Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 391 for “"Cytosol"”.
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The ubiquitin coat of cytosol-invading bacteria
Cytosol-invading Salmonella enterica serovar Typhimurium are labelled with a ubiquitin coat, which serves the dual purpose of targeting bacteria to autophagy-mediated degradation and locally inducing pro-inflammatory signalling. Importantly, the chain types and ubiquitylated substrates that …
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The Mechanisms of Tau Entry to the Cytosol
… protein tau forms filamentous assemblies in the cytosol of neurons in several neurodegenerative diseases. In Alzheimer’s disease, tau pathology arises throughout the human brain in a spatio-temporal manner and closely correlates with disease severity. Assemblies of tau have been proposed to …
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Dynamics of E. coli genome and cytosol under antibiotics
… of fluorescently-tagged chromosomal loci and cytosolic aggregates, which report for the state of locus ”compaction” and the levels of macromolecular crowding of the cytosol, respectively. We improve the precision of those measurements developing a novel data treatment procedure and discover …
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A 450K arsenite-binding protein of the rabbit liver cytosol
… to animals is primarily associated with liver cytosolic macromolecules. The specifics of this binding and its role in arsenic toxicity have never been fully examined. This dissertation concentrated on determining the species of inorganic arsenic which binds to liver cytosolic proteins, the …
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Androgen aromatization and cytosol estradiol-receptors in the mediation of masculine sexual behavior in Japanese quail
… in vivo aromatization of ³H-testosterone and (2) cytosolic estradiol-binding by in vitro saturation analyses. After in vivo incubation with ³H-testosterone, all radioactivity recovered in brain tissues was in the form of testosterone, dihydrotestosterone, or estradiol. Neither the total ³H nor …
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Identification of host factors required for cytosol entry by Shigella flexneri: Results of a genome-wide CRISPR/Cas9 screen
… the vacuole membrane to gain entry to the host cytosol. As a professional cytosol-dwelling pathogen, *Shigella* then overcomes cell-autonomous host defences to establish its replicative niche, before spreading into adjacent enterocytes. Although cytosol entry is a key step in its cellular …
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Studies on the mode of action of dermonecrotic toxins: Effects on adipogenic signaling pathways and delivery of c-terminal cargo into the cytosol
… most of the cargo (GFP, PMT-C) intact into the cytosol, and the N-terminal portion of the toxin gets further processed into multiple, smaller fragments, which remain associated with vesicles. Taken together, we outline a mechanism whereby after uptake and trafficking to endosomes, PMT is …
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Influence of Cellular Localization on Activity of Hydroxysteroid Dehydrogenases
… hormones. HSDs use nicotinamide cofactors in the cytosol and endoplasmic reticulum (ER) lumen to either reduce or oxidize their steroid substrates. Our lab has extensively studied the 17beta-HSDs types 1, 2 and 3 of the short-chain oxidoreductase family, particularly human 17beta-HSD1, which …
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Innate Immunity to Listeria monocytogenes Infection: Regulation of Dendritic Cell and Neutrophil Responses by MyD88 and the Type I IFN Receptor
… gains access to the host cell cytosol via the pore forming toxin listeriolysin O (LLO) and infects a variety of cell types including dendritic cells (DC). Our laboratory and others have shown that optimal DC maturation occurs when <italic>Listeria</italic> gain access to the …
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MOLECULAR REGULATION OF ATP-CITRATE LYASE
… for metabolic disorders and cancer. In the cytosol, acetyl-CoA is used for lipid synthesis and is also the essential substrate for protein acetylation. In the nucleus, acetyl-CoA is used for histone acetylation, which is particularly sensitive to glucose availability and ACLY deficiency. …
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Quo vadis: quantitative evaluation of intracellular kinetics of siRNA delivered by poly-and-lipoplexes
… molecules to their primary sites of activity in cytosol. This, however, only solved the problem in part, since such vectors interact with oligonucleotides to form particles that, as commonly believed, require endocytosis to enter the cells. Endocytosis is a process by which cells communicate with …
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IN-VITRO METABOLISM AND PROTEIN BINDING OF 5-HMF, A POTENTIAL ANTISICKLING AGENT
… the metabolic pathways of 5-HMF in human hepatic cytosol, to assess inter-species differences in its hepatic metabolism, and to predict in-vivo PK properties. Moreover, metabolism of 5-HMF in human RBCs was investigated. Finally, in-vitro studies were done to characterize 5-HMF binding kinetics …
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Sphingomyelin as a danger signal in cell-autonomous immunity
… of cell-autonomous immunity to defend their cytosol against bacterial invasion. One such mechanism involves indirect detection of the pathogen through recognition of pathogen-induced disturbances causing the appearance of specific host molecules in an abnormal location. For example, glycans, …
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Characterisation of the sulfotransferases catalysing the sulfation of xenobiotics and steroids in bovine liver
… like SULTs. These include tissue microsomes, cytosol, recombinant enzymes and isolated cells such as the hepatocytes.Recombinant SULTs are important tools that can be used for the study of isoform specific drug biotransformation, drug-drug interactions and the effect of genetic polymorphisms …
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Approaches to study Zn(II) deficiency and transport in biology
… the transition metal ion concentration of the cytosol and 10 ZnTs that decrease the transition metal ion concentration of the cytosol. Zn(II) homeostasis can be challenged either by excessive or inadequate nutritional Zn(II) or by interference of other metal ions with Zn(II) uptake …
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pH-sensitive core-shell nanoparticles for intracellular drug delivery
… siRNA, can only exert their function in the cell cytosol or nucleus. However, most of them are cell membrane impermeable molecules that can only be taken up by cells via endocytosis or phagocytosis. Such drug molecules are thus confined in endolysosomes, where reduced pH and degradative enzymes …
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Innate Immune Sensing and Signaling of Cytosolic DNA
… the nucleus and mitochondria. DNA exposed in the cytosol is a danger signal that warns the host of invading microbial pathogens and triggers innate immune responses including the production of type-I interferons (IFNs). Endogenous DNA that is inappropriately cleared can also accumulate in cytosol …
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Novel detection and evasion mechanisms pertinent to immunity against Salmonella Typhimurium
Cells defend their cytosol against pathogen invasion using cell-autonomous immunity. When pathogens enter the cytosol they can damage host endomembranes, causing the mislocalisation of host molecules not normally found in the cytosol that are sensed as Danger Associated Molecular Patterns (DAMPs). …
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The role of a pore-forming protein, Mpeg1, in cytosolic import
… for cross-presentation have been described, cytosolic and vacuolar. In the cytosolic pathway, antigens need to be imported from the endo/lysosomal compartment (to which they localise after their uptake) to the cytosol for further processing by the proteasome, which then generates peptides …
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Molecular Mechanisms of Inward and Outward Budding From Multivesicular Endosomes
… the MVB membrane. Both budding reactions are cytosol-dependent and supported by <em>Saccharomyces cerevisiae</em> (yeast) or <em>Drosophila melanogaster (fly)</em> cytosol, providing a system amenable to genetic manipulation. We observed that inward and outward budding are mechanistically …
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