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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Cytochrome B5"”.
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The Effect of Phospholipid Polar Head Group Supplementation on The Microsomal Cytochrome B5 System
The maintenance of a fluid phospholipid bilayer is important for a number of membrane associated functions. The fluidity of the bilayer is determined by the lipid composition. The lipid composition can be described in terms of the phospholipid polar head group composition, the fatty acid …
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Engineering the interface of cytochrome b5 and myoglobin for in vitro and in vivo applications
… its physiological electron transfer partner, cytochrome b5, fast electron transfer and O2 consumption was achieved, making our protein model system to have a catalytic rate similar to that of native HCOs. A key to the above success was engineering the electrostatic interactions between CuBMb …
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Effects of Immobilization Matrices and Protein Orientation on Biomolecular Recognition at Solid-Liquid Interfaces
… proteins and soluble ligands. Oriented cytochrome b5 monolayers were constructed on supported lipid membranes using a site-selective immobilization method. The orientation of the immobilized cytochrome b5 was well controlled as verified with linear dichroism measurements. The …
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Vliv cytochromu b5 na enzymovou kinetiku hydroxylace Sudanu I lidským cytochromem P450 1A1
Cytochromes P450 are the major xenobiotics converting enzymes. They are classified as mixed function monooxygenases (MFO). Isoform 1A1 is a extrahepatic form found mainly in the lung and other tissues. It is strongly induced by polycyclic aromatic hydrocarbons and their derivatives via the Ah …
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Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops
Three cytochrome P-450 forms were highly purified (8-12 nmol/mg) from the hepatic microsomes of the untreated coastal marine fish Stenotomus chrysops (scup) by detergent solubilization and column chromatography. Scup cytochromes P-450A, P-450B and P-450E (labeled in order of elution from the first …
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Effects of Immobilization Matrices and Protein Orientation on Biomolecular Recognition at Solid-Liquid Interfaces
… physical techniques. The recognition between cytochrome b5 and cytochrome c was chosen as the model system to both identify and quantify the forces that originated from the immobilization substrates and the protein immobilization orientations. These measured forces were correlated with the …
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Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems
… each having different characteristics; cytochrome c association to cytochrome b5, unfolding of staphylococcal nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site.
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Developing novel systems for expression of intracellular membrane-bound human cytochrome P450 enzymes in baker’s yeast
Human cytochrome P450 (CYP) enzymes belong to a family of monooxygenase enzymes that are responsible for the metabolism, in a stereo-specific and stereo-selective manner, of diverse chemicals. These chemicals are often referred to as xenobiotics which are usually harmful and toxic to the human …
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
… proteins with the soluble heme-binding domain of cytochrome B5 (CytB5) and an N-terminal hexahistidine (6xHis) tag for purification. The CytB5 domain was linked to the native N-termini of active rhCatG and rHNE by the EPLcleavable substrate sequence DDDDK~I, where ~ is the sessile bond. These …
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Catalytic promiscuity of two plant P450 enzymes: CYP725A4 from Taxus cuspidata and CYP71B102 from Isatis Tinctoria
Plants are abundant in cytochrome P450s constituting about 1% of their protein coding genes. Some of these P450s catalyze oxidation reactions in metabolic pathways that lead to valuable compounds, like the anticancer drug paclitaxel, the blue pigment indigo and the promising antileukemic agent …
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The Golgi Complex of the Hepatocyte: Isolation and Partial Enzomological Characterization
… have no detectable glucose-6-phosphatase, NADPH-cytochrome c reductase, or cytochrome P-450 activity, all enzymes associated with microsomal fractions, and their specific activity for NADH-cytochrome c reductase and cytochrome b5, enzymes associated with both microsomal and plasma membranes, is …
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Mechanistic investigation of human cytochromes P450 involved in hormone biosynthesis
CYP17A1 and CYP19A1 are the key cytochromes P450 involved in steroidogenesis. Mutations causing hypo- or hyper-activation of both these enzymes results in diseased states, including cancers. In addition to carrying out mono-oxygenation reactions, both CYP17A1 and CYP19A1 also catalyze carbon-carbon …
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SOTTODOMINI DEL RETICOLO ENDOPLASMATICO IN FISIOLOGIA E PATOLOGIA
… used an endoplasmic reticulum resident protein, cytochrome b5, and an its variant, b5-22, different from the wild-type protein only for its transmembrane domain extended of only 5 aminoacids. Previous studies in my laboratory demonstrated that the b5-22 variant inserts initially in the …
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Emerging anabolic drugs - investigation of the in vitro and in vivo metabolism of selective androgen receptor modulators
… characterised (protein concentration, P450 and cytochrome b5 content, enzymatic activity), and incubated with SARM compounds ostarine, andarine, or S-1 and respective cofactors to generate phase I and II metabolites followed by ultra-high performance liquid chromatography- quadrupole time of …
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Toxicity of mycotoxins to insects and underlying molecular and biochemical mechanisms
… (1-2.5μg/g AFB1) and ochratoxins (1μg/g OTA). Cytochrome P450 monooxygenases (P450s) are critical in detoxification and activation of mycotoxins. In this study, I found that midgut homogenates isolated from H. zea larvae consuming diets supplemented with phytochemicals (coumarin and …
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Mechanismus působení protinádorového léčiva ellipticinu v cílových tkáních jeho účinku
… vlivem ellipticinu ke zvýšení exprese cytochromu b5 a zvýšení exprese a katalytické aktivity CYP3A. Podobně se i obsah a katalytická aktivita NADPH:cytochrom P450 reduktasy měnila v závislosti na premedikaci zvířat ellipticinem. Cytochrom P450 2S1...