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Showing 1 to 20 of 435 for “"DNA Replication"”.
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Chromatin Dynamics During DNA Replication
… maintained with respect to promoters.<br/><br/>DNA and chromatin replication are coupled processes whereby chromatin is disrupted ahead of the replication fork and nucleosomes are rapidly assembled on the nascent DNA template. Classically it has been observed that nascent chromatin is more …
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ONCOGENE-INDUCED ALTERED DNA REPLICATION DYNAMICS
… cycle permanently. OIS results from a robust DNA damage response (DDR) activation due to oncogene-induced hyper-proliferation. By performing a whole genome analysis of DNA replication dynamics occurring upon oncogene activation, I discovered that oncogene activation alters DNA replication by …
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DNA-Protein Interactions in f1 DNA Replication
<p>This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately …
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Mechanism of Mcm10 function during DNA replication
… do so with limited errors. In eukaryotic cells, DNA replication is accomplished by a multi-stage process involving numerous protein assemblies. The core component of this process is the replicative DNA helicase, Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 …
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Identification of Proteins Involved in Chloroplast DNA Replication
… <p>Chloroplast nucleoids (ct-nucleoids) are DNA/protein complexes involved in compacting the chloroplast genome, and may play a role in regulating DNA replication. Ct-nucleoids were isolated from young soybean plants and separated by 2-D gel electrophoresis. Gel spots were excised and …
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Mitochondrial DNA replication in pre-implantation embryonic development
All eukaryotic cells possess mitochondrial DNA (mtDNA), which is maternally inherited through the oocyte, its replication being regulated by nuclear-encoded replication factors. It was hypothesised that mtDNA replication is highly regulated in oocytes, pre-implantation embryos and embryonic stem …
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Structural Studies of DNA Replication Machinery in Archaea
DNA replication is a fundamental process present in all living organisms. It is the means by which organisms transfer their genetic information to their offspring to ensure the propagation of their lineage. One of the essential features of DNA replication is to separate the DNA double helix to …
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Regulation of DNA replication by PP2A family phosphatases
In eukaryotes, DNA is replicated once and only once per cell cycle from hundreds of replication initiation sites (origins) across the genome. This process is strictly controlled as mis-regulation can cause replication stress and genome instability, characteristics of cancer. S phase …
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Developmental regulation of DNA replication in Drosophila melanogaster
… chromosomes fully or a failure to limit replication to once-per-cell-cycle may lead to aneuploidy and genomic instability. Variants of the archetypal mitotic cell cycle, utilizing conserved cell cycle machinery, are employed during metazoan development to achieve different aims. …
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The regulation of DNA replication during Drosophila development
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Developmental regulation of DNA replication initiation in Drosophila
… a powerful system for the study of metazoan DNA replication. Amplification produces 100kb gradients of amplified DNA through repeated rounds of origin firing and bidirectional movement of replication forks from these origins. The Drosophila Follicle Cell Amplicon at the cytological location …
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Electron microscopy of bovine Parvovirus DNA replication intermediates
Several low molecular weight DNA species have been isolated from autonomous parvovirus-infected cells that could·not be demonstrated in mock-infected cells. The DNA species were termed replicative forms and intermediates. The purpose of this study was to isolate and characterize the replicative …
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Biochemical mechanisms for selectivity governing RNA and DNA replication
Enzymes that catalyze DNA and RNA replication are biologically important and provide examples to understand enzyme specificity as the correct substrate and alternate substrates are well known. The DNA polymerase from bacteriophage T7 has been a model system for understanding high-fidelity DNA …
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Functions of RTF2 in DNA Replication and Checkpoint Control
… composition of the protein machinery that copies DNA, called the replisome, is dynamically regulated across S phase to promote the accurate and complete duplication of the genome before cell division. Work in our laboratory has uncovered one such dynamic process at replication forks experiencing …
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The Role of the Protein MTBP in DNA Replication
The initiation of eukaryotic DNA replication occurs at replication origins, licensed by loaded double hexamers of Mcm2-7 proteins. The action of two kinases, DDK and S-CDK, triggers the loading onto Mcm2-7 of Cdc45 and the GINS complex to form the replicative CMG helicase.<br/><br/>In yeast, the …
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Genome-Wide Dynamics of Chromatin Maturation Following DNA Replication
<p>All DNA-templated events, including replication and gene transcription, occur in the context of the local chromatin environment. The passage of the replication machinery results in disassembly of chromatin, which must be re-assembled behind the replication fork to re-establish the epigenetic …
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Uncovering Phosphorylation Circuitries that Control DNA Replication Fork Dynamics
… duplicate, and divide. Faithful duplication of DNA is required to pass the genetic information to the daughter cell. DNA replication is a fundamental process that frequently encounters challenges from exogenous and endogenous sources. Barriers to replication slow down DNA synthesis and threaten …
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Single molecule investigations into DNA replication, recombination and repair
Double-stranded DNA breaks (DSBs) is a principal cytotoxic damage that can be repaired by several well-known repair pathways. One of the most prominent routes is the homologous recombination (HR). The mechanism of repair has been studied primarily by biochemical analysis over the years. Recent …
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