Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Cyclic stretch"”.
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Role of microRNA 214 in cyclic stretch induced aortic valve pathogenesis
… be shear-sensitive in AV. However, the effect of cyclic stretch on miR-214 expression and its functional effect in AV are poorly understood. Hence, this work aims to evaluate the effect of cyclic stretch on the expression of miR-214 and one of its well-known targets (ATF4) and downstream genes …
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Cyclic stretch-mediated release of vascular endothelial growth factor by vascular smooth muscle cells : a role in improved vascular graft patency
… of VEGF in contractile cells subjected to cyclic stretch and the profound effects which cyclic stretch has been shown to have on the release of other cytokines by SMC, this study investigates the role which cyclic stretch might play in VEGF expression by SMC in a compliant environment. …
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Engineering approaches to study lung mechanobiology
… of this thesis, I describe the development of a cyclic cell stretching device to mimic the mechanical environment of the pulmonary endothelium during physiological and pathological levels of cyclic stretch. I further varied the cell substrate stiffness in order to replicate the mechanics of soft …
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Characterization of the cardiogenesis of embryonic stem cells
… effects of culture dimensions and uniaxial cyclic stretch on the differentiation process. A smaller culture dimension, with a characteristic length scale of hundreds of micrometers, dramatically enhanced differentiation partly due to an accumulation of cell-secreted and cardiogenic BMP2. …
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Effect of Electrospun Mesh Diameter, Mesh Alignment, and Mechanical Stretch on Bone Marrow Stromal Cells for Ligament Tissue Engineering
… and conditioning them with uniaxial cyclic stretch. These steps were addressed in three complimentary studies to establish new methods to engineer a tissue with ligament-like cells depositing organized extracellular matrix (ECM). In the first study scaffold topographies were …
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Complementary Strategies to Promote Mesenchymal Stem Cell Differentiation for Ligament Tissue Engineering
… of MSCs: scaffold architecture, mechanical stretch and over-expression of the transcription factor, scleraxis. First, elastomeric scaffolds were fabricated by electrospinning a segmented poly(esterurethane urea) with variations in fiber diameter and fiber alignment. Primary mesenchymal stem …
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VE-cadherin mechanotransduction signaling and its regulation by extracellular matrix
… is further examined at the tissue-level, using a cyclic stretch device, as presented in Chapter 4. Using a home-built cyclic stretching device, physiological levels of equibiaxial cyclic stretch (CS) are applied to mimic mechanical stimulation during respiration. Stretch-induced vinculin …
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Mechano-sensitivity of nuclear lamin proteins in endothelial cells
… the same time, endothelial cells are also under stretch by periodic arterial pulses. Considering the fact that atherosclerosis is prone to developing at arterial branches with disturbed shear and increased stretch, it is highly possible that laminar flow and proper stretch force are regulating …
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microRNA-21 as a Pro-Fibrotic Mediator in Right Ventricular Failure
… myocytes from each ventricle were subjected to cyclic stretch and/or aldosterone treatment to mimic mechanical and hormonal stimuli occurring during HF. In RV fibroblasts, miR-21 was increased by both cyclic stretch and by aldosterone, with no significant miR-21 increase in any other cell type. …
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DEVELOPMENT OF A TISSUE ENGINEERED SKELETAL MUSCLE REPAIR CONSTRUCT FEATURING BIOMIMETIC PHYSICAL, CHEMICAL, AND MECHANICAL CUES
… or no expression of AChR clusters. However, cyclic stretch of an agrin-presenting 3D fibrin scaffold seeded with MDCs resulted in dramatic enhancement of AChR clustering relative to samples lacking either agrin or stretch, providing evidence that mechanical strain and agrin supplementation …
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Studying the direct effects of forces on embryonic stem cell behaviour
… technique, and exposed them to single or cyclic stretch. We used this method to study the mechanosensitive response of ES cells. We found that stretching caused an immediate increase in the concentration of intracellular calcium, followed by a rapid decrease in some cells. On timescales of …
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Modulation of mesenchymal stem cell invasion into decellularized engineered tissues
… area developed a novel method to create and stretch tubular cell sheets by seeding neonatal dermal fibroblasts (nHDF) onto a rotating silicone tube. Fibroblasts proliferated to create a confluent monolayer around the tube and a collagenous, isotropic tubular tissue over 4 weeks of static …
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The role of death-associated protein kinase in endothelial apoptosis under fluid shear stress
… as flexible silicone membrane normally used for cyclic strain studies. We have shown a link between shear stress and DAPK expression and apoptosis in cells on membrane. Along with biochemical and molecular signals, the hemodynamic forces that the cells experience are also important regulators of …