Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 43 for “"DNA Structure"”.
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Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability
… In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a Z-DNA or H-DNA, this can lead to genetic instability in the form of …
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Effect of Naturally Occurring DNA Modifications on DNA Structure and Packaging
In eukaryotes, the genomic double-stranded DNA (dsDNA) coils around histones to form nucleosomes. Arrays of these nucleosomes bundle together to generate chromatin. Most DNA-related processes require interactions between chromatin-protected DNA and cellular machinery. Access of cell machinery to …
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The potential of DNA structure to provide a resource for the creation of art
… and scientists, focusing on the visualisation of DNA. The project will identify their shared approaches to its representation, and will explore the history of DNA as an iconic form. An additional purpose of this study is to analyse the importance of the role of collaboration between scientists and …
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Interactions Between Platinum-Intercalator Hybrid-Agents and The Human Telomeric G-Quadruplex
Telomeres are stretches of non-coding DNA sequences in complex with multifunctional proteins, which form the termini of the chromosomes in most eukaryotic organisms. Two important roles of the telomeres are to prevent chromosome end-to-end fusion and loss of genetic information. While the gradual …
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Interaction of B-DNA and Monovalent Cations: Theory and Practice in X-Ray Crystallography
… the solvent/counter-ion region of x-ray crystal structures are raised. The ambiguity in the identity and occupancy of the molecular and atomic species in this region is explored experimentally. Anomalous scattering is proposed as a possible method for resolving this ambiguity. To this effect, the …
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Structure of the amplified 5-enolpyruvylshikimate-3-phosphate synthase gene in glyphosate-resistant carrot cells and attempts to isolate the anthranilate synthase gene from plants
Analysis of the structure of amplified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) DNA of carrot suspension cultured cell lines selected for glyphosate resistance was carried out to determine the mechanism of gene amplification in this plant system. Cloning of the EPSPS gene and …
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REPAIR AND EFFECTS OF THE 8-OXOG LESION IN DNA
Eukaryotic DNA is packaged in a condensed state with histone proteins. The minimal structural unit within packaged eukaryotic DNA is the nucleosome core particle (NCP). The NCP consists of a 146 bp DNA fragment wrapped around an octamer of histone core proteins. Nucleosome core particle formation …
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REPAIR AND EFFECTS OF THE 8-OXOG LESION IN DNA
Eukaryotic DNA is packaged in a condensed state with histone proteins. The minimal structural unit within packaged eukaryotic DNA is the nucleosome core particle (NCP). The NCP consists of a 146 bp DNA fragment wrapped around an octamer of histone core proteins. Nucleosome core particle formation …
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Sequence Effects and Ligand Selectivity in Targeting Non-Canonical DNA Structures
G-Quadruplex (G4) DNA is a secondary DNA structure that can occur in G-rich DNA sequences. These structures, which are compact and diverse, have been implicated in a wide range of processes including telomere maintenance, transcriptional control, and DNA replication and repair. In order to target …
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Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor
… since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the displacement of a nucleosome positioned over this area. A poly( dG).poly( de)-binding protein (suGF1) has previously been identified and purified on a small scale from …
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Characterization of inter-genomic and telomeric G-quadruplex structures through atomic force microscopy
The G-quadruplex DNA structure, a secondary folding motif of DNA, is found throughout the genome. The dynamics of these structures has been seen to affect processes such as telomerase elongation and regulation of gene transcription. Typical investigation techniques require these structures to be …
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The development of a nanoscale Coulter counter for rapid genetic sequence recognition
… genetic sequences of deoxyribonucleic acid (DNA); the general approach used to accomplish sequence recognition is a refinement of the resistive pulse technique. Commercial Coulter counters fabricated with sub-micrometer apertures can size particles with roughly twenty nanometers of …
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Stability of Nucleic Acid Secondary Structures and Their Contribution to Gene Expression
… encoded in the bacterio-opsin gene {bop) (1). DNA sequence analysis and predictive folding algorithms suggest that the first twenty-five bases of the bop gene mRNA can form a secondary structural element (a "stem-loop"). Using biophysical methods, the goal was to determine if the stem-loop …
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Transient Optical Picocavities Within Coupled Plasmonic Nanostructures
… from a molecule placed into NPoM using a DNA structure. The DNA complicates the response, which is time variant with transient features suggestive of possible picocavity formation. Picocavities are transient atomic scale features on the metal surfaces which further confine fields …
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Micropatterned cell arrays for detecting DNA damage
Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are important for risk assessment and identifying therapeutic interventions. One …
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Nanoparticle probes for the study of biomolecular interactions
… for the detection of single and double stranded DNA sequences. The oligonucleotides are modified to incorporate locked nucleic acid (LNA) ribose sugars, affording highly advantageous affinity and selectivity properties. Hybridisation of the probes with the target sequence was monitored …
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Molecular Dynamics Study of Hormone Receptors Binding DNA and Hormones
… the dynamic structural properties of modified DNA, binding of the estrogen receptor to specific and non-specific DNA, and binding/unbinding of hormones to/from retinoic acid receptor and thyroid hormone receptor. In the DNA dodecamer, d(CGCGAATTCGCG), two Adenine residues, individually or …
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The Evolution of Glioblastoma: A Single Case Study
… promoter methylated cell lines. Moreover, the DNA analysis of the patient showed one shared mutation in high copy number in all of the nine samples: a mutation in EGFR G598V. We hypothesise that subsequent tumours developed from an original clone bearing this mutation. This mutated oncogene is …
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