Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Cyclodehydratase"”.
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On the biosynthesis and discovery of ribosomally synthesized and post-translationally modified peptides
… is installed over two distinct steps. First, the cyclodehydratase cyclizes an unmodified cysteine, serine, or threonine residue to a thiazoline or oxazoline heterocycle, which can be oxidized by a FMN dependent dehydrogenase to afford a thiazole or oxazole heterocycle. The biosynthesis of a number …
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Elucidation and control of substrate recognition during RiPP biosynthesis
… structurally and functionally diverse products. Cyclodehydratases are a type of RiPP modifying enzyme that catalyze phosphorylation of the peptide backbone and subsequent nucleophilic attack by the sidechains of Cys, Ser, or Thr to form azoline heterocycless (or azoles upon oxidation). The …
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Revealing the molecular details of azoline formation in ribosomal natural product biosynthesis
… roles from each of the proteins in the cyclodehydratase complex (chapter 4 and 5). My findings identify an unprecedented role for ATP and provide the first function to the previously uncharacterized YcaO superfamily of proteins. Furthermore, in order to complete my mechanistic …
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Expanding the structural diversity of the RiPP class of natural products
… This strategy involves the combination of a cyclodehydratase with modification enzymes from different lanthipeptide RiPP classes. These hybrids provide insight into enzyme promiscuity and compatibility, and the biosynthetic order of events, and establish a general platform for the engineering …
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Biosynthetic engineering of plantazolicin natural products
… Bacillus anthracis. During PZN biosynthesis, a cyclodehydratase catalyzes cyclization of cysteine, serine, and threonine residues in the PZN precursor peptide (BamA) to azolines. Subsequently, a dehydrogenase then oxidizes most of these azolines to thiazoles and (methyl)oxazoles. The final …
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A genomics-led approach to deciphering heterocyclic natural product biosynthesis
… PhoxP was proposed to encode an ATP-dependent cyclodehydratase for 5-oxazole formation on an enzyme-bound N-formylglycylacyl-intermediate, and its deletion abolished phthoxazolin production. In vitro reconstitution of the early steps of phthoxazolin biosynthesis was attempted to validate the …
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Biochemical and structural studies on proteins involved in the production of natural products and degradation of polysaccharides
… 2, I discuss the crystallization of the putative cyclodehydratase YcaO. My structural study of YcaO allowed for the identification and characterization of new ATP binding motif present in this family of enzymes. Head to tail macrocyclization is a second type of cyclization observed in serveral …