Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Cyclic nucleotides"”.
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Measurement of cyclic nucleotides in human sperm: experimental and clinical application
… available to assess essential second messengers. Cyclic nucleotides (cAMP and cGMP) play a crucial role as signalling molecules for capacitation, motility, and acrosome reaction in mammalian sperm. Given their importance in motility and fertilising ability, defective nucleotide function could be a …
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Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility
It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using …
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An investigation into the effect of oxLDL and cyclic nucleotides on platelet cytoskeletal function
Atherosclerosis is a major underlying cause in cardiovascular disease and a significant health concern in developed countries. Part of the mechanism by which platelets are linked to this process is via their activation by oxidised Low-Density Lipoproteins (oxLDL), which drives both atherogenesis …
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Immunohistochemistry for studying cyclic nucleotides in the central nervous system, with particular reference to guanosine 3',5'-monophosphate
… with the aim of histochemically localizing cyclic AMP and cyclic GMP in the C.N.S., and determining the cellular sites where biochemical changes in tissue levels occur. Initial experiments revealed that the inmunoglobulin fraction of non -immune sera bound non -specifically to C.N.S. tissue, …
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Conformational studies of some small biological molecules and their interactions with metal ions
… Dy(III) and Ho (III) complexes of some 3' , 5' cyclic nucleotides were determined quantitatively using NMR line broadening and line shifting techniques. Reactions of Mn(II) with the cyclic nucleotides were used to find the preferred binding sites on the ligands also using line broadening …
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MRP4-Dependent Regulation of Fibroblast Migration
<p>Roles of cyclic nucleotides and cyclic nucleotide-dependent signaling molecules in regulating several signaling pathways including cell migration have long been known. However, the new and revolutionary concept is that it is not just the absence or presence of cyclic nucleotides, but a highly …
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ΦΩΣΦΟΡΥΛΙΩΣΗ ΠΡΩΤΕΙΝΩΝ ΣΕ ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙΠΟΝΤΙΚΟΥ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΜΙΑΣ ΝΕΑΣ ΚΙΝΑΣΗΣ ΠΡΩΤΕΙΝΩΝ ΠΟΥ ΡΥΘΜΙΖΕΤΑΙ ΑΠΟ ΚΥΚΛΙΚΑ ΝΟΥΚΛΕΟΤΙΔΙΑ
… BY THE PRESENCE OF ANY OF THE FOUR 3', 5' CYCLIC NUCLEOTIDES. THIS EFFECT WHICH MAY BE EITHER STIMULATORY OR INHIBITORY, DEPENDS ON THE CONCENTRATION OF ATP, ON THE TYPE OF ACTIVATING METAL EMPLOYED AS WELL AS ON THE SUBSTRATE USED. ON THE BASIS OF THE AFOREMENTIONED EFFECTS OF THE 3', 5' …
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The expression and purification of the regulatory domain of PKG1 alpha
Cyclic guanosine dependent protein kinases play key roles in many functions throughout the body. Three different types of these kinases exist in humans: type one alpha, one beta, and two. The different kinases exist in different locations within the body and also have different substrates that they …
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Role of second generation phosphodiesterase inhibitors on mammalian sperm mobility
… by targeting the modulator and mediator of cyclic nucleotides; (3) develop an animal IVF model to assess effects of Ibudilast on fertilization; and (4) optimise high performance liquid chromatography (HPLC) techniques for routine detection of cyclic nucleotides in sperm cells. A two phase …
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Structural Insights into Ion Selectivity and Calcium Blockage in Cyclic Nucleotide Gated Channels
Cyclic nucleotides-gated (CNG) channels play an essential role in the visual and olfactory sensory systems and are ubiquitously expressed in a variety of neuronal and non neuronal cells. Details of their underlying ion selectivity properties are still not fully understood and a matter of debate in …
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The role of reactive nitrogen species and aged garlic extract on platelet function
… targets within platelets, including calcium and cyclic adenosine monophosphate (cAMP). The effect of AGE upon the other cyclic nucleotide, cyclic guanosine monophosphate (cGMP) is currently unknown. The aims therefore of this project are to identify the effect of AGE upon platelet cGMP, as well …
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Putative changes in dopaminergic neurotransmission following nicotine induced behavioural sensitisation
… assessments, a novel LC-MS/MS method to measure (cyclic-) nucleotides was developed and a fast cyclic voltammetry (FCV) technique was established to measure real-time neurotransmitter release. Specific pharmacological tools were used to identify the role of dopaminergic neurotransmission in …
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Toward the structure and function of carbon-phosphorus lyase enzymes
… both bis-(p-nitrophenyl) phosphate and 2’:3’-cyclic nucleotides as substrates. In collaboration with Dr. Zongchao Jia and Katarenya Podzelinska (Queen’s Biochemistry), we have crystallized phnP and solved the crystal structure at a resolution of 1.3 Å. The structure displayed similarity to …
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Phosphodiesterase 1 (PDE1) at the Crossroads of Calcium and Cyclic Nucleotide Signaling in Diabetic Nephropathy
… of DKD. While renoprotection is mediated by cyclic nucleotides (cAMP and cGMP), phosphodiesterases (PDEs) lead to cyclic nucleotide degradation. Our investigation focused on the role of calcium/calmodulin activated PDE1 in DKD. Three isoforms of PDE1 are differentially expressed in vascular …
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Characterisation of cyclic adenosine monophosphate/protein kinase A signalling networks in blood platelets
… actions remains unclear beyond the triggering of cyclic nucleotides signaling pathways. The aim of this study was to improve our understanding of platelet regulation by cAMP signaling networks.We observed differences in cAMP signaling depending on the agonists used. Using phosphorylation of PKA …
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Functional characterization of Phosphodiesterase-4A in behavior and obesity
… expressed throughout the body that degrade the cyclic nucleotides cAMP and/or cGMP, essentially acting as an off switch for intracellular cAMP and/or cGMP signaling. In particular, one member of this family termed PDE4 is highly expressed in regions of the brain that may play a role in these …
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Regulation of cAMP Signalling to Phospholemman by Phosphodiesterases
The ubiquitous second messenger molecule, cyclic adenosine monophosphate (cAMP), regulates a multitude of cellular functions, with Protein Kinase (PK)A being the major effector of its actions. There is increasing evidence to suggest that phosphodiesterases (PDEs), a class of enzymes that …
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Structure-Function Analysis of Insect Olfactory Receptors
… intracellular second messengers and opening of cyclic nucleotide-gated channels (CNG; Buck and Axel, 1991; Colbert et al., 1997; Firestein et al., 1991; Nakamura and Gold, 1987; Troemel et ii al., 1995). Insect ORs lack similarity to GPCRs (Benton et al., 2006; Vosshall et al., 1999), and we …
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