Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 385 for “"Culturing"”.
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Culturing Vallisneria americana for Restoration Efforts
Robust Vallisneria americana was cultured for restoration purposes. Preliminary studies, with various iron treatments, were conducted to ascertain the amount of phosphorous release into the water column from sediments. There was a significant difference in the amount of phosphorous released if …
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Culturing Talk About HIV/AIDS in Ireland
This Thesis is concerned with the ways in which discourses about HIV and AIDS are generated in Ireland and how they are cultured within the various practicums in which they are generated. I discuss this in relation to three practicums: the practicum of microbiological science as taught to …
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Culturing and processing of Dunaliella for enrichment of enzymatic antioxidants
… at 20°C under medium light irradiation. Optimal culturing conditions were also identified for ascorbate, glutathione, phenolic, chlorophyll, carotenoid and protein content. Response Surface Methodology (RSM) was applied to determine the optimal values of light, temperature, nitrogen level, and …
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Amino : a domestic system for synthetic biology and continuous culturing
With the ability to transfer a trait from one creature to another purposefully, synthetic biology is advancing across unforeseen domains. From algae cells that convert carbon dioxide to fuel, biocementation bacteria to terraform mars, and lab-grown meat, synthetic biology offers new materials for …
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Porphyromonas gingivalis: A Methodology for Culturing, Extracting, and Identifying Fimbriated Strains
… there is little information on selectively culturing and identifying P. gingivalis from a sulcal swab. To further the work of establishing a link between the bacterium and these health conditions, a selective and repeatable method had to be established to detect the presence of fimbriated …
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The Effect of Radiation and Repeated Sub-culturing on TGF-β1 Signaling in FRTL-5 Cells
… determine whether radiation and/or repeated subculturing promoted changes in their levels and/or activity.</p> <p>We quantified changes in cellular growth rate using the MTS cell proliferation assay. TGF-β1 ligand and receptor levels were quantified via ELISA, immunocytochemistry and western …
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Assessment of Microbial Biodegradation of Mixed Soil Contaminants at the Santa Susan Field Laboratory Using TRFLP, qPCR, and Culturing
… chain reaction (qPCR), and traditional culturing techniques combined with sequencing of the 16S or ITS regions of the cultured bacteria and fungi. Soil was collected from the Santa Susana Field Laboratory (SSFL), which was the site of numerous liquid-propulsion rocket engine tests as …
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Effect of different culture media and incubation methods on culturing murine embryos in vitro using a semen straw as an alternative receptacle
… lt was concluded that the fertilization and culturing of murine embryos are possible in French semen straws incubated in a bi-gas incubator and in the gaat vagina as an alternative method of fertilizing oocytes and culturing murine embryos in addition TCM-199 and Ham's F10 can bath be used to …
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Development of a Model System for Culturing Neonatal Rat Ventricular Myocytes in vitro to Monitor Integrin Activation in Response to Altered Contractile Force
Hypertrophic cardiomyopathy (HCM) affects 1 in 500 individuals and remains the leading cause of sudden cardiac death (SCD) in young adults. Recent evidence links mutations within sarcomeric proteins to the HCM disease state. The interplay between the forces generated by the contractile apparatus …
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In vitro comparison of equine tendon- and bone marrow-derived cells expanded with FGF-2 prior to culturing with tendon matrix and IGF-I
This study was performed to determine the effects of fibroblast growth factor-2 (FGF-2) on monolayer expansion of equine tendon- and bone marrow-derived cells prior to culture with autogenous acellular tendon matrix and insulin-like growth factor-I (IGF-I). Progenitor cells were isolated from six …
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Roles of cholesterol in the proliferation and differentiation of bovine myoblasts
… calf serum (LPDS) or normal fetal calf serum. Culturing with LPDS did not alter the cytosolic or membrane cholesterol content, or myoblast differentiation, but inhibited myoblast proliferation, compared to culturing with normal fetal calf serum. In the second experiment, myoblasts were cultured …
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Exploring the effects of single and dual phase culturing on the concentrations of Southern Ocean sea-ice algae and transporting living sea-ice algae from the Southern Ocean to land-based research facilities
… transport, after transport and after liquid culturing. Taxonomic distributions of algae varied greatly between initial samples and concentrations ranged from 46 000 to 1 200 000 cells per ml. The hybrid transportation method caused the lowest degree of change in the community compositions and …
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Ecology of Culturable Organisms at Rozel Point, Great Salt Lake, Utah
… microbial physiology. We report here the culturing and characterization of isolates from Rozel Point, located on the northeastern shore of Great Salt Lake. This site was chosen because of the presence of petroleum seeps at Rozel Point and the extreme salinity of the North Arm of GSL. We …
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Experimental investigations of isotopologue fractionation during microbial methanogenesis
… in this thesis explores and develops different culturing methods to test the following hypothesis: hydrogen concentration and redox potential are important controlling factors of carbon (¹³C/¹²C) and hydrogen (D/H) isotope ratios as well as the abundance of methane clumped isotopologues …
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An appraisal of the equivalence of two media proposed for the isolation of cellulolytic rumen bacteria
… is superior to the rich organic medium for the culturing of cellulolytic rumen bacteria and for the culturing of the predominating fora of the rumen.
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Assessment of flagellate diversity at deep-sea hydrothermal vents using the combined approach of culture-dependent and culture-independent methods
… kinetoplastid flagellates and analyzed without culturing by denaturing gradient gel electrophoresis (DGGE) using PCR primers specific to the kinetoplastid clade. By comparing these two different methods of analysis, my goal was to decrease the biases and/or errors inherent in either method alone …
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Optimization of cell adhesion environments for a liver cell bioreactor
… scaffold. Spheroids were formed either by culturing in spinning suspension or on a static, non-adherent surface. In spheroid culture, the addition of extracellular matrix (ECM) signaling through the use of soluble Matrigel or adhesion protein-coated microspheres did not improve hepatocyte …
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Microfluidic device for cytotoxicity screening
… cell culture plates and a large volume of culturing media, overall a time-consuming and resource-intensive process. We have developed a microfluidic device with internal structures that can evenly distribute cells across five chambers and requires only microliter volume of cell culturing …
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