Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 176 for “"Culture model"”.
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A 3-dimensional vascularized cardiac tri-culture model using chitosan nanofiber scaffolds
The development of an in vitro tissue model that can mimic the 3-dimenisonal (3-D) cellular architecture and mosaic of myocardial tissue holds great value for cardiac tissue engineering, modeling, and cardiovascular drug screening applications. The main objective of this project was to develop a …
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Probiotic Modulation of Mucosal Immune Responses in an In Vitro Co-Culture Model
… on cytokine production and TLR expression in monocultures of monocytes, macrophage subsets, and intestinal epithelial cells. Normally, mucosal gut macrophages resemble the M2 subset and fail to express CD14, a co-receptor for LPS signalling. Therefore, probiotic modulation of LPS-induced NF-kB …
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Placental co-culture model reveals Listeria monocytogenes utilization of monocytes for vertical transmission
… across the placental barrier. A novel cell co-culture system comprised of BeWo and HPVEC cell lines was established to model the human placental barrier. Barrier integrity was confirmed using immunostaining of tight junctions and a FITC-dextran permeability assay. The model was then used to …
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The development and validation of a novel patient-derived 3D meningioma cell culture model
… The development of accurate in vitro systems to model the complexity of meningioma pathology is essential for predicting drug response and developing novel therapeutics. Therefore, in this study, I established an easy-to-use in vitro patient-derived meningioma spheroid model that maintained the …
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Microfluidic Dual-Gel Cell Culture Model For Studying Cell Migration Under Controlled Interstitial Flow
… task in traditional single gel-based cell culture models due to the inevitable correlation among various properties of a hydrogel. We overcome this challenge by developing a microfluidic cell culture platform with 3D dual-gel matrices that enable independent control of key matrix …
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An <i>in vitro</i> human 3D co-culture model to study endothelial-astrocyte interactions
… of research is derived from <i>in vitro</i> eo-culture models in which astrocytes are cultured on a stiff, two-dimensional (2D) surface. Three-dimensional (3D) culture models closely mimic the in lJZVO cellular architecture and they bridge the gap between 2D culture models and animal models. …
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Gain-of-Function Effects of Mutant p53 Explored Using a Three-Dimensional Culture Model of Breast Cancer
… we employed a three-dimensional (3D) culture model of breast cancer. When placed in a laminin-rich extracellular matrix, non-malignant mammary epithelial cells form structures highly reminiscent for many aspects of acinar structures found in vivo. On the other hand, breast cancer …
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Establishment of a Long Term Cell Culture Model for Testing Anti-Infectives against Mycobacterium avium subsp. paratuberculosis
… there is no established laboratory animal model for testing therapeutics. When mice are infected with MAP they develop systemic infection and do not mimic disease observed in ruminants. J774A.1 murine macrophages typically have a very short lifespan of about 4-6 days, however MAP infected …
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Developing a co-culture model of ovarian cancer to identify immunotherapies that can overcome TGF-β-induced immunosuppression
… this challenge, we established a simplified co-culture model of the HGSC TME to test immunotherapies and combination strategies capable of overcoming a high-TGF-β environment. ID8 p53-/- Nf1-/- cells were transduced with a GzmB-cleavable Förster resonance energy transfer (FRET) reporter and …
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Pro-oxidative and Pro-inflammatory Mechanisms of Brain Injury in Experimental Animal and 3D Cell Culture Model Systems
… and (2) Development of a three-dimensional (3D) model system in order to mimic in vivo-like microenvironments to further broaden our knowledge in pro-oxidative and pro-inflammatory mechanisms and their cellular responses within 3D constructs. In the first study, we demonstrated that blast …
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Selenotrisulfide Derivative Of Alpha-lipoic Acid: Evaluation In A Cell Culture Model For Potential Use As A Topical Antioxidant
… were administered to skin cells in cell culture models. Topical selenium application to mice has also been shown to reduce UV damage to skin. Although a variety of chemical forms of selenium are available in nutritional supplements, the efficiency by which they are used for selenoprotein …
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Development of 'In vitro' intestinal models to study the pharmacology of drugs affecting the gastrointestinal tract in normal and diseased conditions. Development of a cell culture model for intestinal pharmacology.
… achieved by the development of `in vitro¿ cell culture models. Thus attempts were also made to develop a cell culture model of neonatal intestine to further investigate the action of pharmacologically active agents. The isolation of individual cell populations from segments taken from the …
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Development of 'In vitro' intestinal models to study the pharmacology of drugs affecting the gastrointestinal tract in normal and diseased conditions. Development of a cell culture model for intestinal pharmacology.
… achieved by the development of `in vitro¿ cell culture models. Thus attempts were also made to develop a cell culture model of neonatal intestine to further investigate the action of pharmacologically active agents. The isolation of individual cell populations from segments taken from the …
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Modelling Human Glomerulosclerosis in vitro
… MC proliferation and POD loss. Animal models of glomerulosclerosis often do not reflect disease pathophysiology and 2D glomerular cell monocultures provide limited insight into a disease in which cellular crosstalk and interaction are fundamental. This thesis describes a 3D tri‐culture …
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The effects of BMP4 on different in vitro models for mouse gastrulation and neural induction
… induction to take place. Gastruloids are a 3D culture model that does not require BMP4 to undergo gastrulation-like events. This thesis describes the effects of BMP4 on gastruloids and on a 2D adherent cell culture model for gastrulation, demonstrating the interactions between BMP4, Nodal, Wnt, …
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Melanocyte proliferation in wounded skin in vitro: a histological and immunohistochemical analysis
… of skin using an in vitro skin organ culture model of wound healing. To determine whether the culture model used in this study was reliable, skin explants were cultured over a period of 12 days, fixed and processed for histological analysis. By measuring the lengths of the developing …
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The Identification of the MCHR-1 in J774A.1 Macrophages and The Effect of Melanin-concentrating Hormone on a 3T3-L1 Adipocyte-J774A.1 Macrophage Co-Culture
… a tissue, therefore I planned to establish a co-culture model system consisting of adipocytes and acrophages in our laboratory. First, the J774A.1 macrophage line was acquired. Eventually, successful macrophage culturing technique was learned. Then, I asked whether theses J774A.1 macrophage …
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