Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Cryogenic Electron Microscopy (Cryo-EM)"”.
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Use solid-state NMR to study the molecular structures of disease-associated peptide aggregates
… information related to their molecular arrangements, aggregating mechanisms, and possible pathological pathways. First, we characterized the thermal aggregate of Fibroblast Growth Factor-1 (FGF-1) with Dr. Blaber’s lab. We found the well-structured region in aggregate comprised the folding …
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Deep learning methods to study structurally heterogeneous macromolecules in vitro and in situ
… average structure. However, recent advances in cryogenic electron microscopy (cryo-EM) provide avenues to determine sets of heterogeneous structures from a single dataset, and simultaneous advances in cryogenic electron tomography (cryo-ET) enable the resolution of macromolecules in their native …
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INVESTIGATION OF CRYOGENIC SURFACE CHARGING DURING OFF-AXIS ELECTRON HOLOGRAPHY
Cryogenic electron microscopy (cryo-EM) is an increasingly popular technique for determining the structure of solutions, especially of those with protein solutes. The ability to immobilize a fluid and attain atomic resolution of solutes within has propelled the technique to the forefront of …
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Structural Characterization of Plaque Seeded Amyloid-β Fibrils by Magic Angle Spinning NMR
… the structures found in the brain. We have demonstrated that we can reproducibly prepare such seeded samples, which display a single set of NMR peaks indicating a single molecular fold of Aβ. Interestingly, the NMR spectra of the plaque seeded samples do not match the previously identified …
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Structure and regulatory mechanism of human uncoupling protein 1
… protein 1 (UCP1) in the mitochondrial inner membrane is responsible for the thermogenic properties of brown adipose tissue. In response to cold exposure, UCP1 is activated by free fatty acids and catalyses proton leak, dissipating the proton motive force, which mitochondria use to drive ATP …
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Cryo-EM studies of class A GPCRs: the thyroid-stimulating hormone receptor and the β1-adrenoceptor
G protein-coupled receptors (GPCRs) are integral membrane proteins with seven transmembrane domains. They are essential in intercellular communication in human physiology and represent important drug targets in human disease. While methodological advances, particularly in cryogenic electron …
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
… AAV engages with human cells and the immune system we hope to create a roadmap of the AAV capsid that can help direct AAV capsid mutations for a more efficient AAV capsid, which can more easily evade the immune system, while maintaining its essential cellular interactions. We have previously …
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Molecular mechanisms of Sorting nexin-9 in membrane triggered actin polymerisation
… into cells and contributes to aberrant actin assemblies in a rare disease of phospho- inositide lipid metabolism, Lowe syndrome. It’s multifaceted nature is attributed to its structure. The SH3-PX-BAR domains of SNX9 are able to integrate inter- actions with phosphatidylinositol phosphates (PIPs) …
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Riemannian geometry for inverse problems in cryogenic electron microscopy
… thesis develops theory and algorithms for a Riemannian geometric approach to inverse problems in cryogenic electron microscopy (Cryo-EM). It is divided into two parts, motivated by the sub problem of orientation estimation and that of modelling of protein dynamics. The thesis is concluded with …
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How is Ataxia-Telangiectasia Mutated Protein Kinase Activated?
… pull-downs, kinase assays and negative stain electron microscopy, I determined that it does not directly cause changes in ATM to activate it. Instead, it acts indirectly by binding to ATM to localise it to the site of damage. Secondly, I detail attempts at elucidating the architecture of the …
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On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
… the DNA damage response at telomeres, which resemble broken DNA ends. Second, due to their location at the end of the chromosome, telomeres present a unique challenge to the canonical DNA replication machinery. The replisome cannot fully copy the terminal repeats, resulting in gradual telomere …
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Aib(2)oxyntomodulin Peptide Molecular Self-Assembly: Smart Nanostructures Toward Long Acting Formulation for Obesity and Diabetes Type 2
… and mechanical parameters affecting the self-assembly process of Aib2-Oxm free peptides as well as the fundamental physical interactions underpinning self-assembly and controlling stability of their fibrillar structures remained to be resolved. A key element to completing our understanding of the …