Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 131 for “"DNA Extraction"”.
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Integrated DNA extraction and amplification on a microfluidic device
An evaluation of DNA extraction and amplification performed in microfluidic systems was carried out, with the aim of integrating the two processes in a single microfluidic device. This integrated device will then be incorporated upstream of capillary gel electrophoresis and fluorescence-based …
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Development of Innovative Technologies for DNA Extraction and Detection
… e di facile utilizzo per l'analisi del DNA (dall'estrazione alla rilevazione), implementate in dispositivi portatili e miniaturizzati. Questi tipi di tecnologie sono chiamati Point-of-Care (PoC) e potrebbero creare le condizioni per un'analisi clinica più efficiente, introducendo una …
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Magnetic bead-based DNA extraction and purification microfluidic chip
A magnetic bead-based DNA extraction and purification device has been designed to be used for extraction of the target DNA molecules from whole blood sample. Mixing and separation steps are performed using functionalised superparamagnetic beads suspended in the cell lysis buffer in a circular …
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Focused Ultrasound Extraction (FUSE) for Formalin-Fixed, Paraffin Embedded (FFPE) DNA Extraction
… and clinical applications. However, FFPE DNA extraction presents unique challenges and requires lengthy incubation periods, which can be impractical for certain applications. Here, we propose the use of focused ultrasound extraction (FUSE) technology for improved DNA extraction from FFPE …
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Sample Collection and DNA Extraction Methods for Environmental DNA Metabarcoding in Headwater Streams
<p>DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high …
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The validation of forensic DNA extraction systems to utilize soil contaminated biological evidence
Includes abstract. Includes bibliographical references.
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In situ formed magnetic ionic liquids: DNA extraction performance and fluorescence-compatibility in bioanalytical applications
<p>Extracting genomic DNA from complex biological sample matrices is often the first step in numerous molecular biology procedures such as polymerase chain reaction (PCR), cloning, and gene therapy. Obtaining high yields and pure DNA presents a significant sample preparation challenge in nucleic …
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
… Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false negative results from enzymatic detection methods that …
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Optimisation of DNA extraction from teeth submerged in sea water in False Bay, South Africa
Extracting forensically useable DNA from human remains recovered from an open marine environment is problematic, and in some cases, impossible. The reason is unclear given the lack of research on marine decomposition, DNA survival in seawater, and possible methods to optimise the DNA extraction …
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Comparison of Commercial DNA Extraction Kits with That of Organic Extraction Procedures Using Simulated Forensic Evidence Samples
<p>DNA analysis has become essential to the world of forensics in recent years. The success of such analysis requires effective methods for the extraction of DNA Two straight extraction methods: the organic phenolchloroform extraction method and the ReliaPrep<sup>TM</sup> Blood gDNA Miniprep System …
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Efficiency of Using Commercial DNA Extraction Kits and the Organic Extraction Method in Removal of Polymerase Chain Reaction Inhibitors
… determination of a perpetrator of a crime using DNA profiling is obtaining good quality DNA. The substrate on which the body fluid is located may contain substances that can co-extract with DNA and may inhibit subsequent PCR analysis. In this study, the efficiency of the removal of such …
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Optimisation of sample preparation for DNA extraction from formalin fixed paraffin embedded tissues of unresolved sudden unexpected death cases
… (FFPETs) are the only archival sources of DNA available for retrospective analyses. This study aimed to optimise DNA recovery from FFPETs for potential use in molecular autopsies of unresolved SUDY cases. To this end, DNA was extracted from FFPET sections using the QIAamp® DNA FFPE tissue …
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Human Papillomavirus DNA extraction and genotype analysis by multiplex real time polymerase chain reaction from formalin fixed paraffin wax-embedded cervical carcinoma specimens
… study aiming to extract of high quality genomic DNA from archival FFPE cervical squamous cell carcinoma cases and identify hrHPV genotypes by multiplex real time PCR (RT-PCR). Materials and methods: A retrospective search identified a total of 57 cases of cervical squamous cell carcinoma for the …
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Are Mussel Shells Environmental DNA Time Capsules? A Comparison Of Extraction Methods For Obtaining DNA From Shell Material.
… evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total …
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Effects of Anaerobic and Aerobic Digestion at Mesophilic and Thermophilic Temperatures on the Quantity of Carbapenem Resistance Genes in Wastewater Sludge
… mesophilic and thermophilic digesters, sludge DNA was isolated and purified, and target genes were quantified. The target genes included CRGs (blaGES, blaOXA-48 and blaIMP-27), 16S rRNA and Int1. Sharp declines of up to 96.9% in DNA extraction yields were observed during biological digestion. …
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Nematodes as indicators of sustainable soil health management practices in corn-soybean production
… addressed knowledge gaps by combining enhanced DNA metabarcoding tools with the standard morphological analyses to evaluate soil nematode communities under different crop management practices. The first study evaluated the use of soil DNA for profiling nematode communities. Compared to the …
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Assessment of the suitability of blood samples collected for toxicology for subsequent genetic analysis: A follow-up study after five years
… toxicological analyses could provide suitable DNA for downstream genetic analyses after an extended storage period of five years. In 2017, DNA was analysed from blood samples collected into vials containing sodium fluoride/potassium oxalate preservatives or vials without preservatives (grey and …
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Investigation and validation of mammalian telomeres as biomarkers for health and disease
… TL, with a specific focus on the effects of DNA extraction methodology, tissue-specific telomere dynamics, and telomere-specific oxidative damage. A mouse adapted monochromatic multiplex qPCR (mmqPCR) assay was developed and validated to measure relative TL, complemented by a …
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Development of a Multi-Component Biosensor for Recombinase Polymerase Amplification-CRISPR/Cas12a-Based Detection of Fusarium oxysporum f. sp. cubense tropical race 4
… for sensitive and portable detection of Foc TR4 DNA. The system combines microneedle-based DNA extraction, Recombinase Polymerase Amplification (RPA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas12a-mediated detection, and electrochemical square wave voltammetry (SWV) …
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