Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"DEAD-box"”.
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Kristallstrukturanalyse des spleißosomalen DEAD-Box Proteins hPrp28
DEAD-Box Proteine gehören zur Superfamilie der Helikasen. Sie verwenden die Energie aus der ATP-Hydrolyse, um RNA-RNA- oder RNA-Protein-Interaktionen aufzulösen und katalysieren auf diese Weise Konformationsänderungen in Ribonukleoproteinkomplexen. Das DEAD-Box Protein hPrp28 ist Bestandteil des U5 …
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Local and global investigations into DEAD-box protein function
… and foster folding to functional conformations. DEAD-box proteins are ATP-dependent RNA chaperone proteins that function by disrupting structure, which can facilitate structural conversions. Here, studies with both local and global focuses are used to uncover mechanistic features of DEAD-box …
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Investigating the cellular localisation of DEAD box helicase DDX3
DDX3 is an RNA helicase that has been shown to have a range of nuclear and cytoplasmic functions, including transcription, translation, splicing and mRNA export. DDX3 has also been shown to play a role in innate immune signalling and tumorigenesis. DDX3 is targeted by multiple viruses: HIV, HCV , …
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Genetic and biochemical analysis of a conserved DEAD-box helicase motif
DEAD-box proteins are non-processive helicases that use ATP to power unwinding of short RNA duplexes in essential steps of RNA metabolism. Despite acting on a diverse range of RNA substrates, DEAD-box proteins share a helicase core defined by 13 highly conserved amino acid motifs. Here we use S. …
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CHARACTERIZATION OF THE FUNCTION OF THE DEAD-BOX RNA HELICASE DBP2
… formation are critical for gene expression. DEAD-box proteins are the largest class of RNA helicases that play fundamental roles in RNA and RNP structure remodeling. However, the precise biological role of the vast majority of the ~ 40 members in this family has not been completely described. …
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The role of DEAD-box helicase 1 in humoral immune responses
… event, in which the RNA-binding protein DEAD-box helicase 1 (DDX1) is implicated in targeting the mutagenic enzyme Activation-Induced Cytidine Deaminase (AID) to Ig loci to initiate CSR. The aim of this project was to interrogate further the role of DDX1 during humoral immune responses, …
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The Effects of DEAD-box Helicase Activity on Biomolecular Condensate Structure and Dynamics
… in active materials. In this work, we study how DEAD-box helicases dictate condensate structure and dynamics via their enzyme-dependent RNA interactions and RNA remodeling activity. First, we show how a DEAD-box helicase’s nucleotide dependent RNA-binding dynamics can give rise to new effective …
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The role of human DEAD-box protein DDX3X in the activation and function of Estrogen Receptor-alpha
The human DEAD-box RNA helicase DDX3X was shown to be involved in innate immune signalling pathways mediated by IKKε and TBK1. Interestingly, both DDX3 and IKKɛ have independently been shown to act as breast cancer oncogenes. In breast cancer, DDX3 expression was shown to be upregulated and linked …
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The role of the DEAD box Helicase DDX3X in Anti-‐Viral Signalling Pathways Mediated by the Kinase IKKα.
The human DEAD-box RNA helicase DDX3X has previously been shown to be involved in innate immune signalling in pathways mediated by IKKε and TBK1. In this work we show that DDX3X is also involved in pathways independent of these kinases, and functions by interacting with two related kinases IKKα and …
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Using S. pombe to Study the Biological Roles of the Histone Deacetylases Sir2 and Clr3 and the DEAD-Box RNA Helicase Ded1
… formation and cancer associated mutations of the DEAD-box RNA helicase DDX3X, homolog of fission yeast Ded1, in translational control. Heterochromatin in fission yeast is characterized by hypoacetylation as well as methylation of histone H3 on lysine 9 (H3K9me). Heterochromatin assembly can now be …
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Identification of hepatitis C virus core protein residues critical for the interaction with the cellular DEAD-Box Helicase DDX3 and their functional relevance
… protein shown to interact with HCV core is the DEAD-box RNA helicase DDX3. In cells expressing either HCV core alone, or as part of the full length HCV polyprotein, DDX3 is redistributed from its normal diffuse cytoplasmic localisation to lipid droplets where it colocalises with core. The …
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The Role of Coregulator Proteins in Nuclear Receptor Transcriptional Activity
… studies in which I have characterized a novel DEAD box RNA helicase as a nuclear receptor coregulator. I have also examined the similarities in the actions of ERalpha and ERbeta using large-scale chromatin arrays and ER subtype-selective ligands.
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Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
… include the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the glycolytic enzyme enolase, and the phosphorolytic exoribonuclease PNPase. The degradosome has also been previously shown to associate with the RNA chaperone Hfq to form a small RNA guided machinery that targets …
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HAGE, a novel cancer/testis antigen with strong potential as a target for immunotherapy against cancers
… has been previously described as member of the DEAD-box family of ATP-dependent RNA helicases but very little is known about its actual function. RNA helicases are involved in various steps of RNA metabolism and their over-expression has often been linked with tumorogenesis. Using a combination …
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Approach to the identification of sex-determining genes in the tilapia genome by genetic mapping and comparative positional cloning
… Fugu, tilapia, and human. A few genes such as a DEAD box protein, Sox family, and a LIM/homeobox, seem to be good candidates for sex-determining genes, and will need further study. AFLP (amplified fragment length polymorphism)/BSA (Bulked segregant analysis) technique was performed to add more …
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Funktionelle Analyse des Transkriptionsfaktors TGA2.1 aus Nicotiana tabacum: Identifikation von Interaktionspartnern und Charakterisierung transgener Pflanzen mit reduzierter TGA2.1-Menge
… sensor systems durchgemustert. Es konnte eine DEAD-box RNA-Helicase und eine E2-Ligase als Interaktionspartner identifiziert werden. Zusätzlich konnte im Hefe-Zweihybridsystem eine Wechselwirkung der E2-Ligase mit TGA2.1 nachgewiesen werden, die die Reportergenaktivität des Hefestammes …
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Multiple Levels of Regulation of Cyclin-dependent Kinase Inhibitor 1A (p21/WAF-1) by the <i>DDX5 </i>Gene
The p68 protein, an archetypical member of the DEAD box family of RNA helicases, exhibits both ATPase and RNA helicase dependent/independent activities. To date the p68 protein has been implicated in most parts of RNA metabolism including ribosome biogenesis, transcription, RNA decay, miRNA …
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Biophysical characterization of the Escherichia coli RNA degradosome
… consist of the endoribonuclease RNase E, the DEAD-box helicase RhlB, the glycolytic enzyme enolase, and the exoribonuclease PNPase. The main scaffold component of the RNA degradosome, RNase E, contains an amphipathic alpha helix that recruits the degradosome to the cytoplasmic membrane. …
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