Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"Curvature sensing"”.
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Understanding Membrane Curvature Sensing
… states to perform their functions. To study the curvature sensitivity of proteins, I have developed a single-particle assay using NanoSight technology that tracks the Brownian motion of particles to measure their size. I optimised this system to track fluorescently labelled lipid-binding domains …
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Development of a Biosensor for Investigating Membrane Curvature Sorting
… was developed to probe the influence of curvature on sorting. This biosensor mimics large, two-dimensional membranes in dynamic equilibrium, achieves high spatial resolution between curvature and molecules of interest, and has high sensitivity, enough for single particle detection. The …
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The bacterial actin-like cell division protein FtsA forms antiparallel double filaments upon binding of FtsN
… in cell elongation. MreB filaments sense curvature and serve as a rudder in the cell elongation complex, ensuring oriented insertion of peptidoglycan around the cell circumference. Following from that I propose that curvature sensing is conserved in FtsA double filaments and, together with …
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Overcoming the plasma membrane barrier to improve the efficiency of therapeutic delivery to the cellular cytoplasm
… endocytosis, I then investigated the curvature sensing abilities of clathrin, a critical question limiting our understanding of how nanoparticles and other molecular cargo are internalized. In particular, my findings demonstrate that clathrin binds preferentially to highly curved …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… process involves significant changes in membrane curvature, and lipids and curvature-sensing proteins can assist these processes. One protein in particular, phospholipase D1 (PLD1), and its product, the lipid phosphatidic acid (PA), are involved in multiple exocytic processes. However, PLD1 and …
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Understanding the membrane biophysics of alpha-Synuclein and its role in membrane curvature induction and structural remodeling
… biophysical studies have identified potential curvature sensing and curvature inducing characteristics for aSyn, however the mechanism behind these processes is not well understood. The work in this thesis explores the membrane remodeling capacity of aSyn using a combination of computational …
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3D Coiling at the Protrusion Tip: New Perspectives on How Cancer Cells Sense Their Fibrous Surroundings
… coiling dynamics are mediated by both the fiber curvature and the metastatic capacity of the cancer cells with less aggressive cancer cells showing diminished coiling. Based on these results, we explore the possibility of using coiling in conjunction with other key biophysical metrics such as …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… phosphates (PIPs) at membranes, and membrane curvature sensing to trigger actin polymerisation via the Arp2/3 complex. While membrane binding is key in in vitro purified reconstitutions of actin polymerisation, the lipid specificity and contribution of each membrane domain is under dispute and …
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Homeostatic mechanics framework for modelling interactions of cells with the microenvironment
… framework to investigate the role of substrate curvature in the orientational ordering of myofibroblasts. We propose that the microtubules are the primary cytoskeletal component involved in curvature sensing. Using detailed morphometric analysis, we confirm our hypothesis, and additionally …