Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"CtIP"”.
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The interaction of CtIP with DNA damage response proteins
… aspects of DNA damage sensing and signalling. CtIP's nuclear localization before and after DNA damage was determined and its colocalization with the other DNA damaged responsive proteins examined. It was found that CtIP associates in vivo with DNA damage sensor proteins such as the MRN complex …
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Structural and biophysical analysis of Human DNA repair protein CtIP
… It is now well-established that mammalian CtIP (CtBP-Interacting Protein) has an important function in DNA Double-Strand Break repair, by promoting the resection of DNA ends in preparation for homologous recombination (HR) and micro-homology mediated end joining (MMEJ). However, the …
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Control de la procesividad de la resección por las proteinas CTIP, BRCA1 y las topoisomerasas del tipo II
… humanas, y tal como esperábamos, la depleción de CtIP, una proteína clave en el proceso de resección, redujo drásticamente la velocidad de la resección. Además, analizando al interactor de CtIP, BRCA1, encontramos que esta interacción no es esencial para el proceso de resección, aunque si regula …
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Crosstalk between DNA end resection and RNA metabolism
… Recombination and Non-Homologous End-Joining. CtIP is a key protein in this response since it promotes faithful repair of DSBs by licensing homologous recombination. Recently, a crosstalk between DNA Damage Response (DDR) and RNA metabolism at different levels has been discovered. Thus, in this …
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SOMATIC MUTATIONS AT FRAGILE ENHANCERS OF LINEAGE- IDENTITY EPITHELIAL GENES IN BREAST CANCER.
… this approach we identified a unique class of CtIP-bound active enhancers, which share the consensus motif of the GRHL1/2 transcription factors and are involved in the establishment and maintenance of the epithelial phenotype. We found that GRHL1/2 bind to these enhancers and recruit CtIP, …
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The circadian Control of DNA end Resection
… anti-resection factor CCAR2, which counteracts CtIP, the key factor permitting DNA end processing. Furthermore, not only do we describe how CRY1 expression levels impact genome stability and modulate cancer response to treatment, but also how time of the day impacts cancer response to radiation …
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Computational Assessment of Axial Compressor Flowfield Instability
… Industries (MHI) with nominal tip clearance (1.2%ctip), that spike-type stall inception is preceded by exponentially growing and circumferentially propagating long wavelength harmonic perturbations upstream of the rotor tip leading edge. These harmonic perturbations directly influence the …
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Understanding The Repair Mechanisms at Ionizing Radiation-induced Damage in The Human Genome
… XRCC1 with the end resection enzymes Mre11 and CtIP. The XRCC1 immunocomplex isolated from U2OS cells had Alt-EJ activity in vitro; this activity was significantly higher in the immunocomplex from pre-irradiated cells. Our studies thus suggest that activation of Alt-EJ proficient repair …
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UNRAVELING THE MOLECULAR MECHANISM OF BRCA2-POLTETA INTERPLAY IN PREVENTING SSDNA GAP ACCUMULATION DURING DNA REPLICATION
… source of DNA damage and we find that MRE11-NBS1-CtIP nuclease is responsible for this process. We further investigate the origin of ssDNA gaps and find that they mainly arise from SMUG1 processing of demethylation intermediates, such as 5hmC, leading to the generation of abasic (AP) sites. …
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Analysis of The RING Domain And BRCT Repeats of BRCA1
… with DNA repair proteins BACH1, ABRAXAS, and CtIP. To test the importance of these two domains we generated cell lines and mouse models with point mutations that either eliminate the E3 ligase activity of Brca1 while maintaining its interaction with Bard1 (I26A) or eliminate its phosphopeptide …
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ATM Deficiency -- A Multifaceted Defect in Lymphocyte Development
… of histone H2AX inhibits the robust CtIP-dependent opening and resection of hairpin-sealed coding ends thereby preventing their aberrant resolution by alternative repair pathways. Finally, in response to Rag DSBs, through the activation of NFkB and other transcription factors, ATM …
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Characterizing the Role of the E3 Ubiquitin Ligase Subunit MAEA in DNA Repair and Replication
… is not the result of an inability to perform CtIP-dependent end resection, which is in fact increased in MAEA null cells. Instead, the HR defect seems to arise during recombination, wherein MAEA null cells are unable to form or maintain RAD51 foci at sites of damage. This portends further …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… changes the confirmation of RCC1. Thus, the MRN-CtIP pathway contributes to Ran-dependent mitotic spindle assembly by modulating RCC1 chromosome association. In summary, my novel findings have revealed a pair of molecular mechanisms not known previously, which are important to the mitosis field.