Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Cumulus cells"”.
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Comparison of precipitating cumulus cells : model-generated versus radar-observed.
Thesis. 1978. M.S.--Massachusetts Institute of Technology. Dept. of Meteorology.
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Transcriptional profiles of cumulus-oocyte complexes related to developmental competence in bovine oocytes
During folliculogenesis, oocytes and cumulus cells undergo many morphological and physiological changes. Transcriptome data were produced from single oocytes and corresponding cumulus cells (CC) to infer the differences in the transcript abundance from fully grown versus growing phase oocytes and …
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The Impact Of Myeloperoxidase And Its Related Oxidants On Metaphase Ii Mouse Oocyte Quality
… of the defense antioxidant capacity of the cumulus oocyte complex (COC). Cumulus cells demonstrated protection against H2O2 and •OH insult at low concentrations, but this protection was lost at higher concentrations and all concentrations of HOCl as judged by changes in the organized compact …
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MATERNAL DETERMINANTS OF OOCYTE AND EMBRYO QUALITY
… oocyte quality, I have compared oocytes and cumulus cells of different qualities at a molecular level. I present in this thesis the pathways and molecules that may determine the developmental competence of oocyte as well as candidate molecular markers of oocyte and embryo quality. A cDNA …
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Modulation of calcium signalling in human sperm by nitric oxide
… to assess the ability of human sperm and cumulus cells (surrounding the oocyte) to generate NO, investigate the mechanism of action of NO, the NO-mediated [Ca\(^{2+}\)]\(_i\) signalling pathways, the possible interaction of NO with progesterone (a product of cumulus) and its impact in the …
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Role of oocyte-secreted factors in prevention of cumulus cell apoptosis and enhancement of oocyte developmental competence.
… factors, OSFs) regulate a broad range of cumulus cell functions. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this thesis was to examine whether cumulus cells exhibit a low incidence of …
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Η έκφραση του γονιδίου CKAP5 σε κοκκώδη κύτταρα γυναικών που υποβάλλονται σε πρωτόκολλα εξωσωματικής
… πρώιμη εμβρυϊκή ανάπτυξη. Τα κοκκώδη κύτταρα (Cumulus Cells, CCs) διαδραματίζουν κρίσιμο ρόλο για την ανάπτυξη και την ωρίμανση του ωαρίου και η μελέτη της γονιδιακής τους έκφραση θα μπορούσε να προσφέρει πολύτιμες πληροφορίες σχετικά με την έκβαση ενός κύκλου εξωσωματικής γονιμοποίησης. Το …
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Factors influencing in vitro maturation of pig oocytes
… on in vitro maturation of pig oocytes. Cumulus oocyte complexes (COC) were obtained by aspiration of 2-5 mm follicles. The oocytes were cultured for 42 hours at 38.5$\sp\circ$C in mTCM-199 supplemented with LH, FSH, follicular fluid, fetal calf serum and estrous gilt serum, alone or in …
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The Effect of Growth Hormone on Pig Embryo Development in Vitro and an Evaluation of Sperm-Mediated Gene Transfer in the Pig
… affected subsequent embryo development. Pig cumulus-oocyte complexes (COC) (n=987) were aspirated from slaughterhouse derived ovaries and cultured in BSA-free NCSU 23 medium containing porcine follicular fluid (10% v/v), cysteine (0.1 mg/ml) and hormonal supplements (eCG and hCG, 10 IU/ml …
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Maintaining Proper Levels of DNA Methylation Marks Through the TET Family is Critical for Normal Embryo Development in Pigs
… presence of TET3 isoforms in porcine oocytes and cumulus cells was investigated to dissect the gene structure of TET3 that could assist in understanding mechanistic actions of TET3 in the DNA demethylation process. Among the three TET3 isoforms identified in cumulus cells, only the pTET3L isoform, …
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Strategies to improve in vitro maturation of human and mouse oocytes
… of oocytes for research in the fields of stem cells and cloning, obtaining oocytes for donation and preservation of fertility. However, several aspects of the technique are still in the process of optimization. A crucial point in this context is the development of new culture strategies for …
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Supplementing IL6, IL11, and LIF to Improve Cultured Bovine Oocyte Competency
… receptors for IL6, IL11, and LIF as present in cumulus cells at the time COCs are removed from their follicles. Consequently, we wanted to investigate if supplementing 25 ng/ml of IL6, IL11, or LIF would improve IVM bovine oocyte competency. Several experiments were completed …
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Genomic studies of oocyte physiology reveal mechanisms critical to oocyte competence
… in the medium regulates TNFAIP6 expression in cumulus cells, and thus influence oocyte development. The other experiment focused on the genes that are related to redox homeostasis. Using same oocyte competence models (prepubertal versus adult, in vivo versus in vitro) we found that GLRX2, …
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The combinative effect of soluble suLeA glycan and specialized bovine semen extender, FSRD4+, on bull sperm quality
… the sperm to hyperactivate and penetrate the cumulus cells and the zona pellucida, and fertilize the oocyte. The sperm-oviduct binding and subsequent sperm reservoir formation is facilitated by glycan-lectin binding. In cattle, the Lewis A trisaccharide (LeA) in the oviduct binds sperm. We …
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IN VITRO EMBRYO PRODUCTION IN WOOD BISON (Bison bison athabascae)
… and in vitro maturational characteristics of cumulus-oocyte complexes (COC) collected from live wood bison during the anovulatory and ovulatory seasons were compared. The stages of nuclear maturation: germinal vesicle (GV), germinal vesicle break down (GVBD), metaphase I (MI) and metaphase II …
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The role of endothelin-2 in ovulation
During ovulation, the oocyte and associated cumulus cells are expelled from the ovary and travel to the ampulla of the oviduct where fertilization occurs. Concurrently, granulosa and theca cells of an ovulating follicle luteinize and form lutein cells of the highly vascular corpus luteum, which …
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SOMATIC SIGNALS REGULATING MRNA TRANSLATION IN MAMMALIAN OOCYTE.
… pathway required the physical presence of cumulus cells (CCs). AKT phosphorylation during rescue IVM of denuded human immature oocytes, as predicted by our model, failed to activate, providing a putative explanation for the suboptimal outcomes of rescue IVM in humans. Third, to understand …