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Showing 1 to 20 of 70 for “"Cysteine protease."”.
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Function and Regulation of Xylem Cysteine Protease 1 and Xylem Cysteine Protease 2 in Arabidopsis
… tracheary element-specific expression of the cysteine protease inhibitor, soyacystatin N in Arabidopsis. Our findings revealed that the absence of XCP2 expression due to T-DNA insertional mutagenesis did not affect plant growth and development in the laboratory. Soyacystatin N was an effective …
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Analysis of a trichomonas vaginalis cysteine protease
… USA. There is increasing focus on the role of cysteine proteases in Trichomonas vaginalis because of their role in virulence of other parasitic protozoa. Determining their location and function will provide insight about their role in the pathogenicity of T. vaginalis and their feasibility as a …
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Inhibition of Cysteine Protease by Platinum (II) Diamine Complexes
… is found to inhibit several enzymes targeting cysteine, histidine and methionine residues, which are expected to be responsible for its anticancer activity. A better understanding of how the size and shape and leaving ligands of platinum complexes affect cysteine protease, papain enzyme are …
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Antimalarial and cysteine protease inhibitor pharmacophores as scaffolds for new antimalarial agents
… mechanism-based inhibiotrs of parasitic cysteine proteases. (iii) Multicomponenet reactions offer the advantage of introducing chemical diversity in fewer steps than conventional multi step organic synthesis. New chloroquine-type compounds were designed and synthesized using the Ugi 4 …
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Promoter Deletion Analysis of Xylem Cysteine Protease 2 (XCP2) in Arabidopsis thaliana
The process of xylem tracheary element differentiation involves the coordination of vascular cambium activity, cell fate determination, cell expansion/elongation, secondary wall synthesis, programmed cell death, and cellular autolysis. The end result of tracheary element differentiation is a …
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Modulation of macrophage function and immune response by a helminth-derived cysteine protease
… One of these Fasciola hepatica products is a cysteine protease, cathepsin L1 (FhCL1), and in fact it comprises a large proportion of the total ES products. In mice, FhCL1 suppresses pro-inflammatory immune responses through cleavage of toll-like receptor (TLR)-3, resulting in modulation of …
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The characterization of cysteine protease 4 and superoxide dismutase 6 in Trichomonas vaginalis
… of Trichomoniasis, is largely unknown although cysteine proteases have been implicated. This paper investigated the role of cysteine protease 4 (CP4) in T. vaginalis through characterization and expression of CP4. T. vaginalis strains showed differential protein and mRNA expression, although it …
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New aminoquinoline antimalarial cysteine protease inhibitors based on the isatin natural product scaffold
… moiety previously demonstrated to inhibit cysteine proteases from mUltiple protozoan parasites. Synthesized compounds were tested against the enzyme falcipain-2 (Rec-FP-2) as well as the parasite source of this protease, Plasmodium Jalciparum (P.f. W2). The results of structure activity …
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Synthesis and biological evaluation of antiparasitic Cysteine protease inhibitors based on the Isatin Scaffold
… the development of new drugs. Meanwhile, the cysteine protease family of enzymes has been identified as potential targets for new modes of chemotherapy due to the numerous critical roles they play in the disease-causing agents. In this project, a non-peptidic and low molecular weight isatin …
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Characterization of cathepsin B cysteine protease from Naegleria fowleri excretory-secretory proteins exerting immunomodulatory effects
… of N. fowleri (Nf-ESPs) include phospholipase, proteases, peroxiredoxins and thrombin receptor. However, the precise mechanism induced by Nf-ESPs in PAM is not fully understood yet. In this study, the cytopathic changes and inflammatory responses induced by Nf-ESPs were analyzed in BV2 …
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Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor
Cystatin C is a cysteine protease inhibitor, aimed for secretion, as it is produced with a signal peptide. Its target enzymes are thought to be the lysosomal cysteine cathepsins and legumain. Cystatin C has been considered to excert its enzyme inhibiting functions extracellularly, as a defense …
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Towards Identifying Cis and Trans Regulators of Expression of Xylem Cysteine Protease 1 (XCP1) in Arabidopsis
… elements. In Arabidopsis thaliana, Xylem Cysteine Protease 1 (XCP1) is specifically expressed in tracheary elements where it catalyzes microautolysis. Thus XCP1 can serve as a useful model for identifying factors that regulate tracheary element-specific gene expression. A deletion analysis …
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Tissue and Cell-Type Localization and Partial Characterization of a Xylem Papain-Type Cysteine Protease From Arabidopsis
Cysteine proteases are associated with xylem tracheary element differentiation. XCP1 was recently identified as a xylem-specific cysteine protease in Arabidopsis (Zhao, et al., 2000). For this study a recombinant polyhistidine-tagged XCP1 (XCP1H6) was expressed and purified from an E. coli …
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Targeting an E3 ubiquitin ligase Siah1 and a cysteine protease SENP1 using SPR and DSF-based fragment screening
… part of the ubiquitination pathway. SENP1 is a cysteine protease that catalyses two essential reactions in the SUMO pathway. It processes pre-SUMO proteins to their mature form and removes SUMO from the target proteins. Siah1 interactions with other proteins involve large surface areas, while …
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Active Center Structure and Sequence Studies on Bacterial Luciferase Utilizing the Essential Cysteine, Protease-Labile Region, and Delta Fragment
… the (alpha) subunit of the enzyme, the essential cysteine and the protease-labile region, which are thought to reside there. Chemical modification of this cysteinyl residue causes slight perturbations, dependent upon the structure of the modifying reagent, in the structure of the protease-labile …
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Investigation of the efficacy of identified acetolactate synthase inhibitors, peptidyl cysteine protease inhibitors, thiolactomycins, and thiosemicarbazone compounds against Mycobacterium tuberculosis
… from the acetolactate synthase inhibitors, cysteine protease inhibitors, thiosemicarbazones, and thiolactomycins classes of compounds for in vitro efficacy against M. tuberculosis using the resazurin microtitre plate assay afterwhich active compounds were assessed for cytotoxicity in vitro …
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An Insight Into the Microbial Diversity and Expression of Cysteine Protease Inhibitors (Cystatin) in <i>Rickettsia parkeri</i> Infected <i>Amblyomma maculatum</i>
<p><em>Amblyomma maculatum</em> (Gulf Coast tick) is an emerging tick species of public health significance in United States. It is a competent vector of <em>Rickettsia parkeri</em>, an etiological agent of a human rickettsiosis. In this study, we investigated the spotted fever group of rickettsial …
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Methodologies For Attaching Polypyridyl Ligands Into Amino Acids And Synthesis And Biological Evaluation Of Novel Light Activated Peptidomimetic Cysteine Protease Inhibitors Caged By Ruii(bpy)2
… presents the synthesis of the first caged cysteine protease inhibitor. After an introductory survey of different Ru and Pt complexes used as potential therapeutic cancer agents, cysteine protease inhibitors are described. The syntheses of RuII(bpy)2 complexes caging cathepsin inhibitors are …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
… the major skeletal muscle protein. However, the protease system responsible for myosin degradation in vivo has not yet been clearly described. Two myosin degrading proteases, a serine protease and a cysteine protease that might have some function in myosin degradation, were isolated from porcine …
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Use of Structure-And Ligand-Based Drug Design Tools for the Discovery of Small Molecule Inhibitors of Cysteine Proteases for the Treatment of Malaria and Sars Infection
… to design and develop inhibitors against cysteine protease of P. Falciparum Malaria and Severe Acute Respiratory Syndrome (SARS). A number of potent inhibitors were developed against cysteine protease and hemoglobinase of P. falciparum , referred as Falcipains (FPs), by the structure-based …
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