Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"DMEM"”.
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Un Macro Elemento Discreto co-rotazionale (DMEM) per l’analisi in grandi spostamenti di strutture in muratura.
… (DEM). Questa tesi si basa su un approccio DMEM (Discrete Macro-Element Method) recentemente proposto con l'obiettivo di cogliere il comportamento non lineare di un'intera struttura attraverso un assemblaggio di macro-elementi discreti caratterizzati da diversi livelli di complessità in …
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Eviscerated corneas as tissue source for ex vivo expansion of limbal epithelial cells on platelet-rich plasma gels
… following trauma using A-PRP combined with DMEM (Dulbecco's modified Eagles medium), versus DMEM alone. Materials and Methods: This was a laboratory case controlled study of human corneal cells cultured in a mixture of A-PRP and DMEM, versus DMEM alone from 6 eviscerated corneas. A hundred …
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Biochemical and Immunocytochemical Characterization of Canine Corneal Cells Cultured in Two Different Media
… to Dulbecco's modification of Eagle's medium (DMEM) with fetal bovine serum (FBS), and to characterize cultured canine corneal cells. Superficial keratectomies were performed on three dogs. Samples were trypsinized to separate cell layers. Post-trypsinization, immunohistochemistry confirmed …
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Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
… and dilute Cytochalasin-B in culture media (CB-DMEM, 30.0 μM) with 0.05 % ethanol solutions (diluent) to produce four different concentrations of CB-DMEM (5.3, 10.6, 14.8, and 20.2 μM). The different concentrations of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking …
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<em>In Vitro Cultivation</em> of the Microphallid Trematode <em>Gynaecotyla Adunca</em>
… and the number of eggs deposited, the media DMEM and RPMI-1640 were compared to Hank's Balanced Salt Solution. Different sera were also tested including horse, new-born calf, and chicken, the best of which was tested at different concentrations. To test the viability of the eggs deposited by …
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Effect of Postmortem Time and Preservation Fluid on the Tensile Material Properties of Bovine Liver Parenchyma
… of bovine liver parenchyma when stored in DMEM or saline. Fourteen fresh bovine livers were obtained from a local slaughter house and stored in either DMEM or saline as large blocks, small blocks, or slices of tissue. Multiple parenchyma dog-bone samples from each liver were tested once to …
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Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering
… 2[mu]g), the post-transfection medium (Medium 1: DMEM low glucose, 20% FBS and 1% antibiotic versus Medium 2: DMEM low glucose, 20% FBS, 1% antibiotic and 10ng/ml FGF-2) and the culture environment during transfection (static versus dynamic). The objective of the second part was to determine the …
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Physiological Oxygen and Media Conditions Influence Breast Cancer Hallmarks in Vitro
… media such as Dulbecco’s Modified Eagle Medium (DMEM) contain supraphysiological levels of key metabolites like glucose while lacking others entirely. Additionally, standard cell culture incubators maintain near-atmospheric oxygen levels (~18% O2), far exceeding the physiological oxygen levels …
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Regulation of Endothelial Nitric Oxide Synthase in Pulmonary Myofibroblasts
… The cells were grown in normal growth media (DMEM + 10% FBS) or serum starved (DMEM + 0% FBS) to induce cellular differentiation. In this study, immunocytochemistry was used to show localization of eNOS is dependent on cellular differentiation, with protomyofibroblasts expressing eNOS …
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The Effect of Endothelin-1 on the expression of CDK Inhibitors p21 & p27 in Bovine Corneal Endothelial Cells
… isolated from bovine corneas and cultured in DMEM supplemented with 10% serum. 5-Bromo 2-deoxy Uridine (BrdU) incorporation was determined in serum starved cultures in 24-well plates as a measure of cell proliferation. Confluent serum starved cells grown in T-25 flasks were treated with 100nM …
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Human Organotypic Retinal Cultures as a model of human retinal ganglion cell degeneration in glaucoma. Effects of epigenetic regulation and mesenchymal stem cell derived growth factors.
… cell death in HORCs. Results: Serum-free (SF) DMEM/HamF12 with no added antibiotics was found to be the medium of choice for the human retina culture. A statistically significant loss of NeuN-labelled RGCs was documented after 2 weeks in culture using SF DMEM/Ham F12, whereas with Neurobasal …
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Osteochondral allograft preservation in a serum-free chemically-defined media
… evaluate OCAs stored up to 56 days in a Control (DMEM supplemented with ITS, Sodium Pyruvate, L-ascorbic acid, and MEM Non-essential amino acids) and Test (DMEM) supplemented ITS+, Sodium Pyruvate, L-ascorbic acid, MEM Non-Essential amino acids, dexamethasone, boron, and TGF-[beta]3) media …
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Analysis of cellular responses to microwave irradiation in E. coli and change of oxygen level and culture medium in human cancer cell lines using RNA-seq based transcriptomic profiling
… changes when compared to PC3, especially in DMEM at 18% O2. DNA replication, cell-cycle, and viral carcinogenesis are the most affected pathways observed from different culture conditions in both cell lines. In PC3, only the legionellosis seems to be most impacted by culture medium changes at …
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The Isolation and Characterization of Nannobacteria
… to culture nannobacteria on TSA, R2A, SDA, DMEM and RPMI in environments of 25°C, 37°C, 37°C with CO2, and 45°C, have been made. Experiments to rule out the possibility that they are biological artifacts, such as bacterial fragments have also been performed.
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The Effects of Ketoconazole on the Oxidation of Low Density Lipoproteins by Bovine Aortic Smooth Muscle Cells
… Confluent BASMC were fed 1 ml of F- 12/DMEM containing LDL (320 ug protein). The cells over a 24 hour period caused a 2x increase in LDL oxidation as measured by an increase in thiobarbituric acid reactive substances. Copper at 5 uM enhanced by 2x the oxidation of LDL by BASMC. Oxidized …
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Development of exosome-based anti-cancer therapeutics
… and M2 using dulbecco's modified eagle medium (DMEM), where M1 supplemented with 10 % (v/v) FBS, and M2 with 10 % (v/v) exosome depleted FBS (Dep-FBS). However, after cells reached confluency, those two media were removed and replaced with serum free media (only DMEM), to create M3 and M4, …
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Activity and mRNA abundance of enzymes for fatty acid synthesis and desaturation in mammary cell cultures
… in Dulbecco's modified Eagle's medium (DMEM). As indicated by cellular palmitic acid (16:0) content and fatty acid synthetase (FAS) activity, when compared with SA all unsaturated fatty acid treatments inhibited de novo fatty acid synthesis in MME cells. In addition, OA at all …
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The Effects of Host-like Environmental Signals and Gene Expression on Capsule Growth in Cryptococcus neoformans
… and capsule size. These signals are medium (YPD, DMEM or RPMI), temperature (30°C or 37°C), CO<sub>2</sub> (room air or 5%), cAMP (0 mM or 20 mM), and pH buffer (HEPES/no HEPES). We explore the effects of exogenous cAMP at a range of concentrations and of deletions of cAMP pathway genes …
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Pengaruh ekstrak pegagan (Centella asiatica linn.) terhadap pertumbuhan kultur primer sel hepar baby hamster yang dipapar 7.12- dimetilbenz(α)antrasen
… µg/mL, 4000 µg/mL yang ditumbuhkan dalam medium DMEM 10% FBS. Setelah masa inkubasi, kultur primer sel hepar baby hamster diamati konfluen, viabilitas, dan sitotoksisitas sel. Hasil penelitian menunjukkan bahwa ekstrak pegagan mampu menurunkan konfluen sel, viabilitas sel dan mengakibatkan …
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Pengaruh pemberian ekstrak etanol daun sirsak (Annona muricata L.) terhadap pertumbuhan sel neuroglia baby hamster yang dipapar dengan 7, 12-Dimetilbenz (α) Antrasen (DMBA) dalam kondisi in vitro
… dua hari yang ditumbuhkan dalam medium dasar DMEM (Dulbecco’s Modified Eagle’s Medium) dengan dan tanpa dimetilbenz (α) antrasen (DMBA). Konsentrasi ekstrak etanol daun sirsak yang digunakan adalah 0 µg/ml, 10 µg/ml, 20 µg/ml, 40 µg/ml, 80 µg/ml dan 160 µg/ml. Parameter dalam penelitian ini …
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