Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Cul3"”.
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Insights into BTB-Cul3 Ubiquitin Ligases from the Structures of SPOP-Substrate Complexes
… adaptors. In the largest CRL subfamily, Cul3 binds a BTB domain, and a protein-interaction domain such as MATH recruits substrates for ubiquitination. Here we present biochemical and structural analyses of the MATH and BTB domain containing protein, SPOP, which regulates diverse signaling …
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Vaccinia virus BTB-Kelch proteins
… domains function as adapters for the cullin 3 (Cul3)-based E3 ubiquitylation system. VACV BBKs are not essential for viral replication, but they all affect the lesion size induced by infection in an intradermal murine model. Viruses lacking any one of these BBK proteins also induce increased …
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Mosaic Analysis of Neurogenesis and Neuronal Morphogenesis in Drosophila Learning and Memory Center Mushroom Bodies
… were further characterized. These two genes are Cul3 and Chinmo. Cul3 encodes a scaffold protein for E3 ubiquitin ligase. Phenotypic analysis demonstrates that Cul3 is required for proper axon arborization and dendritic elaboration of MB neurons. Chinmo is a novel BTB-zinc finger protein. …
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Gitelman & Gordon: Mirror image syndromes reveal the roles of WNKs in blood pressure homeostasis and novel anti-hypertensive targets
… the work aimed to phenotype: (1) heterozygous CUL3$^{WT/\Delta403-459}$ mice to investigate a possible vascular contribution to PHAII pathophysiology, (2) homozygous knock-out mice of MO25, a master regulator known to increase SPAK activity up to 100-fold independent of WNKs, and (3) homozygous …
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Analysis of post-translational modifications of the blue light photoreceptor, phot1, by the CRL3NPH3 ubiquitin ligase complex
… protein, can also interact with Cullin 3 (Cul3) as a substrate adapter in a Cul3-based E3 ubiquitin ligase designated CRL3NPH3. We have shown that the CRLNPH3 complex is necessary for the mono/multi- and polyubiquitination of phot1 under high fluence rate conditions in addition to the …
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Mechanisms of repression of the transcription factor NRF2 by KEAP1- and B-TrCP-dependent ubiquitin ligases and how the dysregulation of NRF2 contributes to lung cancer progression
… the impact of mutations in KEAP1, NFE2L2 and CUL3; in terms of their effect on NRF2 activity using the expression of NRF2-target genes as a read out. The following points were highlighted from this analysis; (1) KEAP1 mutations are more prevalent than NFE2L2 and CUL3 mutations in both lung …
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ANALYSIS OF ELECTROPHILE-INDUCED NRF2 GENE ACTIVATION
… protein Keap1, which is an adaptor protein for Cul3-dependent ubiquitination and degradation of Nrf2. However, the links between site-specificity of Keap1 modification by electrophiles and mechanisms of Nrf2 activation are poorly understood. We studied the actions of the prototypical Nrf2 …
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Characterization of the Genes Essential for Mouse Spermatogenesis
… of KLHL10 was reported to directly interact with CUL3 (an ubiquitin E3 ligase), suggesting KLHL10 is involved in the ubiquitination pathway to regulate protein turnover. Using yeast two-hybrid, we screened an adult mouse testis library to identify testicular proteins that can interact with KLHL10 …
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Defining the mechanism of arsenic-induced degradation of PML
… Depletion of the cullin RING ligase scaffold CUL3, and the NEDD8 E3 ligase DCUN1D1 resulted in striking accumulation of PML, suggesting PML may be a substrate of a CUL3 RING ligase complex. Further experiments using the inhibitor of neddylation, MLN4924 support this hypothesis.<br/>PML is …
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Single cell analysis of Keap1-Nrf2 dynamics
… following exposure to a range of inducers the Cul3-Keap1-Nrf2 complex does not dissociate, but remains intact. Furthermore, we found that inducers lead to the accumulation of the Keap1-Nrf2 complex in the ‘closed’ conformation. Interestingly, blockage of the proteasome also leads to the …
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Understanding Autism Pathology: Insights from Genetic Mouse Model Manipulation of KCTD13 and SHANK3
… with increased levels of RhoA, the KCTD13/CUL3 ubiquitin ligase substrate, and is reversed by RhoA inhibition, confirming increased RhoA as one important molecular mechanism. These changes correlate with a decrease in total dendritic length and spine density in area CA1 of the hippocampus, …
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Dual regulation of transcription factor Nrf2 by Keap1 and the beta-TrCP/GSK-3 in cancer
… adaptor protein that recruits of cullin-3 (Cul3) to Nrf2 via its Neh2 domain. Nrf2 is also negatively regulated by phosphorylation by glycogen synthase kinase-3 (GSK-3) causing β-transducin repeat-containing protein (β-TrCP) to ubiquitinate Nrf2 by Skp1-Cul1-F-box (SCF) ubiquitin ligase …