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Showing 1 to 11 of 11 for “"DEAE cellulose chromatography"”.
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Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
… filtration, ammonium sulfate fractionation and DEAE-cellulose chromatography. The histone methyltransferases were resolved into two distinct fractions by Sepharose 6B-100 and DEAE-cellulose chromatography. One enzyme fractionated by DEAE-cellulose chromatograpy was specific for histone H3, while …
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Cholesterol metabolism in the endoplasmic reticulum of rat liver
… choice of solubilising agent for this purpose. DEAE-cellulose chromatography of microsomal protein solubilised with Nonidet P42 was confirmed to be a suitable first step in the purification of liver microsomal cytochrome P-450, since there is a good separation of this species from NADPH …
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Biosynthesis of folate polyglutamates in the rat
… folate at 24 hours after administration. DEAE-cellulose chromatography revealed three principal folate polyglutamates; folate polyglutamates A, B and C. Preliminary identification indicated these to be 10-formylfolic acid tetraglutamate (10CHOFA(glu) ,), …
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Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
… the remaining cytoplasmic pool was purified by DEAE-cellulose chromatography. The HS in all pools from the confluent cells exhibited a higher average charge density than the corresponding pools from the growing cells. Analysis of the mixtures of di- and tetrasaccharides formed by cleavage of HS …
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Competitive Enzyme Immunoassay by Polyclonal Antibodies Against Organophosphorus Pesticides
… for monitoring OPs in water and vegetables. Gas chromatography (GC) is the usual method for OPs screening, but it is time-consuming and has the high cost of instrumentation. The goal of this project was to find an easy method which was sensitive and had less instrumentation required to test …
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Ribonucleotide reductase in dividing cells: Purification and inhibition studies with 4-hydroxynonenal
… rat liver. The enzyme was purified 30 fold after DEAE-cellulose chromatography. The CDP reductase activity in tissues with different growth states or rates was compared. The enzyme activity was developed well in juvenile rat liver, regenerating liver and hepatoma (cells), while the enzyme activity …
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Tumor-induced immunosuppression: Contribution of a high molecular weight inhibitor and Prostaglandin E2
… apparently >67 kd, as shown by S-200 Sephacryl chromatography and gel electrophoresis. DEAE-Cellulose chromatography suggested that the pl of the inhibitory factor was < 7.7. lsoelectric focusing revealed that the Mφ-mediated inhibitory activity differed in charge, with a pl of 6.5-7.6 for …
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Non-lysosomal protein degrading systems in chicken skeletal muscle
… extracts (fraction II: depleted of ubiquitin by DEAE-cellulose chromatography) with 125I-ubiquitin and Mg2+-ATP, followed by analysis of the radiolabelled conjugates by one-dimensional polyacrylamide gel electrophoresis in the presence of SOS, and autoradiography. Discrete conjugates were formed …
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Non-lysosomal protein degrading systems in chicken skeleton muscle
… extracts (fraction II: depleted of ubiquitin by DEAE-cellulose chromatography) with 125rubiquitin and Mg2+-ATP, followed by analysis of the radiolabelled conjugates by one-dimensional polyacrylamide gel electrophoresis in the presence of sos, and autoradiography. Discrete conjugates were formed …
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The role of topoisomerase II in amsacrine resistance
… cell cytoplasmic extracts by salt precipitation, DEAE-cellulose chromatography and SDS-PAGE resulted in a 3571-fold purification of the PDC enhancing activity. The PDC enhancing activity was shown to reside in a 70 kDa protein kinase with specificity for a casein kinase II substrate and sensitive …
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Comparison of the Protein Contents of Bovine Zymogen Granules and of Pancreatic Juice
… the pancreatic juice have been fractionated by chromatography on DEAE cellulose and IRC-50 by the procedures employed by Keller, Cohen and Neurath. Trypsinogen, chymotrypsinogen A, ribonuclease, amylase, chymotrypsinogen B, procarboxypeptidase B, deoxyribonuclease and procarboxypeptidase A have …