Global ETD Search
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Showing 1 to 9 of 9 for “"Cupriavidus necator"”.
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Improved Polyhydroxyalkanoate Production from Selected Volatile Fatty Acids using Cupriavidus necator
… by pure culture bacterial fermentation using Cupriavidus necator. Optimisation strategies involved the following investigations using shake flasks and 5 litre based batch fermentations:<br/><br/>Shake flask investigations determined that the temperature of 30oC and a nutrient medium, resulted …
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Vibrational spectroscopic studies on [NiFe] hydrogenases: insights into structure and function
… of the membrane-bound [NiFe]-hydrogenase from Cupriavidus necator, lacking the [Fe-S] cluster-containing small subunit HoxK, has been subjected to detailed spectroscopic investigations. This project revealed so far unknown maturation intermediates of the stepwise assembled [NiFe] cofactor. …
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Biosynthesis and characterisation of polyhydroxyalkanoate biopolymers and their oligomers for circular economy
… These plastic wastes were supplied directly to Cupriavidus necator for use as potential carbon sources for PHA accumulation in a 48-hour shake flask cultivation study, in either tryptone soy broth or basal salt medium. LDPE and PE-T produced the most PHA yield with high purity (29% CDW and 40% …
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The bacterial synthesis and characterisation of polyhydroxyalkanoates using waste plastics as carbon sources for novel applications
… potential carbon sources. The bacterial strain Cupriavidus necator H16 was used to generate polyhydroxybutyrate (PHB) and other PHAs with differing polyester compositions, depending on the type of waste carbon source used in 48 hour fermentations in nutrient rich media. These products were then …
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Investigating Microbial Quorum Sensing Potential for Enhanced Production of Biodegradable Polymers
… this study two Gram-negative bacterial species (Cupriavidus necator H16 and Pseudomonas putida KT2440) were grown in specific production media and supplemented with three different exogenous quorum molecules sensing (C4-HSL, C6-HSL and 3-oxo-C12-HSL), to study if there was a change in the amount …
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Biohydrogen as a Fuel: Understanding and Engineering Hydrogenase Enzymes for Biotechnological Applications
… NADH-consuming [NiFe]-hydrogenase, native to Cupriavidus necator. It was shown that this hydrogenase was assembled in E. coli and it exhibited activity both in vitro and in vivo. Serendipitously, it was found that the native E. coli maturase system, critical for biosynthesis of the [NiFe] …
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Producción de precursores de bioplásticos a partir de la coseta de remolacha agotada
… polihidroxialcanoatos mediante fermentación con Cupriavidus necator se evaluó en el hidrolizado de la ESBP, rico en azúcares, y en el medio obtenido después de la fermentación láctica, rico en ácidos orgánicos. Estos experimentos se llevaron a cabo como estudios preliminares, para ver la …
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Production of bioplastic precursors (polyhydroxyalkanoates) from macroalgae
… PHAs, by fermentation with a pure culture of Cupriavidus necator, using as substrate sugar hydrolysates obtained from the alga without pretreatment, and previously pretreated with acid dilutions and heat input. Firstly (article 1), the dietary fibre content of the biomass without pretreatment …