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Showing 1 to 6 of 6 for “"Cupredoxin"”.
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Fine���tuning the reduction potential of Cupredoxin proteins by altering secondary coordination sphere interactions
… A striking example of this is the type 1 (T1) Cupredoxin family of proteins. In this family of proteins, Em values vary across 500‐600 mV, but the redox active copper is ligated in a similar distorted tetrahedral fashion, with identical ligands. The spectroscopic features of these proteins are …
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Expanding the chemistry of a cupredoxin by designing nonnative active sites and using abiological metals
… in both electron transfer proteins, most notably cupredoxins, and enzymes that catalyze oxidative reactions, such as copper-dependent monooxygenases. Cupredoxins are single-domain β-barrel proteins that contain a mononuclear type 1 copper binding site and typically have a strong blue color in …
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Characterization and catalytic application of an engineered cupredoxin possessing a potential in excess of 1 volt
… of the electron transfer catalyst Azurin - a cupredoxin cloned from Pseudomonas aeruginosa - has recently been created, in an attempt to demonstrate the power of the secondary coordination sphere constituents in determining the redox potential of a metal center. This new variant, …
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The role of copper proteins in the ammonia oxidation pathway of Nitrosopumilus maritimus
… with an additional T1Cu site in the C-terminus cupredoxin domain, and Nmar1667 an NO-forming nitrite reductase. Purification of Nmar1131 was performed. Originally over-expression was attempted in a cytoplasmic vector, which proved unsuccessful. To echo the predicted native periplasmic nature of …
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Evolution of copper-containing nitrite reductase
… nitrite reductase (NiR) is a homotrimer of two cupredoxin domains and catalyzes the single electron reduction of NO2- to NO during dissimilatory denitrification. To investigate the evolution of NiR, methods of mutagenic library generation and high-throughput variant screening from E. coli …
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Structural characterization of the two copper proteins nitrous oxide reductase from Pseudomonas stutzeri and laccase Lcc5 from Coprinopsis cinerea
… tetranuclear CuZ-Zentrum und einer C-terminalen Cupredoxin-ähnlichen Domäne, welche ein gemischt-valentes CuA-Zentrum trägt, ähnlich dem der Cytochrom c Oxidase. Diese N2O-Reduktasestrukturen repräsentieren jedoch aerob isoliertes Protein, welches nur aktiv ist, nach einer verlängerten Inkubation …