Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 40 for “"Cyclins"”.
-
Functional Genomic Approaches to Study Cell Polarity Regulation by G1 Cyclins in Saccharomyces cerevisiae
… goal was to dissect the cellular roles of the G1 cyclins by systematically identifying their genetic interactions. To do this, I conducted Synthetic Genetic Array (SGA) screens using strains deleted for different combinations of cyclin genes. The results of screens with strains deleted for the G1 …
-
The Role and Regulation of Cyclin G2 in Human Ovarian Cancer Cells
… direct regulation of the cyclin family. Typical cyclins will accumulate periodically to activate cyclin-dependent kinases (CDKs) leading to the unidirectional flow of the cell cycle, whereas the unconventional G-type cyclins (cyclin G1, G2, and I) act to oppose cell cycle progression. Indeed, …
-
Isolation and characterization of the SIAMESE family: a novel class of cell cycle regulators in plants
… SIM was found to associate with D-type cyclins as well as CDKA;1 in vivo. Although no interactions were detected between SIM and mitotic B-type cyclins, CYCB1;1 is ectopically expressed in sim mutant trichomes. These findings suggest a role for the SIM family in a pathway controlling the …
-
Determination Of The Essential Functions Of A Conserved Cyclin, Cyclin Y, In Drosophila
… encodes a member of a highly conserved family of cyclins, the Y type cyclins, which have not been functionally characterized in any organism. Here I report the generation and phenotypic characterization of a null mutant of <i>CG14939</i>, which we rename <i>Cyclin Y</i> (<i>CycY</i>). I show that …
-
Cell Size Control And Asymmetric Cell Fates In Start Of The Saccharomyces cerevisiae Cell Cycle
… the two modules and showed that different G1 cyclins, CLN3 vs. CLN1 and CLN2, control the two modules. We also showed how positive feedback of G1 cyclins CLN1 and CLN2 on their own transcription ensures a fast transition between the two modules and a coherent commitment to cell cycle …
-
Regulation of Anaphase Promoting Complex Coactivators
… assembly) in CDH1-m11 cells; thus mitotic cyclins are a significant target for Cdh1 with respect to bipolar spindle assembly, and reciprocally, cyclin-Cdk activity is the most significant mechanism for Cdh1 inactivation. Cdc20 has been proposed to be a Cdh1 target, but regulation of Cdc20 …
-
A Genetic and Molecular Analysis of the Control of the Start of the Cell Cycle in Saccharomyces cerevisiae
… its physical association with proteins called cyclins. Budding yeast have three CLN genes (CLNs 1-3), which have limited cyclin homology. At least one of the three is required for cell cycle START. Four B cyclins are known in yeast (CLB5 1-4); two have been shown to function in mitosis. This …
-
Identification of novel regulators of mitotic exit in Saccharomyces cerevisiae
… by the activity of CDK complexed with mitotic cyclins. Upon faithful segregation of a full complement of DNA between each daughter cell, exit from mitosis proceeds. In order for cells to exit from mitosis and enter into G 1, mitotic CDKs must be inactivated. In Saccharomyces cerevisiae, mitotic …
-
Regulation of the mitotic exit network components Tem1 and Cde 15 in Saccharomyces cerevisiae
… cyclin dependent kinase activity (CDK). Mitotic cyclins associated with CDK promote the segregation of genetic material ensuring that each daughter receives a complete complement of the genome. In order for exit from mitosis into G1 to occur, mitotic CDK activity must be inactivated. In the …
-
Regulation of the localization of Lte1, a S. cerevisiae mitotic exit activator
… mitosis is initiated by the action of mitotic cyclins complexed with the cyclin dependent kinase (CDK). Once the chromosomes have been successfully segregated, the exit from mitosis ensues. In order for cells to exit from mitosis, mitotic CDKs must be inactivated. The inactivation of mitotic …
-
Evidenze molecolari del ruolo antiproliferativo e pro-apoptotico della clusterina in cellule epiteliali prostatiche umane
… proliferation markers (i.e. H3 mRNA, PCNA and cyclins A, B1 and D) as detected by RT-qPCR and Western blot. Quite surprisingly, we also found that the turnover of CLU protein is very rapid and tightly regulated by ubiquitin–proteasome mediated degradation. Inhibition of protein synthesis by …
-
Role of retinoblastoma protein in the development of liver cancer
… and apyrimdinic sites. The regulation of three cyclins, cyclin A, cyclin B and cyclin E is found to lack governance. Analysis of the G2/M checkpoint proteins such as Gadd45a, MAD2 and CDC25a all show a dysregulation in their activities. In conclusion Rb loss leads to aberrant regulation of p53 …
-
Temporal organization of the budding yeast cell cycle: general principles and detailed simulations
… of the cell cycle such as: the synthesis of cyclins (Cln1,2; Cln3; Clb1,2; Clb5,6) by their transcription factors (SBF, Mcm1, MBF); their association with stoichiometric inhibitors (Sic1, Cdc6); their degradation by SCF and adaptors of the APC (Cdc20, Cdh1). The emphasis was put on mechanisms …
-
Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
… gene expression and accumulation of mitotic cyclins. I show that partial phosphorylation of Cdh1 allows for partial inactivation of APC-Cdh1. Interestingly, incompletely restrained APC-Cdh1 activity causes a variable phenotype in cell cycle progression on the single cell level. This partially …
-
Investigation of regulatory functions of micrornas in skin and hair follicle development and cycling. A role of microRNA-214 in skin and hair follicle homeostasis.
… in keratinocytes, including inhibition of cyclins and cyclin-dependent kinases and several essential components of Wnt, Edar, Shh and Bmp signalling pathways, whereas -catenin acts as a novel conserved miR-214 target. Indeed, the inhibitory effects of miR-214 on HF development were rescued …
-
Insights into the consequences of chromosome gains and losses in S. cerevisiae
… entry delay due to delayed accumulation of G1 cyclins. Like other cellular stresses, aneuploidy interferes with the cell's ability to grow and to enter the cell cycle. I also developed a novel method to systematically examine the immediate consequences of gaining and-for the first time-losing …
-
Posttranscriptional gene silencing of p27 in primary prostate epithelial cells by small inhibitory RNA (siRNA)
… Cdks (cyclin dependent kinases), along with cyclins, are major control switches for the cell cycle, causing the cell to move from G1 to S or G2 to M. Their function is tightly regulated by Cdk-inhibitors such as the p21 and p27 Cip/Kip proteins. Defects in many of the molecules that regulate …
-
Prelamin A Influences a Program of Gene Expression In Regulation of Cell Cycle Control
… RB-E2F, p53, p27Kip1, FoxOs, p300, and the Cyclins, with additional evidence indicating a role for the Pin1 prolyl isomerase in mediating PreA regulation of the cell cycle.</p>
-
Role of ASCL1 and Its Interactors in Neuroblastoma
… in cell-cycle regulation, such as CDKs and cyclins, or showed weaker association with most of its interactors, including E-protein TCF4, compared to a more responsive cell line. This work suggests understanding ASCL1 interactors could help explain whether this key transcriptional regulator …
Page 1 of 2