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Showing 1 to 20 of 39 for “"Cryoprotectants"”.
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Controlled loading of cryoprotectants (CPAS) with cell volume constraints
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Oocyte cryopreservation techniques are considered very important aspects in biomedical research for oocyte and embryo cryopreservation to be used in future in In Vitro Fertilization (IVF) procedure. Oocyte …
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Development of an automated microfluidic system for the loading and unloading of cryoprotectants from mammalian oocytes
Oocyte cryopreservation is an assistive reproductive procedure that has allowed women to overcome infertility and enjoy increased reproductive freedom. Despite a rapid increase in oocyte cryopreservation cycles over the past seven years, success rates both between and within clinics remain highly …
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Impact of cryoprotectants during freeze drying on Lactobacillus plantarum viability and their role in enhancing probiotic storage stability
… of cells over storage. In this study, various cryoprotectants were assessed for their ability to maintain cell integrity and improve yield during the freeze drying dehydration of Lactobacillus plantarum towards a potential topical pharmabiotic formulation. Inulin, sucrose, maltodextrin, and …
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Use Of Cryoprotectants, Prebiotics And Microencapsulation of Bacterial Cells in Improving the Viability of Probiotic Organisms in Freeze-Dried Yoghurt
… and B. longum 536. The effectiveness of adding cryoprotectants and prebiotics in improving the viability of the selected probiotic organisms was investigated. There was a 7% improvement in the viability of L. casei 1520 when cryoprotectant 'UnipectineTM RS 150' was added at 2.5% (w/v). The …
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Oh cryoprotectant, wherefore art thou cryoprotectant? Investigation of the permeation of common cryoprotectants into live rice callus cells by coherent Raman microscopy
… tissue samples are exposed to mixtures of cryoprotectants. Only a few cryoprotectant formulations have been used almost exclusively since their development in the early 1990s, including Plant Vitrification Solution 2 (PVS2) and Plant Vitrification Solution 3 (PVS3). Unfortunately, these …
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Use of cryoprotectants, microencapsulation of bacterial cells and acid and low temperature adaptations in improving viability of probiotic bacteria in fermented frozen dairy desserts
The main aim of this study was to improve the viability of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium spp.) in fermented frozen dairy desserts. In order to improve the viability of probiotic bacteria, initial studies were undertaken to determine the existing levels of …
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A Fluorescence Correlation Spectroscopy Study of the Cryoprotective Mechanism of Glucose on Hemocyanin
… to prevent the damaging effects of cooling, cryoprotectants are used to inhibit ice formation. Common cryoprotectants used today include ethylene glycol, propylene glycol, dimethyl sulfoxide, glycerol, and sugars. However, the mechanism responsible for the effectiveness of these …
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Cryopreservation of eukaryote algae
… 13 algal classes were studied using various cryoprotectants at several concentrations. A cryopreservation protocol involving culturing the algae under low light (17-22 μE m⁻² s⁻¹) on semisolid (I % agar) media, controlled cooling at 1°C min⁻¹, rapid thawing, and the return of cultures to agar …
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The Effect of Arginine Vasotocin on Cryoprotectant Production during Freezing in the Pacific Chorus Frog, Pseudacris Regilla
… way of surviving during wintering months. Use of cryoprotectants is seen in all freeze tolerant species (glucose/glycerol). Synthesis and transportation mechanisms that regulate this process are not yet fully understood. Arginine vasotocin is a neurohypophyseal peptide known to have glycogenolytic …
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The Rational Design and Use of Novel Small-Molecule Ice Recrystallization Inhibitors for the Cryopreservation of Hematopoietic Stem Cells and Red Blood Cells
… storage of cells. The most commonly used cryoprotectants are 10% dimethyl sulfoxide (DMSO) for hematopoietic stem cells (HSCs) and 40% glycerol for red blood cells (RBCs). DMSO fails to protect the functionality of HSCs after cryopreservation and therefore, up to 20% of HSC …
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Concentration of Cryoprotectant in water-in-oil microdroplets for single cell vitrificaton
… is demonstrated to dynamically concentrate cryoprotectants within single cell-containing droplets. These droplets of sizes of about 30 micron diameter were concentrated to 3-4M from a starting concentration of IM in about 300s. The cells are tolerant to this concentration process and do not …
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Influence of mitochondrial membrane potential on the cryopreservation survival of hepatocytes
… and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by freeze-tolerant animals have also been used. A non-metabolizable glucose analog, …
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Sablefish (Anoplopoma fimbria) sperm: The physiology of activation and the development of a cryopreservation protocol
… of a specific ion. The effects of several cryoprotectants including dimethyl sulfoxide, propylene glycol and glycerol at two concentrations (5% and 10%) and three freezing rates (-2.5, -5 and -7.5 °C/min) were tested on the cryopreservation of sperm using a Crysalys cryogenic programmable …
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Using Fluorescent Probes to Study the Location and Interaction of Disaccharides within Lipid Bilayers
… with varying hydrophilicity as potential cryoprotectants for hydrated cells. In order to study the effectiveness of certain disaccharides as cryoprotectants, liposomes were used as a model membrane system. The location and interactions of both sucralose and sucrose within these membranes …
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A surimi-like material made from beef and pork and a novel protease in bovine and porcine skeletal muscle
… by freeze-thawing, and the addition of known cryoprotectants did not show any effect on the gel forming ability.
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New methods for cryopreserving rooster spermatozoa
… its negative effects on sperm. Alternative cryoprotectants with lower molecular weight than glycerol cross the membrane more quickly and induce less osmotic damage. Cryoprotectants that do not need to be removed from spermatozoa prior to insemination may eliminate the need for processing the …
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Optimization of Cryopreservation Protocols for Cardiovascular Tissue
… antibiotic solution for 24 or 48 hrs at 4°C. The cryoprotectants, dimethyl sulfoxide and glycerol were shown to reduce cellular metabolic viability when heart valve leaflets were incubated in RPMI 1640 medium supplemented with 10% fetal calf serum and either 10% Me<sub>2</sub>SO or 15% glycerol …
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The Effect of Organic Resins on Ice Recrystallization
… Both issues can be mitigated with the use of cryoprotectants, chemical additives that are added to a sample prior to preserving it at -80 °C to -196 °C. Moreover, some cryoprotectants exhibit properties of ice recrystallization inhibitors (IRIs), which reduce the growth rate of ice crystals …
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Dielectric Measurements for Organ Cryopreservation
… for measuring the electrical properties of cryoprotectants and of perfused rabbit kidney tissue. Dielectric measurements were made with both time and frequency domain instrumentation, over a frequency range of 50MHz to 2.6GHz, and a temperature range of -30'C to +20'c. The more successful of …
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Application of insect freeze tolerant strategies to the freezing of bovine embryos
… tolerance of this insect due to its content of cryoprotectants and ice nucleating proteins. Spontaneous ice nucleation of the dialyzed hemolymph occurs between -5 C and -11 C up to dilutions of 1:1000. The objectives of this study were to evaluate the effects of seeding temperature (-5 C vs -7 …
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