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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 251 for “"Culture medium"”.
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Analysis of cellular responses to microwave irradiation in E. coli and change of oxygen level and culture medium in human cancer cell lines using RNA-seq based transcriptomic profiling
… in cancer cell lines in response to different culture conditions using breast cancer cell lines (MCF7) and prostate cancer cell lines (PC3) as the models. Our results in examining the gene expression change in E. coli showed that the non-thermal effects of MWI led to E. coli cells entering the …
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Biomimetic approach to cardiac tissue engineering
… cardiac fibroblasts and myocytes were co-cultured on a scaffold with a parallel channel array that was perfused with culture medium supplemented with synthetic oxygen carrier (PFC emulsion). Presence of the PFC emulsion resulted in significantly higher cell density and improved contractile …
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The effect of Staphylococcus epidermidis on the pH and dissolution of substitute bone graft materials
… – Measured the pH, calcium and phosphate of the culture medium in the presence and absence of the 5 bone substitute materials before and after autoclaving. Part 2 – Measured the pH of the culture medium in the presence and absence of the 5 bone substitute materials before autoclaving and …
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Lipid catabolism in Treponema
… fatty acids for growth in vitro. Serum, added to culture medium, provides a source of long-chain fatty acids. These fatty acids, however, are esterified to triglycerides, phospholipids, and cholesterol. In this study, the major pathways of complex lipid catabolism in five species of Treponema were …
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The role of bacterial vaginosis-associated sialidase on HIV-1 infection
… strains, 3H6 and 2HI, isolated from the FGT were cultured and tested for production of sialidase. Sialidase was purified from the culture medium of these strains by anion-exchange and gel filtration. Sialidase was successfully purified, as confirmed with a sialidase activity assay as well as …
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A comparative study of certain soil-borne cereal pathogens with reference to carbohydrate utilisation and polysaccharide hydrolysis
… and carboxymethylcellulase when grown in a salts medium with an appropriate carbon source. There was little difference in the amounts of enzymes produced by the organisms except for the greatly enhanced endo-poly-rnethylgalacturonase activity when F. culmorum was grown with citrus pectin as the …
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Peroxidase, Protein, and Growth Responses of Cultured Tomato Pith Explants
Tomato pith explants that were cultured on a simple semi-solid medium were analyzed for changes in peroxidase activity, protein, and growth during a seven day period. Changes in the soluble peroxidase fraction were similar to those observed previously in wounded intact tissue. Protein synthesis, …
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The Effects of Exposure to Extremely Low-Amplitude, Low-Frequency Electric Fields on Skeletal Tissue, In Vitro
… was developed were bone cells or bone organ cultures were exposed to a capacitively-coupled electric field of low frequency (8-25 Hz) and extremely low amplitude (estimated 10<sup>-7</sup> V/cm in the culture medium) for 30 minutes.</p> <p>Using this model system it was found that alkaline …
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Somatic embryogenesis in southern and tropical pine species: Loblolly pine (Pinus taeda), Longleaf pine (P. palustris) and Oocarpa pine (P. oocarpa)
… we have established stable embryogenic cultures from high value families of P. taeda and P. palustris using the tissue culture medium 1218 (Pullman et al 2005) in combination with an auxin:citokinin ratio at 10:5 (molar). However, optimization of the protocols for the maturation and …
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Studies of the Expression of Chloroperoxidase: Isolation and Sequence of a Chloroperoxidase Cdna Clone (complementary-Dna)
… at levels exceeding 400 milligrams per liter of culture medium when fructose is provided as the carbon source. Harnessing the cellular machinery which produces chloroperoxidase could provide a powerful method for the production and secretion of heterologous gene products. This report details the …
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Attachment Ability and Melanoma Inhibitory Activity mRNA Expression Level Changes in Murine B16-F10 Melanoma Cells Post Nanosecond Electric Pulses
… To determine cell attachment ability, the culture medium supernatant and attached cells were collected at 6, 12, 18, and 24 h after a single pulse. The live, dead, and total floating cells in the culture medium supernatant were counted. In addition, the live, dead, and total attached cells …
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Growth of Cultured Carrot Cells as Affected by Glyphosate, Asulam and Various Plant Metabolites
… were grown in flasks containing 100 ml defined medium following inoculation with 0.5 g fresh weight of cells. Cells were grown in the presence or absence of the herbicides glyphosate {N-(phosphonomethyl) glycine}, asulam {methyl (4-aminobenzensulphonyl carbamate)} and various plant metabolites …
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Factors affecting bone cell growth and differentiation under differing culture conditions
… in osteoblast differentiation in 2D versus 3D cultures remain unclear. In this study, rat marrow stromal cells (MSCs) were grown both on plastic and in 3D polymer scaffolds and their differentiation into osteoblasts studied. MSCs cultured in a synthetic 3D matrix differentiate faster into …
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Chemolithotrophic nitrate dependent growth of Rhizobium japonicum on carbon monoxide and its relationship to hydrogenase activity
… per ml to a maximum of 2.0 X 10⁸ cells per ml of culture medium. Growth in the absence of CO was substantially less indicating that CO was required for growth. The rate of disappearance of NO₃⁻-nitrogen from the culture medium during growth was correlated with the growth rate. Hydrogen uptake was …
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The microbiology of mining leach liquor regeneration
… by the inorganic ions present in T. ferrooxidans culture medium. The effect of ferrous iron concentrations and mixtures of ferrous and ferric irons on the rate of iron oxidation by T. ferrooxidans in batch culture was determined. The exponential iron oxidation rate was not affected by the ferric …
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rd1 Photoreceptor Degeneration: Photoreceptor Rescue and Role of Metalloproteases in Retinal Degeneration
… S-Transferase alpha (GST-?? in an organ culture paradigm as well as the role of metalloproteases (MMPs) and their tissue inhibitors in photoreceptor degeneration (TIMPs). The rd1 mouse model displays a retina degeneration which starts around day 10 with rod photoreceptor cell death. By 21 …
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Factors influencing in vitro maturation of pig oocytes
… to investigate the effect of different medium supplements, the involvement of steroid hormones and the role of estradiol-17$\beta$ on in vitro maturation of pig oocytes. Cumulus oocyte complexes (COC) were obtained by aspiration of 2-5 mm follicles. The oocytes were cultured for 42 hours …
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Application of in vitro methods to improve soybean resistance to Fusarium solani, the causal agent of sudden death syndrome
… reactions of five soybean cultivars grown on culture medium amended with fungal culture filtrate were compared to the reactions of the same five cultivars inoculated with the fungus under greenhouse conditions. A significant positive correlation was found between sensitivity of calli to the …
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Concurrent detection and isolation of cellular and molecular biomarkers
… cancer cell line (KB) that were added into cell culture medium with known number. This system was further validated by detecting KB cells and FR spiked into healthy human blood to simulate detection of CTCs and protein biomarkers present in cancer patient blood. The potential of this approach in …
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Mineral nutrition of micropropagated plants
… of the mineral nutrition of plants in in vitro cultures were studied in Delphinium 'Princess Caroline' and Hosta 'Fladspen Blue', two species with contrasting growth habits. Initially methods for accurately measuring mineral concentrations within gelled culture media using atomic absorption …
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