Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Cytosine Base Editing"”.
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Base editing of Galactose-1-Phosphate Uridylyl Transferase as a novel gene therapy approach to treat Q188R mutation in a cellular model of Classic Galactosemia
… galactitol. The Q188R mutation is caused by a base change from adenine to guanine in base pair 563. This mutation causes an amino acid change in position 188 from glutamine (Gln) to arginine (Arg) and is responsible for 60-70% of CG cases in homozygous state. Consequently, affected infants …
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Determining the Roles of Q-SNAREs in Mast Cell Tumor Necrosis Factor Exocytosis
… Stx2, -4, -6, and –11 KO RBL-2H3 cells using Cytosine Base Editing, and successfully generated heterozygous KO cell lines of all four genes. The results of these investigations have revealed that Stx2 may be dispensable for mast cell exocytosis in general potentially significant roles for Stx3 …
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CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
… numerous effectors, which are classified based on their function. m6A is deposited co-transcriptionally by “writers”, including methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) that form a heterodimer regulated by the METTL-associated complex (MACOM). Its removal …
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Genetic Engineering as a Tool to Investigate Mitochondrial Gene Expression
… mitochondria, in particular the development of base editing, it is now possible to investigate these processes in more detail than ever before. Using these reverse genetics approaches, this work aims to utilise this new toolkit to probe different aspects of mitochondrial gene expression and …