Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 74 for “"DGGE"”.
-
Using Hplc and Pcr-Dgge for the Assessment of Phytoplankton and Bacterioplankton Communities in Scottish Freshwater Lakes
… and denaturing gradient gel electrophoresis (PCR-DGGE) for the assessment of the plankton community in Scottish freshwater lakes. Phytoplankton composition and biomass were determined using microscopy and HPLC, while bacterioplankton density and diversity were assessed using flow cytometry (FC), …
-
Molecular microbial ecology of hospital ward environments.
… the presence of resistance determinants. PCR-DGGE was used to resolve fragments of the 16S rRNA gene from total DNA extractions and the resulting sequence data was used to identify microorganisms present. Cell viability was assessed using RNA as the template for reverse transcriptase PCR-DGGE. …
-
Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… to use denaturing gradient gel electrophoresis (DGGE) to separate the PCR products before DNA sequencing. Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on their melting point, which is determined by their DNA sequence. This analytical technique allows a mixture of …
-
Detection of an Introduced Bacterial Culture in Greywater Treatment Reactors
… and denaturing gradient gel electrophoresis (DGGE) revealed community profiles of the dominant bacterial species. Species and strainspecific primers targeting either the 16S rRNA gene or the intergemc spacer (ITS) between the 16S and 23S rRNA genes were developed to detect the inoculum strain …
-
Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
… such as 16S rDNA library construction and DGGE have allowed for increased access and better understanding of cyanobacterial diversity from diverse biotopes. In this investigation, 16s rDNA analysis and DGGE profiling were used to study the diversity of cyanobacteria along a transect of …
-
Improving Our Comprehension of Microbial Communities
… such as denaturing gradient gel electrophoresis (DGGE), have been used as an alternative to SSU rRNA gene sequencing because they can provide many community profiles for a fraction of the time, effort and money required for sequencing. Chapter 3 provides a comparison of DGGE community profiles and …
-
Characterization of prokaryotic diversity in a chlorinated solvent-contaminated bedrock aquifer using denaturing gradient gel electrophoresis, ribosomal DNA sequencing, and real time PCR
… use of denaturing gradient gel electrophoresis (DGGE), and were analyzed to detect spatial and seasonal changes. DGGE fingerprints revealed significant compositional differences between the attached and the unattached microbial communities. Cluster analysis suggested that the key factor …
-
A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil
… and denaturing gradient gel electrophoresis (PCR-DGGE) procedure for the detection of fungal 18S ribosomal RNA gene was developed with reference cultures. Five AMF species were procured from the International Culture Collection of Arbuscular and Vesicular-Arbuscular Mycorrhizal Fungi (INVAM). …
-
To study the fitness of Bacillus thuringiensis to survive in natural and artificial, terrestrial ecosystems
… cereus/B. thuringiensis. The three techniques of DGGE, T-RFLP and MLST were compared for the first time to judge whether the fingerprinting and typing techniques provide the same level of resolution in the structure of the bacterial communities. The techniques resulted in interesting findings: (a) …
-
Rhizosphere/Soil Microorganisms in the Phytoremediation of Biphenyl and Dioxane
… The denaturing gradient gel electrophoresis (DGGE) results indicated that soil type had a significant impact on microbial community composition with a more diverse microbial population in native soil samples than in engineered soil samples. Although tree type had less influence on microbial …
-
The effect of chaotropic magnesium chloride on the growth of microbes
… marsh fungi dominated in MgCl₂ enrichments and DGGE analysis of enrichments revealed high community diversity for Bacteria and Archaea but low community diversity for fungi. Sequencing of selected DGGE bands showed the presence of an Acremonium-related species in MgCl₂ at 1.5 M and Baeospora …
-
Gut-associated microbial symbionts of the marsh fiddler crab, Uca pugnax
… by denaturing gradient gel electrophoresis (DGGE). Community composition, however, does differ between stomach and hindgut populations, as resolved by DGGE and clone libraries of the 16S rRNA gene. Many recovered clones were most closely related to other symbiotic or gut-associated bacteria. …
-
A multi-disciplinary approach to the characterization of waterlogged burial environments : assessing the potential for the in situ preservation of organic archaeological remains
… community structure was examined by PCR-DGGE.Redox potential readings were positive above the water table and negative below. The bacterial abundance and activity were greatest at the soil surface and, in general, decreased with depth. BIOLOG showed both depth variation and between site …
-
In vitro examination of the effect of orlistat on the ability of the faecal microbiota to utilize dietary lipids
… and denaturing gradient gel electrophoresis (DGGE) analyses], but did show there was great variability in the lipolytic activities of the microbiotas of individuals, as determined by gas chromatography analysis of long-chain fatty acids in samples. Subsequent studies focused on the effect of …
-
In vitro examination of the effect of orlistat on the ability of the faecal microbiota to utilize dietary lipids
… and denaturing gradient gel electrophoresis (DGGE) analyses], but did show there was great variability in the lipolytic activities of the microbiotas of individuals, as determined by gas chromatography analysis of long-chain fatty acids in samples. Subsequent studies focused on the effect of …
-
Veränderungen in der Zusammensetzung von Bakterioplankton-Gemeinschaften durch Inkubation mit den Süßwasser Makrophyten Iris pseudacorus und Mentha aquatica
… 16S rDNS und statistische Auswertung der DGGE-Bandenprofile. Im Vergleich zu Gemeinschaftsprofilen unbehandelter Teichwasserproben kam es bei Bakteriengemeinschaften in Pflanzen-Inkubationsansätzen zu Neubildungen und Deletionen. Während Neubildungen auf Pflanzen-assoziierte Bakterien …
-
Caracterização da microbiota lática do Queijo Minas artesanal produzido na região do Serro, Minas Gerais, por métodos cultura dependente e independente
… Rep-PCR, Denaturing Gradient Gel Eletrophoresis (DGGE) e sequenciamento da região V3-V4 do 16S rRNA. A contagem de BAL em amostras e QMA do Serro variaram entre 5,4 e 8,4 log UFC/g com um valor médio de 6,9 log UFC/g, e os principais grupos/gêneros identificados foram lactobacilos, Lactococcus …
-
Assessment of flagellate diversity at deep-sea hydrothermal vents using the combined approach of culture-dependent and culture-independent methods
… by denaturing gradient gel electrophoresis (DGGE) using PCR primers specific to the kinetoplastid clade. By comparing these two different methods of analysis, my goal was to decrease the biases and/or errors inherent in either method alone and to improve our ability to assess flagellate …
-
Struktur und Dynamik heterotropher Bakteriengemeinschaften im Wattenmeer und der Deutschen Bucht
… wurden mittels gruppenspezifischer DGGE Korrelationen zwischen dem Auftreten der Kieselalge Guinardia delicatula und den Bakteriengruppen Bacteroidetes und Roseobacter gefunden. Im Tagesgang fand sich trotz Änderungen bakterieller Abundanz und -Aktivität keine systematische …
-
Diversität und Dynamik von Bakteriengemeinschaften in vier ausgewählten Seen der Mecklenburgischen Seenplatte
… Denaturierender Gradienten Gelelektrophorese (DGGE) und Klonbibliotheken von 16S rRNA-Genen wurde die phylogenetische Diversität und Saisonalität frei-lebender und partikel-assoziierter Bakterien untersucht. Die Analysen erbrachten signifikante Unterschiede zwischen den Bakteriengemeinschaften …
Page 1 of 4