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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 128 for “"Cryopreservation"”.
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Cryopreservation of pancreatic islets
… limited by the availability of islets. Long-term cryopreservation of islets could partially address this limitation but current cryopreservation protocols, which use the toxic cryoprotectant dimethyl sulphoxide (DMSO), achieve suboptimal cryosurvival and are not clinically used. The aim of this …
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Cryopreservation of eukaryote algae
… cryoprotectants at several concentrations. A cryopreservation protocol involving culturing the algae under low light (17-22 μE m⁻² s⁻¹) on semisolid (I % agar) media, controlled cooling at 1°C min⁻¹, rapid thawing, and the return of cultures to agar media was developed. This protocol produced …
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Dielectric Measurements for Organ Cryopreservation
… such as the kidney. A major difficulty in organ cryopreservation is that of obtaining rapid warming of the frozen tissue, whilst maintaining a uniform temperature profile . Before cooling, the organ is perfused with a 'cryoprotectant', a solution which reduces freezing injury. Warming rates that …
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Cryopreservation of microencapsulated bovine spermatozoa
The ultimate design of a microencapsulated AI dose is to continuously release sperm over a period of time in the female reproductive tract, thus alleviating the need for estrus detection. The objective of Trial 1 was to determine in vitro sperm release times for three microcapsule membranes. Semen …
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Cryopreservation of Domestic Cat Epididymal Spermatozoa
The primary lines of defense in preventing any exotic species from becoming endangered or extinct should be the protection of their habitat and from poaching. For wildlife conservationists, these primary measures are often not feasible, and thus cannot be the only means to prevent extinction. The …
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Cryopreservation of tissue engineered skeletal muscle
… method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator …
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Optimization of Cryopreservation Protocols for Cardiovascular Tissue
… of this research was to determine the effects of cryopreservation on the physical, biochemical and cellular aspects of heart valve tissue. The present study demonstrated that radiolabeled proline accumulation provides a quantitative estimate of total cellular metabolic viability. Radiolabeled …
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High-throughput sperm cryopreservation of aquatic species
… for application of high-throughput sperm cryopreservation to aquatic species. The blue catfish Ictalurus furcatus was selected for research due to its role in production of hybrid catfish with eggs from channel catfish Ictalurus punctatus. A high-throughput cryopreservation pathway was …
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The cryopreservation of <i>Picea sitchensis </i>germplasm
… be implemented using in vitro, culture therefore cryopreservation technology development is imperative such that elite germplasm can be conserved, without compromise to genetic integrity, whilst phenotypic validation of selected genotypes is undertaken.Three explant types were transferred from the …
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Development of Optimal Electromagnetic Resonance Rewarming Technology for Cryopreservation
The development of successful cryopreservation technology has been restricted to small biological materials due to the limited effective rewarming techniques. Either for conventional cryopreservation techniques which remain a certain fraction of ice crystals or vitrification which aims to exclude …
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Sperm cryopreservation and artificial insemination at Groote Schuur Hospital.
The diagnosis and therapy managed by the specialities of infertile couples is traditionally of Gynaecology and Andrology. The latter is a subspeciality, which should combine the knowledge of urologists and gynaecologists in the treatment of sub or infertile men.
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Cell separation and cryopreservation of cord blood fractions for immunotherapeutic applications
… storage temperatures and the effect of delaying cryopreservation was assessed on three sources of HSCs. Cells were found to be better maintained at refrigerated temperatures and to avoid significant losses in potency, delays in cryopreservation should be minimised to <24 hours for bone marrow and …
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Universal robot for automated microinjection with applications in transgenesis and cryopreservation
Microinjection is the process of injecting a small amount of solution into biological organisms at a microscopic level using a glass micropipette. It is a widely utilized technique with a wide range of applications in both fundamental research and clinical settings. However, microinjection is an …
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Lipid Quantification and Cryopreservation of In Vitro Produced Jersey Cattle Embryos
<p>Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have …
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Influence of mitochondrial membrane potential on the cryopreservation survival of hepatocytes
Hepatocytes are widely used in the pharmaceutical and medical fields for drug metabolism studies, bioartificial liver devices, and repopulation of damaged livers as an alternative to transplantation. However, these cells are scarce and difficult to maintain in culture for prolonged periods of time. …
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Effect of seminal plasma on cryopreservation and function of bovine spermatozoa
This study was conducted to :1) determine the effect of seminal plasma (SP) on sperm viability parameters (estimated percent motile sperm, computer-aided percent motile sperm and percent intact acrosomes) and motion characteristics (average path velocity, VAP; curvilinear velocity, VCL; and …
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The utilization of the prostacyclin analogue iloprost in the cryopreservation of stallion spermatozoa
The progress in the stallion semen cryopreservation industry has been slow, creating the need for further research and development directed toward improving post thaw parameters. Most producers utilize glycerol as the primary cryoprotectant when freezing stallion semen, fully knowing that the cell …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
<p>Bovine <em>in vitro</em> produced embryos suffer from poor developmental competence and altered metabolism which hinders their cryotolerance. Overall, the goals of this thesis were to improve oocyte and embryo culture with the antioxidant polydatin and to optimize slow freezing procedures. This …
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