Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Cryoelectron Microscopy"”.
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A Structural Perspective on Bacterial Transcription
… RNAP escaping from a promoter using a de novo cryoelectron microscopy approach, revealing seven distinct intermediates. We reveal an unanticipated level of structural rearrangement that RNAP undergoes to clear the promoter, including those required to release the initiation factor, <em>σ</em>, …
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Protein Loop Length Estimation From Medium Resolution Cryoem Images
… modeled and deposited to PDB (Protein Databank). Cryoelectron Microscopy (cryoEM) is an important biophysical technique that produces 3D subnanometer resolution images of molecules not amenable to past approaches like x-ray crystallography or nuclear magnetic resonance. De novo modeling is …
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Metabolic biotinylation of the adenoviral capsid: Avidin-based applications and studies of ligand-targeted gene delivery
… studies on Ad-IX-BAP, structural analysis by cryoelectron microscopy and particle reconstruction is presented. The C-terminal BAP fusion was used as a structural tag to visualize the position of IX within the capsid. Results contradict all previous reports on the location of IX and suggest the …
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Cryo-electron microscopy of HPV16 pseudovirions reveal changes in capsid conformation upon furin cleavage
… from cleavage of L2 by exogenous furin using cryoelectron microscopy (cryo-EM). At 5 Å resolution, we observed that furin-cleaved HPV16 PsVs capsids display widespread changes in the arrangement of capsomeres relative to uncleaved control virions. This structural change is relevant because …
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Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… all helices in the available low-resolution cryoelectron microscopy structure published by others, where helices are named A through D, and generate the first model of gap-junctional channels and hemichannels based on experimental structural measurements. In this model, connexin transmembrane …
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The Role of Phosducin-like Protein and the Cytosolic Chaperonin CCT in G beta gamma dimer Assembly
… structure of PhLP:CCT complex has been solved by cryoelectron microscopy. PhLP was found to bind only one of the chaperonin rings with both N- and C-terminal domains. It spans the central folding cavity of CCT and interacts with two opposite sides of the top apical region, inducing the …
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Elucidation of the Functional Architecture of the Early Pre-Ribosomal Processing Machinery in Yeast
… techniques combined with negative stain electron microscopy and biochemical assays we were able to define the composite RNA binding site of UtpA and characterize its molecular architecture in the absence of high-resolution structural data (Chapter II). Subsequent structure determination of the …
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Structures and Mechanisms of Lysosomal Transporters
Lysosomal membrane transporters are indispensable for maintaining lysosomal homeostasis and proper function. Indeed, mutations in these key proteins can lead to debilitating disorders known as lysosomal storage diseases. Cystinosin and Sialin are two such transporters. Both proteins utilize the low …