Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"DGCR8"”.
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Understanding Recognition of Primary MicroRNAs by Human Drosha/DGCR8
… Drosha, in complex with its partner protein DGCR8, must distinguish its RNA substrates (called primary microRNAs) and identify the proper positions within these substrates to perform ribonuclease activity. My thesis work focuses on how the Drosha/DGCR8 complex recognizes primary microRNA …
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Δnp63 Regulates A Complex Network of Target Genes In Limb and Epidermal Development
… This phenotype is reminiscent of the DGCR8fl/fl;K14Cre and Dicerfl/fl;K14Cre mice skin. Importantly, DGCR8-/- embryonic stem cells (ESCs) display a hyperproliferation defect by failure to silence pluripotency genes. Furthermore, I have observed that epidermal cells lacking ΔNp63 …
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Investigating The Roles of The P63 Isoforms In The Microrna Biogenesis Pathway
… <em>ΔNp63</em> transcriptionally regulates <em>DGCR8</em>. <em>TAp63<sup> -/-</sup></em> mice are highly tumor prone. These mice develop metastatic mammary adenocarcinomas, squamous cell carcinomas, and lung adenocarcinomas to distant sites including the liver, lungs, and brain. I found that …
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The Regulation of Microrna Biogenesis By Ribosome-Interacting Proteins
… to as pri-miRNA) which are cleaved by Drosha and DGCR8 to generate 60-90 nucleotide precursor miRNA. The precursors are cleaved again by Dicer and loaded into the RNA-induced silencing complex (RISC) of which Argonaute 2 is the functional component. Many of the proteins involved in miRNA …
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… filed. Recent studies suggest that the Drosha-DGCR8 complex can be recruited to chromatin to catalyze co-transcriptional processing of primary microRNAs (pri-miRNAs) in mammalian cells. However, the molecular mechanism of co-transcriptional miRNA processing is poorly understood. Here, we find …
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Sequence determinants of pri-miRNA processing
… an RNase III enzyme, and an RNA-binding partner DGCR8. However, it is unclear how the Microprocessor distinguishes between these hairpins and authentic pri-miRNAs. In fact, C. elegans pri-miRNAs are not processed in human cells, illustrating the complexity of pri-miRNA recognition and processing. …
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Investigation of Copy Number Variation in South African Patients with Congenital Heart Defects
… likely to play a role in the development of CHD (DGCR8, JARID2, KDM2A, and FSTL1). The CNVs were identified in nine unrelated individuals: five of the CNVs were classified as pathogenic or likely pathogenic (5.6% of the cohort) and four were classified as variants of unknown significance (4.6%). …
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The Roles of miR-155 and miR-15/16 in Natural Killer Cell Function and Maturation
… that are subsequently 'cropped' by the Drosha/Dgcr8 complex into precursor miRNAs (pre-miRNA) that have a characteristic stem-loop structure. The pre-miRNA is exported to the cytoplasm where it is further processed by Dicer1, yielding a mature 18-24 nucleotide miRNA. The mature miRNA is loaded …