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Showing 1 to 20 of 103 for “"Cytochromes."”.
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Ligand-Protein Interactions in Cytochromes
Cytochromes are found in all living beings and play an essential role in life processes. The two proteins studied in this thesis, cytochrome P450 carries out enzymatic reactions, metabolizes drugs, and synthesizes steroids while cytochrome c553 is a protein involved in electron transfer and …
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Investigating Cytochromes in Photosynthetic Electron Transport in Cyanobacteria
Inhibitor binding sites in the cytochrome bf complex of Synechococcus 7002 was modified by site-directed mutagenesis. The aim of this study was to identify the amino acid residues in cytochrome b6 that participate in plastoquinol and inhibitor binding at the plastoquinol oxidation site of the …
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The fluroxene mediated degradation of cytochromes P-450
The degradation of cytochromes P-450 by fluroxene (2,2,2-trifluoroethyl vinyl ether) has been investigated. Fluroxene is shown to specifically degrade cytochromes P-450 in vivo and in vitro without affecting the levels of the other microsomal enzymes, cytochrome ~S a.nd NADPH-cytochrome~ reductase. …
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Ligand Binding and Structural Dynamics in C-Type Cytochromes
The first molecular dynamic simulations of the interaction of cyt c and yeast bc1 complex are described. Contrary to results from crystallographic studies, the simulations reveal multiple dynamic hydrogen bonds and salt bridges in the cyt c-c 1 interface. A novel structural change in cyt c is also …
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Mechanistic Studies of Two Steroidogenic Cytochromes P450, CYP17A1 and CYP21A2
Human CYP17A1 (P450c17 or 17alpha-hydroxylase/17,20-lyase) is a member of the P450 superfamily of proteins. This enzyme is responsible for hydroxylating the 17-position of pregnenolone or progesterone and cleaving the 17,20-carbon,carbon-bond of its hydroxylated products, which leads to the …
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Investigating the evolution of cytochromes P450 involved in GA biosynthesis
… kaurene oxidases (KO), which are multifunctional cytochromes P450 that catalyze oxidation at the C4α methyl, converting ent-kaurene to ent -kaurenoic acid, an early step in gibberellin phytohormone biosynthesis. Arabidopsis produces no labdane-related diterpenoids other than gibberellins, whereas …
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Physical Studies of the C-Type Cytochromes From Rhodospirillum Rubrum
Made available in DSpace on 2014-12-14T04:27:44Z (GMT). No. of bitstreams: 1 7820926.pdf: 5269254 bytes, checksum: ec5798c77b6c8db1d435d08054328264 (MD5) Previous issue date: 1978
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Mechanistic investigation of human cytochromes P450 involved in hormone biosynthesis
CYP17A1 and CYP19A1 are the key cytochromes P450 involved in steroidogenesis. Mutations causing hypo- or hyper-activation of both these enzymes results in diseased states, including cancers. In addition to carrying out mono-oxygenation reactions, both CYP17A1 and CYP19A1 also catalyze carbon-carbon …
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Structural and biochemical characterization of diheme <i>c</i>-type cytochromes
Cytochrome sind eine weit verbreitete Proteinfamilie die Häm als prosthetische Gruppe beinhalten und in Vorgängen der Elektronenleitung oder Redoxreaktionen involviert sind. Das Hämmolekül besteht aus einem aromatischen Porphyrinring und einem zentral gebundenem Eisenatom. Cytochrome des c-Typs …
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Immunological Characterization of the Cytochromes of Escherichia Coli (Hybridonas, Antibodies, Membranes)
(low aeration)(TABLE ENDS)
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Characterization of cytochromes and plastocyanin in the cyanobacterium Synechocystis sp. PCC 6803
The main objective of this project is to study the components involved in electron transport between the cytochrome bf complex and photosystem I in the unicellular cyanobacterium Synechocystis 6803. Two soluble proteins, the heme-protein cytochrome c553 and the copper protein plastocyanin, were …
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CHARACTERIZATION OF LOW MOLECULAR WEIGHT C-TYPE CYTOCHROMES IN CYANOBACTERIA AND PLANTS
Cytochrome c6 (Cyt c6) and Plastocyanin (Pc) are two well characterised redox carriers in the thylakoid lumen of cyanobacteria and algae. In cyanobacteria Cyt c6 or Pc shuttles electrons from the Cyt b6f complex to Photosystem I (PSI) or the terminal oxidase, and in plants Pc transfers electrons …
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Studies on cytochromes and electron transport in Methanosarcina thermophila strain TM-1
… in order to facilitate biochemical studies. Cytochromes occurring in Methanosarcina thermophila were characterized spectrophotometrically using chemical and physiological reactants. This analysis revealed two heme centers, one of which was only reduced by Na₂S₂O₄ or carbon monoxide. Partially …
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The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
"In order to characterize this secondary-binding interaction, a novel surface plasmon resonance technique termed ""analyte gradient-surface plasmon resonance"" (AG-SPR) has been developed and is described here. AG-SPR can be used to rapidly characterize macro-molecular interactions without …
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Engineering Enhanced Cytochromes P450: Understanding Thermostability and Mechanism Through Rational Design and Directed Evolution
Although random and rational approaches for protein engineering are often used as separate approaches, this work has demonstrated the benefits of combining these approaches. Through the use of both methods a better understanding of thermostability, catalytic mechanism and substrate specificity was …
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Identification and Characterization of the Cyd Gene Locus of Escherichia Coli (Respiration, Membranes, Cloning, Energetics, Cytochromes)
… complex that is composed of two additional cytochromes, b(,595) and b(,558). The first mutants affecting the cytochrome d complex (Cyd) have been isolated using two newly developed genetic procedures. These mutations are located in the gene locus, cyd, that mapped to minute 16.5 on the …
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Microarray and molecular genetic analysis of aberrant splicing in human drug metabolizing cytochromes P450 CYP2D6 and CYP2B6
This study was devoted to the detection of alternative splicing within the Cytochrome P450 enzymes 2D6 and 2B6, mapping of the most common splice variants and to draw connections to certain single nucleotide polymorphisms (SNPs) and alleles. For both enzymes a splicing sensitive microarray was …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
To expand our understanding of helical propensity and residual structure in the denatured state, two approaches have been taken. In the first project, we have engineered serine in place of alanine near the center of the third helix (positions 83 and 87) in cytochrome c’ (Cytc’) and have measured …
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