Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Cryo-electron microscopy studies"”.
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Cryo-electron microscopy studies of pluripotency factor Nanog
… oligomerisation of Nanog drives self-renewal, cryo-electron microscopy and single particle analysis were utilised to determine a 3D structure of Nanog. By performing size exclusion chromatography and analytical ultracentrifugation, it was discovered that Nanog forms large oligomers of 860 kDa …
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Cryo-electron microscopy studies on ovine mitochondrial complex I
… oxidative phosphorylation pathway. It couples electron transfer between NADH and ubiquinone to proton translocation across the inner mitochondrial membrane, contributing to cellular energy production. Complex I is the largest and most elaborate protein assembly of the respiratory chain with a …
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Multi-Modal Regualtion of Actin Networks
… and the cell cortex. Cellular and tissue level studies have shown that more than 150 actin-binding proteins regulate almost every single aspect of actin physiology, such as actin polymerization, actin severing, and actin crosslinking, but at the molecular level, the structural mechanisms by …
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A Structural Perspective of Antibody Neutralization of Dengue Virus
… between epitope and function. Virologic studies had identified that the most potently neutralizing antibodies are against domain III: DIII) of the E protein. We have identified five epitopes within DENV DIII. Our data suggests that the most potently neutralizing antibodies are specific …
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In vitro reconstitution of the human pre-mRNA 3′ end processing machinery and mechanistic insights into endonuclease activation
… of the human complex. Instead, my biochemical studies reveal that RBBP6 is likely recruited to CPSF in an RNA-dependent manner and that it also interacts with the CFIIm cleavage factor complex. My sequence and mutational analyses suggest that the role of RBBP6 in activating the CPSF …
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Structure & Function of Bacterial Transport Machines in their Cellular Context
… levels compared to its parent. To aid structural studies, I optimise the expression of the T1SS using a flow cytometry based sequential design strategy where conditions are iteratively tested via detection of substrate trapped T1SS and updated until no more improvement can be made. I also test …