Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"DNA Probes"”.
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Novel DNA probes for sensitive DNA detection
The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap …
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Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans
A DNA probe was developed for the identification of the periodontal pathogen, Actinobacillus actinomycetemcomitans. Chromosomal DNA was extracted from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a …
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THE ATTACHMENT AND CHARACTERIZATION OF DNA PROBES ON GaAs-BASED SEMICONDUCTOR SURFACES
… studied as substrates for deoxyribonucleic acid (DNA) attachment, the development of a new type of substrate, which is reproducible, stable, highly controlled and easily transferred to practical applications, is still needed. Recent studies have shown that As terminated GaAs-based semiconductors …
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Novel fluorescent nucleotides for metabolic labelling and for the construction of DNA probes
… nových nukleosidů, nukleotidů a příslušných DNA sond nesoucích různé fluorescenční značky pro bioanalytické aplikace. V první části práce byl pomocí Sonogashiry cross-kapling reakce syntetizován 2'-deoxycytidin a jeho trifosfát nesoucí imidazolinový fluorofor na bázi tryptofanu. Daný …
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Cytogenetic studies of testicular germ cell tumors with chromosome 12 specific DNA probes
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Detection of Babesia species in domestic and wild Southern African felids by means of DNA probes
… and differentiate between blood parasites using DNA probes. The RLB consists of three basic steps, the first being amplification of the variable region (V4) in the 18S rRNA gene using genus-specific primers where one is labelled with biotin. This is followed by a blotting step, where the …
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Construction and Use of Species-Specific Dna Probes for the Detection, Identification, and Enumeration of Human Colonic Bacteroides Species (Anaerobes, Hybridization, Microecology, Feces)
… which exploits differences in chromosomal DNA sequences among Bacteroides species. Random fragments of chromosomal DNA that are unique to each of 8 major colonic Bacteroides species were obtained and used to construct species-specific DNA probes. A procedure was devised whereby Bacteroides …
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Direct Quantitative Analysis of Multiple Micrornas
… approaches: 1) drag tags on the DNA probes to separate multiple hybrids and 2) single-strand DNA binding protein (SSB) in the run buffer to separate excess, unbound DNA probes from the hybrids. In the proof-of-principle work, I detected three miRNAs directly from a cell lysate …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… development of the molecular beacon, as a new DNA probe for DNA genotyping, DNA computation and biophysical studies of DNA conformations. Molecular beacons are single-stranded DNA molecules that form a stem-and-loop structure. A fluorophore and a quencher are grafted at their two ends to report …
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DNA-DNA Checkerboard Hybridization to Identify Bacteria in Human Plaque Samples
<p>Previous research has shown that using DNA probes to identify the presence of specific bacteria in human plaque samples is superior to the commonly used anaerobic cultural procedures. Some periodontal pathogens are either uncultivable or very difficult to grow, such as<em> Prevotella intermedia, …
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The development and evaluation of a polymerase chain reaction assay for the diagnosis of mycobacterium tuberculosis infections
… of sensitivity, specificity and rapidity. DNA probes, although rapid, only have a sensitivity similar to that of Ziehl-Neelsen stain. The polymerase chain reaction (PCR) has been shown to exhibit extreme sensitivity and specificity for a wide variety of infectious agents including virus, …
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Development of Novel Collection and Biosensor Detection System for Microbial Organisms
… to lyse the organisms and shear the genomic DNA in preparation for detection by a DNA biosensor. Initially, the specificity of the DNA probes was determined using polymerase chain reaction and gel electrophoresis. Probes shown to be selective for a particular bacteria or fungi were bound to …
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Single molecule pull-down of viral RNA-protein complexes
… DI RNA, we designed several short complementary DNA probes to capture single DI RNA molecules for detection with single molecule fluorescence microscopy. We demonstrate specific capture of viral DI RNA molecules using SiMPull, and could quantitatively measure the presence of interacting viral …
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Sensitive Multiplexed MicroRNA Spatial Profiling and Data Classification Framework Applied to Murine Breast Tumors
… The hydrogel posts are functionalized with DNA-probes that enable the detection of miRNAs across a large dynamic range (four orders of magnitude) and a limit of detection of 0.17 zeptomoles (1.7×10⁻⁴ attomoles). We applied our methodology coupled with a data analysis pipeline to K14-Cre …
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Development of an Optical Fiber Biosensor with Nanoscale Self-Assembled Affinity Layer
… reactions, monoclonal antibodies (IgG) or DNA probes are immobilized onto the surface of the ISAM-coated fiber, which form the core component of the biosensor. By immersing such biosensor fiber into a sample suspension, the immobilized antibody molecules will bind the specific antigen and …
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The detection of plasmid transfer genes in Rhizobium species : a thesis presented in partial fulfilment of the requirement for the degree Master of Science in Microbiology at Massey University, New Zealand
… for Sym plasmid transfer might be found, DNA probes were constructed from Agrobacterium tra genes. Three probes were constructed from DNA containing;- 1) traR, a gene which regulates other tra genes on the Agrobacterium tumefaciens plasmid pTiC58, 2) an OriT site, at which nickases cleave …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… the aetiology remains largely unanswered. Anti-dsDNA antibodies have been found intrathecally and bordering lesions in multiple sclerosis patients and in view of their known pathogenity in lupus nephritis the aim of this project was to further investigate their role in multiple sclerosis. Using …
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Pathological role of double-stranded DNA antibodies in multiple sclerosis.
… the aetiology remains largely unanswered. Anti-dsDNA antibodies have been found intrathecally and bordering lesions in multiple sclerosis patients and in view of their known pathogenity in lupus nephritis the aim of this project was to further investigate their role in multiple sclerosis. Using …
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Computational Methods for the Measurement of Protein-DNA Interactions
It is of interest to know where in the genome DNA binding proteins act in order to effect their gene regulatory function. For many sequence specific DNA binding proteins we plan to predict the location of their action by having a model of their affinity to short DNA sequences. Existing and new …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… by detecting the FRET change of dual-labeled DNA probes encapsulated in vesicles. Through our single-vesicle content mixing assay, we found the fusion pore expansion role of yeast SNAREs as well as neuronal SNAREs plus synaptotagmin 1.
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