Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Cytolytic activity"”.
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Suppressive Effects of Transforming Factor-β and Interleukin-10 on the Cytolytic Activity of Murine Macrophages and Reversal by Cytokines
… peptone-activated macrophages was compared. The cytolytic activity for tumor cells of these effector cells was measured by percent lysis of <sup>3</sup>H-thymidine labeled Herpes simplex virus type 2-transformed tumor cells (H238). After 18-24 hours of incubation with TGF-β or IL-10, the …
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Integrative genomic approaches to dissecting host-tumor and host-pathogen immune processes
… solid tissue tumor biopsies, we quantified the cytolytic activity of the local immune infiltrate and identified associated properties across 18 tumor types. The number of predicted MHC Class I-associated neoantigens was correlated with cytolytic activity and was lower than expected in colorectal …
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In vitro cytotoxic activity of equine lymphocytes on equine herpesvirus-1 infected allogenic fibroblasts
… to analyze the <i>in vitro</i> cytotoxic activity of lymphocytes from adult horses against equine herpes virus-1 (EHV-1) infected allogenic equine dermal fibroblasts (EDF); 2) evaluate the ability of a 72 hour in vitro incubation with interleukin-2 (I L-2) to enhance the lymphocytic …
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Human macrophage model for selective evaluation of CD8⁺ and γδ⁺ cytotoxic T cell function in tuberculosis
… to autologous macrophage targets. This cytolytic activity was restricted to live organisms since only U937 cells infected with virulent H37Rv M. tuberculosis and BCG but not those pulsed with soluble PPD were lysed by the HLA class I-matched effector cells. On the other hand, M. …
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Role of Stat3 In Human Nk Cell Functions
… ligands expressed on stressed cells induce cytolytic activity of NK cells. Stimulation of NK cell proliferation and priming of NK cytolytic capability are accomplished by cytokines, which mediate their signals mainly through JAK-STAT signaling pathway. Previously, we found that K562 cells …
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Mechanistic and synthetic studies on the prochlorosin and cytolysin families of lanthipeptides
… (RiPPs) that commonly feature antibacterial activity and contain the characteristic thioether residues lanthionine (Lan) and methyllanthionine (MeLan). Installation of thioether crosslinks in lanthipeptide biosynthesis is carried out by designated synthetases and involves dehydration of …
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A Single Cell Approach to TCR Signal Strength
… killing their targets through the release of cytolytic granules. T cell receptor (TCR) recognition of foreign peptides presented by Class I MHC molecules stimulates naïve CD8+ T cell differentiation to effector cells and triggers the cytolytic activity of effector CTLs. Signal transduction …
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Discovery and Characterization of an Antibiotic from the Soil Bacterium <em>Bacillus</em> sp.
… a species of <em>Bacillus</em> was found to have activity against <em>Micrococcus luteus</em> and <em>Rhodococcus erythropolis</em>. BTHX2 has a 16S rDNA sequence 97% homologous to <em>Bacillus licheniformis</em>, and may be a new strain of <em>B. licheniformis</em>. The inhibitory substance …
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Role of CD44, Fas Ligand, and Perforin in the Cytotoxicity Mediated by Natural Killer Cells
… molecule, can participate in signaling cytotoxic activity of cytotoxic T lymphocytes (CTLs). In the current study we tested the hypothesis that activation of natural killer (NK) or lymphokine activated killer (LAK) cells induces the expression of FasL, perforin, and CD44 which together contribute …
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Immunotherapy in multiple myeloma
… T cells, which exhibit considerable anti-MM cytolytic activity. However, the DC from MM patients still display abnormal chemokine receptor expression, which may inhibit their capability to migrate to lymph nodes in-vivo in order to generate these cytotoxic T cell responses. These observations …
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Synergistic anti-tumor immune response to combination immunotherapy consisting of anti-tumor antibodies, extended half-life Interleukin-2, and other immunomodulatory agents
… NK cells, and CD8+ T-cells that appear to direct cytolytic activity against tumor cells. This combination therapy also induces an intratumoral "cytokine storm," potentially re-polarizing the tumor microenvironment into one that is immunologically anti-tumor. We also identify cross-talk between NK …
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Improving Nk Cell Therapy For Osteosarcoma
… to TGFβ- Resistant NK (ReNK). ReNK retain high cytolytic activity and produce remarkably more IL-6, IFNγ, and TNFα plus and minus TGFβ treatment compared to Standard NK cells. We characterized the TGFβ pathway in ReNK and found a near complete loss in SMAD3 protein and decreased TGFBR3 …
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The Multifaceted Contribution of Natural Killer Cells During Herpes Simplex Type-1 Viral Infection.
… cytokines induce NK cell activation, cytolytic activity, and cytokine production, contributing to innate immune response and ultimately influencing the adaptive immune response. The objective of this study was to elucidate the multiple roles of NK cells during the numerous steps in …
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Single-cell technologies for monitoring interactions between immune cells
… This platform enables integrated analysis of cytolytic activity, secretory activity, receptor expression, and dynamic parameters of interactions between thousands of individual NK cells and target cells. Using this platform, we show that NK cells operate independently when lysing a single …
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Augmenting Natural Killer cell antibody binding to enhance ovarian and prostate tumor cell killing via ADCC
… types, but strategies that enhance NK cell cytolytic activity have not been thoroughly investigated for the treatment of ovarian cancer or mCRPC. My research focused on developing approaches to boost CD16A function to improve the efficacy of mAbs. To enhance NK cell binding to …
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The roles of the chemokines CXCL12 and CXCL16 in breast cancer.
… and CXCL16 and antagonists of CXCL12 and CXCL16 activity, CXCL12[subscript](P2G) and CXCL16[subscript](9-220) respectively, were overexpressed in the 4T1.2 mouse model of breast carcinoma. Overexpression of wild-type CXCL12 potently inhibited both primary tumour growth and metastasis in this …
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Investigating the spatiotemporal dynamics of mononuclear phagocytes in solid tumours.
… organs, where they prime anti-tumour T cell activity. In this work, we aimed to resolve the spatiotemporal dynamics of MNPs following tumour infiltration and investigate how immune checkpoint blockade (ICB) treatment influences this. We utilised a photo-convertible mouse model, combined with …
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The clonal architecture and tumour microenvironment of breast cancers are shaped by neoadjuvant chemotherapy
… during treatment. The immune TME composition and activity mirrored response to treatment, with cytolytic activity and innate and adaptive immune infiltrates linearly correlating with the degree of residual disease remaining after chemotherapy. Finally, the circulating tumour DNA (ctDNA) genomic …
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Modeling of NK Cells in Pediatric Patients With Unusually Severe or Recurrent HSV Using High-Dimensional Flow Cytometry
… between severe infection and defects in NK cytolytic function. <em>PLCG2</em> haploinsufficient variants found in 2 HSV patients causing PLCγ2 hypophosphorylation, and loss of cytolytic function in NK cells is a novel finding recently published by Alinger et al from our lab in 2023. Prior to …
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The isolation, structure, and membrane interactions of biologically active peptides.
… eupus mongolicus. These peptides exhibit cytolytic effects against a number of eukaryotic and prokaryotic cells at micromolar concentrations, and their peptide sequences share similarities with other antimicrobial peptides from scorpions, arthropods and amphibian species. The secondary …