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Showing 1 to 20 of 23 for “"DNA Barcode"”.
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DNA Barcode Examination of North American Mayflies Across Their Natural Distribution Reveals Cryptic Species Complexes
The application of DNA barcoding to distinguish between two or more closely related taxa has been used more frequently in recent years. The typical approach has been to isolate, amplify and sequence cytochrome c oxidase subunit 1 (COI), a mitochondrial DNA (mtDNA) encoded subunit of respiratory …
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Tracking Floral Visitation Using DNA Barcodes
… IPT, one promising alternative is the use of DNA barcoding. Because floral visitors eat, collect or unknowingly obtain pollen, nectar and other floral tissues, plant DNA should be recoverable from their bodies, permitting molecular identification of pollen loads. To assess the utility of plant …
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DNA barcoding of Jatropha curcas
<p>"DNA barcoding involves sequencing a standard region of DNA to be used as a tool for rapid species identification. The cytochrome C oxidase gene (COXI) is widely accepted as the standard barcode for animals and currently there is no barcode for land plants. The Consortium for the Barcode of Life …
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Proteome-Seq: Sequencing-Based Readout Of Proteomic Analytical Assay
… methodology termed Proteome-seq, which leverages DNA-based encoding and next generation sequencing-based readout of protein information. This approach includes two crucial pieces of protein identity information in DNA form: Antibody ID, a DNA barcode conjugates to an antibody specific to the …
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DNA barcoding e biodiversità molecolare: casi di studio nel settore ittico
… necessità. L'utilizzo di una breve sequenza di DNA per standardizzare l identificazione degli organismi ha recentemente conosciuto gli allori della cronaca sotto l intrigante termine di DNA barcoding . Un segmento di circa 650bp del gene della citocromo ossidasi I mitocondriale (COI) è stato …
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Analyzing the diet composition of Lake Trout (Salvelinus namaycush) in Upper Priest Lake
… digested fish tissue. We then extracted DNA from these tissues and used a species DNA barcode located in the cytochrome oxidase 1 gene of the mitochondrion to identify said fragments. Out of a total of 61 samples 63.4% were identified as Lake Trout; 19.0% were identified as Pygmy …
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Evaluating the Evolutionary and Genetic Relationships of the Andean Orchids of Northwestern Ecuador
<p>DNA barcoding is a molecular based technique used to separate and identify individual species. Here we establish a DNA Barcode library for the orchid flora of an Andean cloud forest in Northwestern Ecuador. The library contains 135 <em>matK</em> and 136 <em>rbcL</em> DNA Barcodes representing …
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The spread of invasive virile crayfish (Faxonius virilis) in the current river drainage of Missouri, U.S.A.: Hybridization and potential impacts on detection and management
… we wanted to develop an environmental DNA (eDNA) assay to aid in detection of F. virilis. While developing the F. virilis-specific eDNA assay, we noticed a discordance between the phenotype and mitochondrial DNA barcode of some native F. punctimanus specimens. As evidenced by a mismatch …
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Phenotypic and genotypic characterization of Thalassiosira pseudonana (Bacillariophyceae) strains
… observed. Genotypic characterization based on DNA barcode genes revealed that the strains were identical; however, Amplified Fragment Length Polymorphism (AFLP) analyses indicated that the axenic strains belonged to three different clusters. Although the population variability<br/>obtained was …
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DNA barcoding of forensically important blow flies (Diptera: Calliphoridae) within the Western Cape of South Africa
… step towards estimating post mortem interval. DNA barcoding can aid in the identification of unknown forensically relevant species, and this requires the comparison of DNA barcodes to reference data from known species. However, there is a lack of DNA barcode reference data of forensically …
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Taxonomic and Phylogenetic Diversity of Pitcher Plant Bogs in Georgia's Coastal Plain
… for floristic study and tissue samples taken for DNA barcode sequencing. A total of 290 individuals were collected (43 families; 98 genera; and 179 species), of which 254 were sequenced for rbcL and matK gene regions of the chloroplast genome to generate a phylogeny. Sequencing returned 197 …
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The Role of Nutrient Availability in Therapeutic Response of Leukemia
… under standard culture conditions, a DNA-barcode competition assay identified a subset of leukemia cell lines that grow sub-optimally under lower, more physiological thiamine levels. These cell lines are characterized by low expression of SLC19A2, a high affinity thiamine transporter. …
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Clonal deconvolution of transcriptomic signatures and their spatial organisation in a mouse model of breast cancer
… individual cells with a heritable, expressed DNA barcode coupled with high-throughput single-cell RNA-sequencing. Importantly, this lentiviral-labelling approach can be deployed in any model system that is susceptible to viral transfection. Thus, this platform allows the comprehensive and …
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DNA barcoding of forensically important flies in the Western Cape
… is damaged. Molecular identification such as DNA barcoding has therefore become a popular method of identifying species. DNA barcoding characterises species by sequencing and analysing specific regions in the genome. This technique has been used to characterise species in various countries …
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Selective Chemical Reactions for Nucleic Acid Sequencing and DNA-Encoded Library Synthesis
… is N6-methyladenine, known as 6mA in DNA and m6A in RNA. While traditional methods to sequence these modifications have depended on antibody pulldowns, chemistry-based approaches are often less sequence dependent, can work on either DNA or RNA, and thus can provide a robust, …
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Applications of DNA-barcoding in the identification and understanding of grass invasions in Southern Africa
… These grasses can be difficult to identify. DNA barcoding has been suggested as an alternative method of identifying grasses in the hope of facilitating identification of existing invaders and preventing future invasions. In this study a list of all known naturalised grasses in South Africa …
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Testing the utility of the consortium for the barcoding of life's two 'agreed upon' plant DNA barcodes, matK and rbcL
DNA barcoding is the use of short standardized regions of DNA to identify unknown specimens to species. Currently, the zoological community has agreed that cytochrome oxidase I subunit 1 (COI), a mitochondrial gene region, will serve as the barcode region for all animal taxa. Due to oftentimes …
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Impiego di tecniche innovative per la valorizzazione e caratterizzazione molecolare di specie vegetali ornamentali
… quello di valutare la capacità dell approccio DNA barcoding per identificare diversi gruppi tassonomici da due collezioni di piante da fiore: taxa commerciali più rilevanti, provenienti dalla produzione in vivaio e piante autoctone mediterranee con atteggiamento ornamentale per nuova …
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An in vitro chemogenomic screening system to identify the molecular targets of antimalarial drug candidates
… pathways) were engineered to possess a unique DNA barcode. These barcodes can be used to detect and quantify constructs within complex pools using a next generation sequencing (NGS) process termed barcode sequencing (BarSeq), allowing screening of multiple lines in parallel. I transfected PbACs …
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Phylogenetic Analyses of Andean and Amazonian Tree Communities in Ecuador
… of this research was to evaluate the magnitude ofDNA barcode diversity among Amazonian and Andean tree species. Specifically, the objectives were to (1) evaluate community phylogenetic structure and correlate phylogenetic analyses with diversity metrics among Andean tree species along an …
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