Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"D-lactate"”.
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The relationship between glucose metabolism byproduct, D-lactate, and vascular endothelial cell dysfunction and possible role in diabetes
… The glucose metabolic by-product, D-lactate, is elevated in diabetics and it is unknown whether it contributes to endothelial cell apoptosis. We hypothesized that diabetic D-lactate levels induce apoptosis in human vascular endothelial cells (HUV-EC-C). HUV-EC-C were incubated with …
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Studies on pyridine nucleotide-dependent processes in Haemophilus influenzae
… nucleotide pyrophosphatase. A cytosolic lactate dehydrogenase, specific for D(-)-lactate was purified to electrophoretic homogeneity 2100-fold with a 14% yield. The purified enzyme was demonstrated to be a tetramer of M, = 135,000. It catalyzes essentially the reduction of pyruvate with …
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The role of lactate in CD4+ T cell differentiation and function
In nature, lactate can be found in two different enantiomeric forms, D- and L-lactate. The only difference between both molecules is the rotation angle of one asymmetric carbon atom. However, distinct biological activities and metabolic pathways originate and process one or the other enantiomeric …
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Metabolic alterations to sudden introduction of high carbohydrate diets in ruminating dairy bull calves
… and diarrhea. Ruminal pH was lower and L- and D-lactates greater in the rumen of 81% TDN treatments. Total ruminal VFA decreased as pH declined. Proportion of acetate increased while propionate decreased in 81% TDN treatments. Butyrate differed but was not dietary related. Whole blood L-lactate …
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Systems Metabolic Engineering of Microbial Cell Factories for the Synthesis of Value-added Chemicals
… Chapter 5), via overexpression of a novel D-lactate dehydrogenase: encoded by <italic>gldA101</italic>). The production of D-lactate was further improved by employing three strategies:: i) cofactor balancing,: ii) codon optimization, and: iii) process optimization. The engineered …
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Effect of Oxygen-Limiting Tidal Conditions on Muscle Metabolism and Structure in the Giant Acorn Barnacle, Balanus nubilus
… to determine citrate synthase (CS) activity, lactate dehydrogenase (LDH) activity, and D-lactate levels. We also prepared a subset of SA and TD muscles from the chronic barnacles for histological analysis of fiber diameter (Feret’s), cross-sectional area (CSA), mitochondrial distribution and …
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Trends in Equine Nutrition and the Effects of a Hindgut Buffer Product in Overconditioned Horses
… (DB). Horses receiving DB had decreased plasma L-lactate (P=0.05), and a tendency for increased fecal pH (P=0.08) and decreased fecal D-lactate (P=0.07). These studies demonstrate a need to improve horse owner education and the relationship between representatives and trained nutritionists to …
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Physiological response of grazing horses to seasonal fluctuations in pasture nonstructural carbohydrates
… for glucose (mg/dL), insulin (μIU/mL), and L-lactate (mg/dL). Each month, fecal grab samples were collected from the midrectum to measure pH and Dlactate (µM). Additionally, monthly glucose and insulin dynamics (% ∆) were assessed via a modified oral sugar test. Pasture nutrients including DE …
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Understanding the Genome-Wide Response of Streptomyces coelicolor to the Glycopeptide Antibiotic Teicoplanin
… of D-Ala-D-Ala on lipid II to D-Ala-D-Lactate (VanHAX), reducing glyopeptide affinity by 1000-fold. Our previous study demonstrated that altering the termini of lipid II by VanHAX action is insufficient for providing resistance to teicoplanin in S. coelicolor, which is instead mediated …
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Pre-, pro-, and synbiotic modulation of the intestinal microbiota stimulates enteroendocrine mediated intestinal adaptation in piglets with short-bowel syndrome
… abundances of ileal and colonic butyrate and lactate-producing bacterial populations and their modulation of intestinal nutrient transport 3) Determined the associations between changes in the intestinal microbiota, intestinal epithelium, and enteroendocrine system related to intestinal …
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The Role of Advanced Glycation End Products (AGEs) through the TRPA-1/NRF-2 Activation Pathway in the Neurodegenerative Diseases
… that are responsible for catalyzing MGO into D-lactate preventing the formation of AGEs. Therefore, preventing the formation of excessive AGE buildup is essential for limiting the progression of various debilitating age-related disorders. The focus of this project is to investigate the …
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Multi-omics investigation of interactions between persistent bacteria and Salmonella in the inflamed gut
… Current dogma surrounding Salmonella lactate utilization from the host is also confronted by our data, which implies potential cross feeding on microbially derived D-lactate by Salmonella during peak infection. These expression data are supported by random forest and logistic …
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Nucleotide Inhibition of Glyoxalase II
… catalyzes the irreversible breakdown of SLG to D-lactate and GSH. We have observed that ATP or GTP significantly inhibits the Glo-II activity of tissue homogenates from various sources. We have developed a rapid, one step chromatography procedure to purify Glo-II such that the purified enzyme …
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Identification and characterization of virulence associated factors of C. jejuni
… donors, e.g. succinate, malate, formate, D-lactate, hydrogen and NAD(P)H. The possession of a sulphite oxidation system is believed to be important for C. jejuni to survive in food which has been treated with sulphite for preservative reasons, as well as in humans since it has been shown …
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Characterization of two novel proteins containing the rhodanese homology domain: YgaP and YbbB of Escherichia coli
… with the cytoplasmic membrane marker D-lactate dehydrogenase, and was not present in the outer membrane fractions, indicating YgaP is a cytoplasmic membrane protein. A polyhistidine-tagged variant of YgaP was subsequently solubilized from the membrane by detergent extraction and purified …
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Circadian and Seasonal Variation in Pasture Nonstructural Carbohydrates and the Physiological Response of Grazing Horses
… were calculated. In the April study, plasma L-lactate and fecal pH, L-lactate, D-lactate and volatile fatty acids (VFAs) were also measured. Two approaches were used for the determination of carbohydrate profiles in pasture forage samples. For the first (LAB1), sugar was water soluble …
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Library Screening and Focused Multiomics of Antibacterial Action on Vancomycin-Resistant Enterococci faecium
… D-Alanine-D-Alanine is replaced by D-Alanine-D-Lactate (Lac), to which vancomycin cannot bind effectively. While the general features of this resistance mechanism are well known, the details of the choreography between vancomycin exposure, vanA gene induction, and changes in the normal and …
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Identification and preliminary characterization of the 2,5-diphenyloxazole biosynthetic pathway in streptomyces polyantibioticus SPRT
… ΔCIN mutant strains encoding the putative D-lactate dehydrogenase encoded by gene SPR_60250, the PA-CoA ligase (paaK) encoded by gene SPR_46390 and the cinnamate CoA ligase encoded by gene SPR_60150, respectively. Based on the genome annotation analysis and gene disruption studies, a model …