyomaviruses
The papilloma- and polyomaviruses are small DNA viruses that infect humans. Some members of these virus families can cause cancer in experimental animals and some are also established as carcinogenic to humans. Detection of specific antibodies to these viruses allows tracking past and present …
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Conserved Residues in Murine Papillomavirus E2 Regulate the Viral Life Cycle
Papillomaviruses (PVs) are small, non-enveloped DNA viruses that infect the stratified epithelia. Once an infection is initiated, the virus must successfully navigate the three stages of its life cycle: establishment, maintenance, and vegetative amplification. A major mechanism of regulating this …
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Electrophoretic Mobilities of Topoisomers of ΦX174 DNA
… demonstrated the existence of several circular DNA forms that appear in cells infected with the bacteriophage ΦX174.</p> <p>Wt/<em>am</em>3 heteroduplex molecules containing a single base mismatch (relaxed RFI molecules) were constructed <em>in vitro</em>, purified by sedimentation, and analyzed …
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Molecular characterization of viruses infecting potato and vegetables in Iraq
… published molecular information concerning plant viruses from Iraq, this study was initiated to investigate the diversity of viruses infecting potato and vegetables in Iraq on a molecular basis. Based on the economic importance and incidence worldwide, eight virus genera were investigated in 175 …
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Characterization of the anti-viral effects inhibited by the vaccinia virus K1 protein
… member of the poxvirus family of double-stranded DNA viruses, is well-known as a highly effective vaccine against variola virus, the causative agent of smallpox. Poxviruses encode many strategies to evade the host immune and anti-viral response. One such host anti-viral response, activation of the …
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Sensing Foreign DNA: The Role of DNA-PKcs in Human Anti-viral Innate Immunity
… patterns (PAMPs), such as viral genomic DNA, which is a major PAMP during DNA virus infection. Viral DNA sensing leads to the activation of the STING-TBK1-IRF3 signalling axis and the production of type I interferon. Previously, our work identified the non-homologous end-joining protein …
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Nucleosome-independent permanently condensed chromosome: investigation of novel nuclear organisation in the dinoflagellates
… only dinoflagellates and numerous marine large DNA viruses. I will address two issues in this thesis, 1) what are the properties of the protein DVNP, and can we infer if and how DVNP manages the permanently condensed chromosome state; and 2) how do other proteins accommodate this drastically …
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Evolutionary History of Immunomodulatory Genes of Giant Viruses
Nucleocytoplasmic large DNA viruses (NCLDVs) have genome sizes that range from around 100 kilobases (kb) to up to 2.5 megabases, and virion sizes that can reach up to 1.5 μm. Their large size in both of these contexts is atypical and defies the traditional view that viruses are streamlined, …
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Natural Killer Cell Recruitment and Activation Following Orthopoxviral Infection
<p>The orthopoxviridae are large, complex DNA viruses with important historical and clinical significance. Members of this genus encode numerous proteins known to subvert the immune response to infection, providing a useful system to probe required host responses for resistance and survival. …
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Multiple Functions of BRD4 in E2 Mediated HPV Transciptional Regulation
Human papillomaviruses (HPVs) are DNA viruses that cause benign and malignant tumors of epithelial origins. Expression of HPV-encoded E6 and E7 oncoproteins is controlled by the viral E2 protein, which plays a dual role in gene activation and repression. Recently, we identified …
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Structural and Computational Studies of RNA Polymerase
… within -35 element induce an AA/TT-tract like DNA geometry, allowing for indirect promoter recognition despite the absence of direct protein/DNA interactions with several highly conserved DNA bases. (2) Created comprehensive multiple sequence alignments for the two bacterial large subunits …
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