Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"DNA DSB"”.
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Functional Characterisation of a RECQL4 Mutation in Rothmund-Thomson Syndrome
Germline mutations affecting the RECQL4 DNA helicase cause Type II Rothmund-Thomson syndrome (RTS), a human disease characterised by defects in skeletal development and predisposition to specific types of cancer, including osteosarcoma (OS). RECQL4 has been implicated in multiple cellular functions …
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Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis
… shield against malignant transformation, DNA double-strand breaks (DSBs) are probably the most dangerous threat to the maintenance of a stable cellular genome. Mammalian cells have evolved a sophisticated DNA damage response (DDR) network that senses the physical existence of DNA DSBs, …
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Solid and hollow gold nanoparticles as radiosensitisers in combination with x-ray radiation and targeted radiopharmaceuticals
… with changes in the cell cycle, dynamics of DNA double strand break (DSB) formation and repair and apoptotic cell death was assessed. The growth of spheroids was examined by assessing changes in spheroid volume. Significant radiosensitisation was observed in UVW/NAT and SK-N-BE cells, when …
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Pre-clinical evaluation of lethal and sublethal DNA damage in human cell cultures by ultrashort pulse and ultra-high dose rate laser accelerated protons
… these beams can potentially deliver lethal DNA damaging dose which tumor cells with unstable genome may fail to repair, whilst normal cells are able to repair the same damage.<br/><br/>This Thesis work, carried on in the context of the international A-SAIL (Advanced Strategies for Accelerate …
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DNA double-strand break repair studied by atomic force microscopy
DNA double-strand breaks (DSBs), where both strands of the DNA duplex are simultaneously fractured, are considered the most lethal type of DNA damage. The conserved Mre11-Rad50 DNA repair complex enables the catalytic activities of the Mre11 nuclease and the Rad50 ATPase to function together to …
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Caretaker-Gen-Syndrome
… dieser Aberrationen wurden allerdings nur auf cDNA-Ebene detektiert. Sollte es sich hierbei in den meisten Fällen um Mutationen an den Spleiß-Konsensussequenzen handeln, läge der Anteil der Spleißmutationen im ATM-Gen beträchtlich höher (~35 Prozent) als in anderen betroffenen Genen (~15 …
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Determining molecular mechanisms of DNA Non-Homologous End Joining proteins
DNA double strand breaks (DSB), particularly those induced by ionizing radiation (IR) are complex lesions and if not repaired, these breaks can lead to genomic instability, chromosomal abnormalities and cell death. IR-induced DSB often have DNA termini modifications including thymine glycols, ring …
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Double-Strand Break Repair Pathways In Dna Structure-Induced Genetic Instability
… In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a Z-DNA or H-DNA, this can lead to genetic instability in the form of …
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Analysis of radiosensitivity in South African cervical and breast cancer patients
Introduction: Ionising radiation can cause DNA double strand breaks (DSB), that result in chromosomal aberrations if un- or mis-repaired. Individuals with compromised DNA damage repair mechanisms display increased chromosomal radiosensitivity. The G0-micronucleus assay (MN assay) and the γ-H2AX …
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Genetic strategies to manipulate meiotic recombination in Arabidopsis thaliana
… attempted to direct meiotic recombination using DNA double strand breaks targeted to specific sites. This project used a spo11-1 mutant, which is unable to produce the endogenous meiotic DNA DSBs that normally mature into crossovers. Instead, TALFokI nucleases (TALENs) were expressed from meiotic …
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Induktion und Reparatur von DNA-Doppelstrangbrüchen nach kombinierter Einwirkung von Cisplatin und Bestrahlung auf eukaryote Zellen
… wird anhand der Induktion und Reparatur von DNA-Doppelstrangbrüchen die Wirksamkeit einer kombinierten Radio- und Chemotherapie gezeigt. DNA-Doppelstrangbrüche können durch ionisierende Strahlung und sekundär durch zelluläre Reparaturmechanismen auch durch das Chemotherapeutikum Cisplatin …