Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Cytosolic Delivery"”.
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Amphiphilic gold nanoparticle-based cytosolic drug delivery platform for cancer and infections
… cells is immunosuppression. Deregulation of cytosolic kinases negatively alters immune cell signaling, proliferation, differentiation, and migration. Small molecule kinase inhibitors offer unique opportunities to enhance anti-tumor immunity by inhibition of immunosuppressive kinases. However, …
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Escape to the interior: Characterization of the cytosolic delivery of C-terminal cargo subdomains by the N-terminus of pasteurella multocida toxin and subsequent toxin-mediated downregulation of Gaq-PLCb1 signaling through depletion of Gaq from the plasma membrane
… to answering these two major questions regarding delivery of C-terminal cargo by PMT-N into the host cytosol and its subsequent downregulation of Gaq-protein signaling in intoxicated cells. To address the first question, we hypothesized that PMT-N delivers the C- terminal cargo into the cytosol of …
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Development of a Real-Time Cell Imaging Assay to Characterize the Delivery of a Cell-Penetrating Peptide Adaptor and its Associated Cargo
… problem is the main rate-limiting step for cytosolic delivery and is a result of endocytosis. To combat this issue, a new CPP, TAT-CaM, that can reach the cytosol has been developed. Conventional methods involve observing these peptides in a static manner, which has hindered a complete …
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Fluorogenic probes for live-cell imaging of biomolecules
… that exhibits unusual properties: efficient cytosolic and nuclear uptake. This dextran permeates various human cell types without the use of transfection agents, electroporation, or membrane perturbation. Cellular uptake occurs primarily through active transport via receptor-mediated …
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Comparative analysis of cytotoxic necrotizing factor (CNF) toxins: Compatibility of cargo with delivery vehicle and identification of amino acid residues that modulate pH-dependent cytosolic cargo delivery
… are increasingly being exploited for targeted delivery in biomedical and research applications due to their potency and cell specificity. This thesis explores the suitability of the cytotoxic necrotizing factor (CNF) family as a model system for the development of Bacterial Toxin-Inspired Drug …
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A technical and economic evaluation of novel pH-responsive core-shell nanoparticles : delivering innovation from laboratory to market
… for the treatment of disease require the delivery of drugs into the cytosolic or nuclear compartments of cells. Members of the Irvine laboratory have developed a novel pH-responsive core-shell nanoparticle system that can achieve efficient and non-cytotoxic drug delivery into the cytosol. …
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Cell squeezing : a vector-free microfluidic platform for intracellular delivery of macromolecules
Intracellular delivery of material is a long-standing challenge for both therapeutic and research applications. Existing technologies rely on a variety of mechanisms to facilitate delivery. Vector-based methods, such as polymeric nanoparticles and liposomes, form complexes with the target material …
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Synthesis of pH-responsive core-shell nanoparticles of different sizes and with different shell compositions
… administered vaccines and facilitate the cytosolic delivery of a wide variety of therapeutic macromolecules. One of the goals of this study was to reduce the size of these core-shell nanoparticles to improve their permeation into the skin. Separate nanoparticle syntheses using reduced …
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pH-sensitive core-shell nanoparticles for intracellular drug delivery
… is a major challenge for intracellular drug delivery. To address this issue, we designed a pH-sensitive core-shell nanoparticle to segregate the functions of the particle into an endosome-disrupting pH-responsive core that would absorb protons at endolysosomal pH, and a shell whose …
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Development of high-throughput nanoparticle screening technologies to facilitate the delivery of genetic therapies to non-liver cell types
… to the diseased cell type. One promising delivery method is through the use of Lipid Nanoparticles (LNPs), due to their ease of manufacture and favorable toxicity profiles. Yet, LNPs have only seen clinical success as delivery methods to liver cells. In order to treat genetic diseases …
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The characterisation of a recombinant cytosolic shuttle based on ricin toxin
Cytosolic access is rate limiting for potential therapeutics such as antisense agents. In nature, several entities deliver macromolecules to the cytosol, one example being ricin toxin (RT). In this thesis, a cytosolic drug delivery system based upon attenuated RT was characterised. The …
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Degradable polymeric nano-films and particles as delivery platforms for vaccines and immunotherapeutics
… by acting as platforms that facilitate the delivery of molecules to appropriate tissue and cellular locations to achieve therapeutic outcomes. To this end, we have designed and characterized nano-films and particles employing a hydrolytically degradable polymer for the delivery of vaccine …