Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Cyclic GMP"”.
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Molecular Genetic Analysis of Cyclic GMP Signaling in Higher Plants
… are mediated by the small signaling molecule cyclic GMP (cGMP). In addition, sucrose is an inhibitor of phytochrome-regulated gene expression, but the manner in which it achieves these effects remains undefined. It was also found that the signaling pathways of sucrose and phytochrome …
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Roles of Cyclic GMP-AMP Synthase in Immune Defense Against Retroviruses and Autoimmunity
… is known to trigger robust innate immunity. Cyclic GMP-AMP synthase (cGAS) is the sensor of cytosolic DNA and activation of cGAS initiates cytokine production. Here we show cGAS plays essential roles in immune defense against retroviruses as well as in autoimmune diseases caused by self-DNA. …
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Immunohistochemistry for studying cyclic nucleotides in the central nervous system, with particular reference to guanosine 3',5'-monophosphate
… with the aim of histochemically localizing cyclic AMP and cyclic GMP in the C.N.S., and determining the cellular sites where biochemical changes in tissue levels occur. Initial experiments revealed that the inmunoglobulin fraction of non -immune sera bound non -specifically to C.N.S. tissue, …
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An examination of the role of IFI16 in detecting viral DNA in human immortalised keratinocytes
… results in the activation of the DNA sensor cyclic GMP-AMP synthase (cGAS). cGAS produces the second messenger cyclic GMP-AMP (cGAMP) which binds to and activates the endoplasmic reticulum adapter STimulator of INterferon Genes (STING). Activated STING initiates transcription of the …
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Innate Immune Sensing and Signaling of Cytosolic DNA
… a cell-free complementation assay, we identified cyclic GMP-AMP (cGAMP), as a novel eukaryotic second messenger generated by DNA stimulated or DNA virus infected cells. cGAMP contains a unique phosphodiester linkage combination (both 2'-5' and 3'-5'), for which we referred to it as 2'3'-cGAMP. …
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Liquid-Liquid Phase Separations in Innate Immune DNA Sensing and NF-κB Signaling Pathways
The binding of DNA to cyclic GMP-AMP synthase (cGAS) leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP), which activates innate immune responses. We have shown that DNA binding to cGAS robustly induced the formation of liquidlike droplets in which cGAS was activated. The …
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UNVEILING NOVEL CARDIOPROTECTIVE EFFECTS OF GYY4137, A SLOW-RELEASING HYDROGEN SULFIDE DONOR, AND THEIR RELATIONSHIP TO NEUROHORMONAL MODULATION AND MICRORNA PROFILES DURING MYOCARDIAL ISCHEMIC INJURY.
… ANP and BNP in association with enhanced cyclic GMP levels. In addition, GYY treatment had effect on the microRNA profiles. In conclusion, GYY preserves cardiac function, attenuates adverse remodeling and may exert post-ischemic cardioprotective (pro-angiogenic, anti-fibrotic) effects in …
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Studies on the regulation of extracellular collagenase production by vibrio alginolyticus
… by glucose and a number of carbon sources. Cyclic AMP, dibutyryl cyclic AMP and cyclic GMP did not relieve catabolite repression. Glucose and 2-deoxyglucose caused a severe transient repression. No intracellular preformed collagenase was detected and collagenase production ceased when …
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The Role of STING-Mediated Neuroinflammation in Traumatic Brain Injury
… post-mortem brains of human TBI patients. The cyclic GMP-AMP Synthase- Stimulator of Interferon Genes (cGAS-STING) pathway is one of the primary methods of producing Type I IFNs; therefore, this work sought to evaluate the role of the cGAS-STING pathway in a murine controlled cortical impact …
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Multidimensional profiling of the Toxoplasma gondii proteome
… second messenger pathways in T. gondii: calcium, cyclic GMP, and cyclic AMP. The apicomplexan-specific kinase SPARK regulates the AGC kinases PKG, PKA C1, and PKA C3, which together control transitions within the asexual cycle of this important family of parasites.
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Probing the regulatory mechanisms of the actomyosin system in motile cells
… activity by WASP (actin system), the role of cGMP in myosin II assembly (myosin system), and the influence of phosphoinositide signaling (upstream receptor pathway). The model organism chosen for this work was the social ameba Dictyostelium discoideum, due to the well-established knowledge of …
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Thick Ascending Limb Nitric Oxide Production And Inhibition Of Nkcc2 Activity Are Impaired In Angiotensin Ii-Induced Hypertension
… produced by NO synthase type 3 (NOS3) increases cyclic GMP (cGMP) and inhibits THAL transport by reducing Na/K/2Cl cotransporter type 2 (NKCC2) activity. Interestingly, in angiotensin II-induced hypertension, THAL NaCl transport is enhanced. However, whether this is due to a direct effect of …
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Manipulation of second messengers by ectopic expression of receptors in transgenic Drosophila
… selective manipulation of the second messengers cyclic GMP (cGMP), cyclic AMP (cAMP) and calcium (Ca2+) in the renal (Malpighian) tubule of the genetic model organism, Drosophila melanogaster. The importance of the second messengers cGMP, cAMP and Ca2+ in control of fluid transport makes the …
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Direct Measurements of the Effects of Cyclic Adenosine Monophosphate, and Related Compounds, on Membrane Conductances, Intracellular Calcium and Ph in Gastropod Neurons (cyclic-Amp)
… effects of intracellular injections of cyclic adenosine monophosphate (AMP) and related compounds were examined in identifiable gastropod neurons. Injecting cyclic AMP (or cyclic GMP) in amounts which should have elevated the internal concentration from 0.1-7.6 mM (mean = 3.3 mM) …
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Methods and Applications of Computational Design in Multiple States
… design approach to a naturally occurring enzyme, cyclic GMP-AMP synthase (cGAS). Under normal circumstances cGAS adopts an enzymatically active conformation only when bound to double-stranded DNA (dsDNA). By applying multistate design I was able to engineer constitutively active variants of cGAS, …
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Harnessing DNA nanoarchitecture to overcome immunoevasion in cancer
… termed G3YSD, capable of activating the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway; and (2) deliver epigenetic modifiers to reprogram immunosuppressive cues in tumor cells. This solution exploits the modularity, programmability, and ease of control over …
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Exploring antiviral strategies: From innate immunity modulation to viral proteins targeting
… when cytosolic DNA is detected cGAS produces cyclic GMP-AMP (2’3’cGAMP) that directly binds STING determining TBK1 phosphorylation (pTBK1) and hence transcription of IFN-I. On the other side, when the RIG-I pathway is activated in response to viral RNA detection, STING interacts with activated …
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Erythrocyte deformability as assessed by nuclepore filters
… recoverable. Incubations with glucose, dibutyryl cyclic AMP and cyclic GMP, and adenosine triphosphate following shear were made in an attempt to reverse the effects of shear stress. These had little or no effect. Incubation with adenosine, however, was capable of returning sheared cells to their …
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