Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"CytoF"”.
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Microglial succinate receptor 1 (SUCNR1) sustains chronic CNS inflammation
… seen via ex vivo suspension mass cytometry (CyTOF) immunophenotyping. Mechanistically, I found that SUCNR1 has opposite effects on macrophages, where it attenuates their pro-inflammatory activation, compared to microglia, where it promotes the response to both pro-inflammatory stimuli and …
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Investigating the Role of Hippo/Warts Signaling in Sarcomatoid Renal Cell Carcinoma
… to fulminant tumors in vivo. RNA-sequencing, CyTOF analysis, and implantation studies in immunocompromised mice suggest that Srf drives a pro-tumor microenvironment in Lats1/2 mutant sRCC. Additionally, transcriptional analysis identifies a subset of human RCCs that express an Srf …
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Imaging Post-Infarct Myocardial Inflammation with ⁶⁸Ga-DOTATATE PET/MRI and insights into COVID-19 associated myocardial injury
… subsets using mass cytometry by time-of-flight (CyTOF) and for serum proteomic analysis. <sup>68</sup>Ga-DOTATATE maximum Standardised Uptake Values (SUV<sub>max</sub>) were derived for myocardial segments with late gadolinium enhancement (LGE; “infarct”) as well as for segments without LGE and …
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White Adipose Tissue Remodeling by Intermittent Fasting
… the heterogeneity of WAT depots, we performed CyTOF analysis on stromal and immune cells of inguinal WAT (IWAT) and perigonadal WAT (PWAT) under normal diet (ND) and high-fat diet (HFD). At steady state, IWAT and PWAT exhibited similar immune cell composition, but depot-unique AP …
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Lymphocyte subset variability between human lymphoid tissues
… by mass cytometry (cytometry by time of flight [CyTOF]) for deep phenotypic profiling and tissue-specific subset variation. Populations of B and T cells that had significant abundance differences between the three tissues were identified in an unsupervised manner. The subsets that varied …
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Phenotypic and Functional Characterisation of Hofbauer Cells Across Gestation
… by full term. A high-dimensional mass cytometry (CyTOF) panel was developed and used to show that within the first trimester placenta HBC were a homogeneous population, lacking expression of the HLA class II marker HLA-DR. This contrasted with full-term placenta where HBC displayed heterogeneous …
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Systematic analysis of the molecular mechanisms of T cell entry into tissues
… flow cytometry and cytometry by time of flight (CyTOF). These technologies have made it possible to profile T cells at the single-cell resolution, aiding in the identification of their distinctive transcriptional profile when compared to recirculating T cells as well as tissue-specific features …
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Innate Lymphoid Cell Diversity in Pregnancy and Ovarian Cancer
… are conflicting. Once again applying the CyTOF methodology, I find that there are three major subsets of ascites derived NK cells (aNK). These include previously described CD56bright and CD56dim aNK subsets and an additional NKG2Ahigh KIRlo subset which expresses tissue resident markers …
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A Single Cell Approach to TCR Signal Strength
Cytotoxic T lymphocytes (CTLs) play a key role in the cell-mediated immune response against virally-infected and tumourigenic cells, killing their targets through the release of cytolytic granules. T cell receptor (TCR) recognition of foreign peptides presented by Class I MHC molecules stimulates …
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Il-1Α Blockade Reduces Immune Suppression In The Early Tumor Micro-Environment
<p>IL-1α Blockade Reduces Immune Suppression in the Early Tumor Micro-Environment</p> <p>Brenda Melendez, B.S.</p> <p>Advisory Professor: Gregory Lizee, PhD.</p> <p>Immunotherapy against melanoma has shown great promise in the clinic for treating advanced-stage patients. However, a major barrier …
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Engineering B Cells For Use In Immunotherapy and Cancer
<p>B cells can be divided into effector and regulatory immune cells. While effector B cells are key drivers of humoral immunity due to their ability to generate antibodies specific to pathogens, regulatory B cells (Bregs) have recently been shown to control inflammatory responses in multiple …
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Dynamic Assessment of Nk Cell Interactions With Pediatric Tumor Cells to Predict Response to Immunotherapy
<p>Due to Natural Killer (NK) cells’ capacity to target tumor cells without prior sensitization, adoptive NK cell therapy represents a promising immunotherapy approach for pediatric cancer patients. Our laboratory has developed an NK cell expansion protocol that generates large quantities of NK …