Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"DIGE"”.
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Use of comparative proteomics to study a novel osteogenic nanotopography
… fluorescence difference gel electrophoresis (2D DIGE) is a new method for assessing protein expression strategies and here, a micro-grooved topography was used as a model for protocol optimization. The protocol was successfully developed and proved that 2D DIGE can be used as a powerful tool in …
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Global and targeted proteomics in Arabidopsis thaliana: A study of secondary metabolism and phytohormone signaling
… the methodology to apply traditional and DiGE-based 2D-GE strategies to global proteomics in Arabidopsis. Our results suggest that while 2D-GE is applicable to Arabidopsis, there are practical and conceptual limitations that must be understood. Further, our results suggest that …
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
… of both samples as an internal standard. Using DIGE technology, the statistically significant comparisons of each protein abundance was made over three biological replicates. 43 protein spots were identified as differentially regulated. Among the 43 protein spots, 25 were found to be …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
… virulence. Differential in-gel electrophoresis (DIGE) showed that ansB and ggt mutations exert pleiotropic effects on the expression of a number of S. flexneri genes, including prominent bacterial outer membrane proteins, OmpA and YaeT. This is the first report in S. flexneri where the functions …
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Characterization of novel virulence factors of Shigella flexneri and development of Caenorhabditis elegans as an animal model for Shigella infection
… virulence. Differential in-gel electrophoresis (DIGE) showed that ansB and ggt mutations exert pleiotropic effects on the expression of a number of S. flexneri genes, including prominent bacterial outer membrane proteins, OmpA and YaeT. This is the first report in S. flexneri where the functions …
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Proteomic profiling of skeletal muscle tissue from the Goto-Kakizaki rat model of type 2 diabetes.
… fluorescence difference in-gel electrophoresis (DIGE) and electrospray ionization liquid chromatography mass spectrometry (ESI LC/MS) were employed to analysis the diabetic muscle tissue proteome and sub-proteome. A differential expression pattern was observed for proteins involved in glycolysis, …
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Development of a chemical biology approach for glycoprotein discovery in Caenorhabditis elegans reveals glycosylated isoforms of multiple proteins with mitochondrial function
… differential gel electrophoresis (DIGE). In this approach, proteins in different samples are metabolically labeled with azido-substrate analogs, the azido-labeled proteins are marked with custom-synthesized Cy3- or Cy5-alkyne probes via Click Chemistry, and then differences in the …
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Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii
… difference in gel electrophoresis (2D-DIGE) was used to compare the soluble proteome of one low CO2-adapted pyrenoid-less RBCS mutant compared to the pyrenoid-positive wild-type. This analysis identified only a few differentially expressed proteins, none of which were directly involved …
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Telomerase a prognostic marker an therapeutic target
… A comprehensive proteomic study utilizing 2D-DIGE and MALDI-TOF were used to assess the effect of inhibiting two different regulatory mechanisms of telomerase in glioma. RNAi was used to target hTERT and Hsp90α. Inhibition of telomerase activity resulted in down regulation of various …
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Functional genomics of a non-toxic Alexandrium lusitanicum culture
… derivatives. Difference gel electrophoresis (DIGE) identified proteins differentially expressed between the two subcultures. Identification of some of these proteins was possible by searching the expressed sequence tag (EST) database for dinoflagellates. Proteins shown to be down-regulated in …
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Etude protéomique de l'incidence d'une hypertriglycéridémie sur la mitochondrie de foie de souris
… caractérisé, par une analyse protéomique en 2D-DIGE, les adaptations du métabolisme mitochondrial hépatique à une hypertriglycéridémie modérée et sévère chez la souris HuApoC-III afin d’établir la dépendance de la réponse métabolique vis-à-vis du niveau d’hypertriglycéridémie. Cette analyse a …
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Rheumatoide Arthritis: Proteomische Analyse von Serum und synovialen Fibroblasten zur Detektion von Biomarkern
… durch Differenzielle-In-Gel-Elektrophorese (2D-DIGE) untersucht. Zuvor wurden high abundand Proteine durch den Einsatz einer MARC (multiple affinity removal column) abgereichert, um die low abundand Proteine besser visualisieren zu können. Dabei konnte in den Seren von RA-Patienten das Serum …
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Molecular mechanisms of drug resistance, invasion and metastasis in pancreatic carcinoma
… comparative proteomic profiling by 2-D DIGE, MALDI-TOF MS and RNAi revealed three targets, ALDH1A1, VIM and STIP1 with functional involvement in the invasion process. ALDH1A1 appeared to play a role in the aggressive invasive phenotype; expression was associated with increased invasion, …
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Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome
… difference in-gel electrophoresis (2D-DIGE) in vivo after 48 h in hypoxia vs. normoxia. The results showed that proteins involved in mitochondrial oxidative metabolism are down-regulated, whereas glycolytic enzymes are up-regulated to compensate for the loss of ATP synthesis in aerobic …
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Regulation of gene expression and adhesion in <i>Saccharomyces cerevisiae</i>
… Bedingungen zu identifizieren, wurden 2-D-DIGE-Experimente mit ∑1278b Hefezellen durchgeführt. Sieben Proteinpunkte zeigten eine stark erhöhte Intensität unter Aminosäuremangel. Diese Proteinpunkte wurden durch Massenspektrometrie als Cpc2p, Efb1p, His1p, Hsp60p, Sod1p, Tpi1p und Tpm1p …
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Emerging anabolic drugs - investigation of the in vitro and in vivo metabolism of selective androgen receptor modulators
… difference gel electrophoresis (2-D DIGE) to identify differential protein levels with subsequent matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis to cause a response to the hepatic proteome. Subsequent differential centrifugation of …
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Characterisation of novel and complex mechanisms of antobiotic resistance using a proteomics approach
… These included the use of 2-D electrophoresis, DIGE and LC-MS/MS mass spectrometry applied to multidrug resistant <i>Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Serratia marcescens</i> and <i>Acinetobacter baumannii</i>. In summary, these investigations revealed that the …
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Gene and Protein Expression in Human Gliomas
… and 2D differential in-gel electrophoresis (DIGE) with sequential tandem mass spectrometry (MS/MS) protein identification, to investigate the gene and protein expression profiles in a set of gliomas of varying grades, as well as in a number of normal human brain samples. We found that these …
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Untersuchungen zur Pathogenese der dilatativen Kardiomyopathie in Cathepsin L-defizienten Mäusen
… Analysen, wie der in dieser Arbeit begonnenen DIGE-Untersuchung erbracht werden.
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