Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"DNA, Single-Stranded"”.
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
DNA replication is a complex process involving many proteins that must be performed for a cell to proliferate. In this work, we will study three members of the replication system: single-stranded DNA, single-stranded DNA binding proteins, and the sliding clamp and clamp loader proteins. …
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Defining the Role of Secondary DNA Structures and Transcription Factors on the Transcriptional Control of the HIF-1alpha and VEGF Promoters
… undergo a conformational transition between B-DNA, single stranded DNA and atypical secondary DNA structures such as G-quadruplexes and i-motifs. We hypothesize that the i-motif and G-quadruplex structures can form within the pPu/pPy tracts of the HIF-1α and VEGF proximal promoters, which play …
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Development of a T-Loop Assay to Investigate T-Loop Dynamics and Structure
… to the difficulty of isolating and visualizing DNA with intact loop structures. Thus, we have developed novel methods to isolate DNA such that biochemical assays and microscopic visualizations for authentic t-loops are now possible. Digestion of proteins that stabilize t-loop and significant …
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Assay for Rifampicin Resistant M13 RF Synthesis
… of the origin of 0X174 complementary strand DNA in Ml3 by virtue of its ability to initiate the synthesis of M13 complementary strands in the presence of the antibiotic rifampicin. A segment of DNA carrying the sequence recognized by the replication protein n' has been isolated from …
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Restriction Enzyme Analysis of Incomplete Parental Replicative Forms of øX174
… initial step in the infectious cycle of the single-stranded DNA phage øX174 is the synthesis of a DNA strand complementary to the infecting viral DNA producing a duplex molecule, the parental replicative form (RF). Electron micrographic examination of parental RF produced from viruses exposed …
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Distinct functions of POT1 at telomeres.
… the end of the chromosome from sites of DNA damage and control cellular replicative potential. The loss of function of telomeres results in several biological consequences. First, dysfunctional telomeres elicit DNA damage responses and repair activities, which frequently induce …