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Showing 1 to 20 of 30 for “"D-amino acid"”.
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Characterization of D-amino acid-containing neuropeptides from metazoa
The predominance of left-handedness in amino acids and proteins in living organisms is well established. The presence of D-amino acids in the prokaryotes (e.g., bacterial cell wall and lantibiotics) has been considered exceptions to this rule. In the animal kingdom, proteins are thought to be more …
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The D-Amino Acid Content of Bacterial Spores and Vegetative Cells
Made available in DSpace on 2014-12-05T21:45:22Z (GMT). No. of bitstreams: 1 0006958.pdf: 1926158 bytes, checksum: b2b399651c0acd3848c68baec871818b (MD5) Previous issue date: 1953
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Analysis of D-amino acid-containing neuropeptides in mollusks and rodents
… are translated by ribosomes using exclusively L-amino acids. However, D-amino acids are present in animal peptides, first evidenced by dermorphin, an analgesic skin peptide in Phyllomedusa sauvagei. Dermorphin’s existence as a D-amino acid-containing peptide (DAACP) proved not to be a …
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Analysis, fractionation and exploratory recombinant expression of novel eukaryotic l/D peptide isomerases and free D-amino acid racemases
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-09-01 without embargo terms
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Analysis of endogenous D-amino acids in the central nervous system and endocrine system via capillary electrophoresis with biochemical sample treatment
… Due to their unique chirality, endogenous D-amino acids are considered to play different roles from their corresponding L-isomers. In the central nervous system, a few D-amino acids have been found and they have increasingly gained research interest because their localization suggests …
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Novel Approaches for the Measurement of Unusual Post-Translational Modifications in Neuropeptides
Other PTMs, such as the transformation of L-amino acid residues to the D-configuration, do not result in a measurable mass change. Capillary electrophoresis combined with mass spectrometry is used to identify cells containing a D-amino acid-containing peptide found in Aplysia. These cells are then …
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Analysis of enzymatic L/D-peptide isomerization in animals
… in animals is stereospecific for all L-form amino acids, a unique and bioactive group of D-amino acid-containing peptides (DAACPs) have been found to exist in a broad array of animals. DAACPs result from a post-translational modification (PTM) in which an L-amino acid residue is enzymatically …
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Synthetic Peptide Ligand Mimetics and Tumor Cell Motility
… a combinatorial screening approach, six novel D-amino acid containing peptides were identified and analyzed for their ability to adhere to human prostate tumor cells, support tumor cell adhesion and inhibit tumor cell adhesion to ECM proteins. Two peptides, RZ-3 (kmviywkag) and HYD1 (kikmviswkg) …
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Delivery of biomolecules into mammalian cells using anthrax toxin
… proteins, polypeptides containing non-canonical amino acids or small molecule drugs, and cyclic peptides. Our translocation analyses have led to guidelines for translocation as well as insight into design parameters for the efficient delivery of new cargos. The PA/LFN delivery platform has also …
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Neurotransmitter and Neuromodulator Analysis Using Capillary Electrophoresis With Laser -Induced Fluorescence Detection
… L-Asp. A method for single cell analysis of D-amino acid containing peptides in Aplysia abdominal neurons has been developed and the D-form dominates in one diastereomer pair. Lastly, in preliminary studies, the release of L-Glu versus acetylcholine from specific regions of the brain of freely …
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In vitro metabolism of uniformly labeled glucose-C14 by bovine rumen microorganisms
… matter “precipitate“, (c) ether extract, (d) “amino acid“, (e) “sugar“, (f) CO₂, and (g) CH₄. Similar fractionation of an unfermented control sample was made. A portion of the fermentation ether extract was subjected to column chromatography to resolve (a) C₁, (b) C₂, (c) C₃, (d) C₄, and (e) C₅ …
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Discovery of high quality molecular leads and screening collections for CNS drug targets
… allowed the de novo design of a novel class of D-amino acid oxidase (DAAO) inhibitors. This work gave rise to three novel series of DAAO inhibitors, all of which were capable of addressing some or all of the issues that had limited previous attempts to inhibit this high profile CNS target. …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
… encoded hydrogen peroxide generator, D-amino acid oxidase (DAAO), was used to understand the hours-long intracellular hydrogen peroxide (H₂O₂) generation. This study elucidated that the primary respondent of cytosolic H₂O₂ is peroxiredoxin 1 and the H₂O₂ induced apoptosis initiates …
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Development of Novel Small-Molecule Therapeutics for Multiple Sclerosis
… of MS. Chapter 5 explores the utility of D-amino acid analogs as PAD inhibitors with the specific aim of improving the pharmacokinetics and metabolic stability of these molecules. Chapter 6 concludes the thesis by discussing knowledge gained throughout and provides a perspective on future …
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Characterization of the Broad-Spectrum Racemase, BsrF, in Vibrio fischeri
L-amino acids are used by all living organisms as the building blocks of proteins and are well characterized, while D-amino acid enantiomers are less common, and their roles are still being elucidated. Broad-spectrum racemases (Bsr), which have been found in several Bacteria and one Archaeon, can …
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Identification, characterisation and design of bioactive peptides from Chinese frogs
… and stability, DMPC-1OB was designed using D-amino acid residues. Through antimicrobial assay, cytotoxicity assay and in vitro and in vivo stability assays, it was found that DMPC-1OB had strong and broad-spectrum antimicrobial activity, low cytotoxicity and high stability in vitro and in …
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Structure–Activity Analysis of Peptide-Mediated Quorum Sensing in Streptococcus mitis
… responsible for activity, and the second, a D-amino acid scan to evaluate the effect of sidechain spatial orientation. Then, following the construction of a luminescence-based S. mitis NCTC 8033 QS reporter strain, we conducted reporter gene bioassays to gain insights into the …
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Investigation and application of heterochiral proteins enabled by flow-based peptide synthesis
Natural proteins are comprised primarily of (L)-amino acids. (D)-amino acids are rare in protein structures. Solid-phase peptide synthesis (SPPS) and native chemical ligation enable the total chemical synthesis of proteins. Using these techniques, it is possible to design and study polypeptides …
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Enantioseparation using a counter-current bioreactor
… using as a model reaction the isolation of L-amino butyric acid (L-ABA) from a DL-ABA racemate and the enantioselectivity of D-amino acid oxidase (DAAO). Bioconversion of D-ABA to ketobutyric acid (KBA) by DAAO, immobilised by selective partitioning in the stationary phase of the CCC …
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Biochemical Characterization of CXCR4 Interactions With HIV -1, Natural Ligands and De Novo Designed Inhibitors
… SMM-chemokines, particularly those unnatural D-amino acid-containing analogs, share many CXCR4 binding sites with HIV-1 gp120 and yet differ from SDF-1alpha.
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