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Showing 1 to 20 of 44 for “"DAPI"”.
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Synthesis and Characterization of Novel Polyimide Gas Separation Membrane Material Systems
… 6)-amino-1-(4-aminophenyl)-1,3,3-trimethylindane (DAPI), 5,6-diamino-1-(4-aminophenyl)-1,3,3-trimethylindane (TAPI) and 6-hydroxy-1-(4-hydroxyphenyl)-1,3,3-trimethylindane (DHPI) were synthesized and characterized. DAPI, as well as other diamines, were then utilized in solution step …
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Apical Testis Structure and the Effects of Cadmium Treatment on Spermatogenesis in Drosophila
… we developed two assays: a nuclear staining (DAPI) assay to assess cadmium dosage effects on late spermiogenesis and a Live/Dead assay to assess mature sperm viability. The results of the DAPI assay and the Live/Dead assay both show a detrimental effect by CdCl <sub>2 </sub>on spermatogenesis …
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Auditory Hair Cell Regeneration and Gene Expression in Noise-Exposed Zebrafish (Danio rerio)
… recovery were determined using phalloidin and DAPI labeling to visualize hair cell bundles and nuclei, respectively. Cell proliferation was quantified through BrdU labeling. Immediately following noise exposure, zebrafish saccules exhibited significant hair cell bundle loss and reduced DAPI …
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Study on gold nanoparticles for biological applications
… a DNA dye, 4'-6-Diamidino-2-phenylindole (DAPI), and different types of gold nanoparticles in solution is demonstrated using FLIM. Biological imaging application based on energy transfer between gold particles and DAPI in cell culture is discussed as well in this chapter. A study on energy …
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Response of indigenous heterotrophic groundwater bacteria to low organic substrate availability
… were determined by 4'6-diamidino-2-phenylindole (DAPI) epifluorescent counts. The percentage of those bacteria which were metabolically active was determined by a modification of the 2-(p-iodophenyl)-3-(p- nitrophenyl)- 5-phenyl tetrazolium chloride (INT) reduction method. Advantages of this …
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The role of enteric bacteria in the Abalone, Haliotis Midae
… were enumerated using culturable and total (DAPI) counts. The numbers of culturable bacteria fell between 3.5x10⁵ and 2.3x10⁸ cfu/g wet weight tissue. The DAPI counts were between 1.6x10⁹ and 5.1x10⁹ cells per gram of tissue. The numbers of bacteria differed between the crop, stomach and …
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Discovery of new biomarkers of mild cognitive impairment and Alzheimer's disease risk in buccal cells using laser scanning cytometry
… antibodies (for detection of Aβ and Tau) and/or DAPI, Fast Green and Oil Red O dyes. In an initial study an LSC protocol was developed to identify and measure differences in buccal cell types and nuclear DNA content as well as a significant increase in micronuclei measured in AD (n=10) and Down’s …
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Analyse der Chromosomenverteilung in menschlichen Fibroblasten mittels 3D-Vielfarben Fluoreszenz in situ Hybridisierung
… was used for imaging of the 7 fluorochromes plus DAPI. Multicolor images from nuclei were taken as serial sections in z-direction. After deconvolution, a specifically developed Program goldFISH Saracoglu K. et al. 2001 was used to classify the images according to the labelling scheme. The …
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Maduración in vitro de ovocitos de perra provenientes de folículos poliovocíticos
… los ovocitos previa fijación y tinción nuclear DAPI mediante microscopía de epifluorescencia. El desarrollo meiótico se clasificó en vesícula germinativa (VG), reinicio meiótico (GVBD) y metafase I y II (MI-MII). Los resultados analizados por ANOVA, indicaron que, aunque los ovocitos de FPOs …
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Novel polyelectrolyte complexes for oral insulin delivery.
… Caco-2 cells using fluorescence microscopy with DAPI staining indicated that uptake of QPaa and QPaa-TBA complexes was mainly intracellular being localised within the perinuclear area of cells highlighted by DAPI. Hence, intracellular uptake of PECS by Caco-2 cells was enhanced by Paa …
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Development of New Regeneration Methods in Animal Models with Chronic Tympanic membrane Perforations Using Tissue Engineering Techniques
… cell markers), Ki67 (proliferation marker), and DAPI. Rat models for acute or chronic perforations in TMs were prepared. Chronic perforations were produced by a new method - ‘Choung’s COM model I’. The TMs were regularly obtained from rat models with acute or chronic perforations and …
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ABHD16A-proteiinia inhiboivien yhdisteiden toksisuuden tutkiminen hiiren kortikaalihermosoluilla
… käytimme immunofluoresoivia NeuN- ja DAPI-vasta-aineita. Niiden fluoresenssi kuvattiin automatisoidulla mikroskoopilla ja solujen määrän laskemisessa käytettiin CellProfileria. Aluksi valittujen immunofluoresoivien MAP2-vasta-aineiden avulla solumäärää ei onnistuttu ohjelman avulla …
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Development of Tools to Assess the Effects of Lunasin on Normal Development and Tumor Progression in Drosophila Melanogaster
… mounted in Vectashield® with the nuclear stain, DAPI. Our results demonstrate that EGFP-Lun, like native Lunasin, is localized to the nucleus. Eight transgenic lines were mapped to specific chromosomes and EGFP-Lun transgenic line GEJ1-L2 was balanced in preparation for use in tumor suppression …
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Phenotyping Double Transgenic Mouse Models of Alzheimer’s that Express Human APP and ApoE3 or ApoE4
… thioflavin-S, and 4’,6-diamidino-2-phenylindole (DAPI) to quantify diffuse Aβ, fibrillar Aβ, and cellular count, respectively. PDAPP:E4 mice had more total Aβ in the CA1 of the hippocampus and dorsal cortex. Also, a trend suggested PDAPP:E4 mice had a lower cellular density in the CA1 and dorsal …
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Regenerationspotenzial CD133+-hämatopoetischer Progenitorzellen der humanen Nabelschnur beim Nierendefekt im Mausmodell
… der humanen VYSIS-Y-Chromosomensonde (grün) und DAPI vorgenommen. Mit verschiedenen molekularbiologischen und mikroskopischen Verfahren konnte die Migration/Integration humaner Stammzellen in murine Nieren nachgewiesen werden. Einzelne Zellen männlichen Ursprungs wurden in den proximalen …
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Energetics and Structural Aspects of Cation-Coupled Drug Transport by NorM Multidrug Transporters
… stutzeri (NorM-PS) was shown to transport DAPI by utilising proton cycling exclusively. NorM-VC and NorM-PS share 42% identical amino-acid residues and yet their functions differ in terms of their ion coupling properties. These differences in functionality of two highly homologous proteins …
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Eukaryotic Microbes in the Deep Sea: Abundance, Diversity, and the Effect of Pressure
… and one by morphology when stained with DAPI) . In the epipelagic 100 m samples, only 20% of total eukaryotes fall into one of these groups. This difference is driven largely by the morphotype I call the "split nucleus", which does not decrease in absolute abundance with depth, instead …
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Cellular interactions with hydrogels
… function. Calcein AM/ethidium homodimer-1 and DAPI assays were carried out using 3T3 and EKl.BR cells. Poly(HEMA) was found to be the most cell adhesive hydrogel tested. The reactivity of monomers and the resulting sequence distribution were found to affect surface properties and this may …
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Identity and dynamics of the microbial community responsible for carbon monoxide oxidation in marine environments
… by microautoradiography in combination with DAPI and fluorescent-labeled oligonucleotide probes (Substrate Tracking AutoRadiography - Fluorescent In Situ Hybridization (STAR-FISH)). Marine alpha group organisms were a major component of total cell numbers (45.7%) at the time of sampling …
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Automatic GUI Based Counting Software for Immunostaining Analysis Using Matlab GUIDE
… counting. The coefficients of determination for DAPI and β–galactosidase positive cells are good, but moderate in case of Sclerostin. This automated GUI software method is a major advancement in terms of osteocyte counting from histological sections, and the procedure is explicitly compatible to …
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